3D virtual histology of rodent and primate cochleae with multi-scale phase-contrast X-ray tomography Article Swipe
Jannis Justus Schaeper
,
Christoph A. Kampshoff
,
Bettina Wolf
,
Lennart Roos
,
Susann Michanski
,
Torben Ruhwedel
,
Marina Eckermann
,
Alexander Meyer
,
Marcus Jeschke
,
Carolin Wichmann
,
Tobias Moser
,
Tim Salditt
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1038/s41598-025-89431-0
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1038/s41598-025-89431-0
Multi-scale X-ray phase contrast tomography (XPCT) enables three-dimensional (3D), non-destructive imaging of intact small animal cochlea and apical cochlear turns. Here we report on post-mortem imaging of excised non-human primate and rodent cochleae at different $${\upmu }$$ -CT and nano-CT synchrotron instruments. We explore different sample embeddings, stainings and imaging regimes. Under optimized conditions of sample preparation, instrumentation, imaging protocol, and phase retrieval, high image quality and detail level can be achieved in 3D reconstructions. The showcased instrumentation and imaging protocols along with the reconstucted volumes can serve as benchmarks and reference for multi-scale microanatomy and 3D histology. The provided benchmarks and imaging protocols of this work cover a wide range of scales and are intended as augmented imaging tools for auditory research.
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1038/s41598-025-89431-0
- https://www.nature.com/articles/s41598-025-89431-0.pdf
- OA Status
- gold
- Cited By
- 1
- References
- 47
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4408175067
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- OpenAlex ID
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https://openalex.org/W4408175067Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.1038/s41598-025-89431-0Digital Object Identifier
- Title
-
3D virtual histology of rodent and primate cochleae with multi-scale phase-contrast X-ray tomographyWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2025Year of publication
- Publication date
-
2025-03-07Full publication date if available
- Authors
-
Jannis Justus Schaeper, Christoph A. Kampshoff, Bettina Wolf, Lennart Roos, Susann Michanski, Torben Ruhwedel, Marina Eckermann, Alexander Meyer, Marcus Jeschke, Carolin Wichmann, Tobias Moser, Tim SaldittList of authors in order
- Landing page
-
https://doi.org/10.1038/s41598-025-89431-0Publisher landing page
- PDF URL
-
https://www.nature.com/articles/s41598-025-89431-0.pdfDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
-
https://www.nature.com/articles/s41598-025-89431-0.pdfDirect OA link when available
- Concepts
-
Histology, Primate, Rodent, Contrast (vision), Phase contrast microscopy, Tomography, Scale (ratio), Anatomy, Biology, Medicine, Pathology, Computer science, Radiology, Artificial intelligence, Neuroscience, Cartography, Physics, Ecology, Optics, GeographyTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
1Total citation count in OpenAlex
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2025: 1Per-year citation counts (last 5 years)
- References (count)
-
47Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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