A chaotrope-based approach for rapidin vitroassembly and loading of bacterial microcompartment shells Article Swipe
Kyleigh L. Range
,
T M Chiang
,
Arinita Pramanik
,
Joel F. Landa
,
Samuel N. Snyder
,
Xiaobing Zuo
,
David M. Tiede
,
Lisa M. Utschig
,
Eric L. Hegg
,
Markus Sutter
,
Cheryl A. Kerfeld
,
Corie Y. Ralston
·
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1101/2024.10.31.621445
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1101/2024.10.31.621445
Bacterial microcompartments (BMCs) are proteinaceous organelles that self-assemble into selectively permeable shells that encapsulate enzymatic cargo. BMCs enhance catalytic pathways by reducing crosstalk among metabolites, preventing harmful intermediates from leaking into the cytosol, and increasing reaction efficiency via enzyme colocalization. The intrinsic properties of BMCs make them attractive for biotechnological engineering. However, in vivo expression methods for shell synthesis have significant drawbacks that limit the potential design space for these nanocompartments. Here we describe the development of a new, efficient, and rapid method for in vitro assembly of BMC shells from their protein building blocks. Our method enables large-scale construction of BMC shells by utilizing urea as a chaotropic agent to control self-assembly, and provides an approach for encapsulation of both biotic and abiotic cargo under a broad range of reaction conditions. We demonstrate an enhanced level of control over the assembly of BMC shells in vitro and expand the design parameter space for engineering BMC systems with specialized and enhanced catalytic properties.
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- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.1101/2024.10.31.621445
- https://www.biorxiv.org/content/biorxiv/early/2024/11/01/2024.10.31.621445.full.pdf
- OA Status
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- Cited By
- 1
- References
- 46
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4404007477
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4404007477Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1101/2024.10.31.621445Digital Object Identifier
- Title
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A chaotrope-based approach for rapidin vitroassembly and loading of bacterial microcompartment shellsWork title
- Type
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preprintOpenAlex work type
- Language
-
enPrimary language
- Publication year
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2024Year of publication
- Publication date
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2024-11-01Full publication date if available
- Authors
-
Kyleigh L. Range, T M Chiang, Arinita Pramanik, Joel F. Landa, Samuel N. Snyder, Xiaobing Zuo, David M. Tiede, Lisa M. Utschig, Eric L. Hegg, Markus Sutter, Cheryl A. Kerfeld, Corie Y. RalstonList of authors in order
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https://doi.org/10.1101/2024.10.31.621445Publisher landing page
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https://www.biorxiv.org/content/biorxiv/early/2024/11/01/2024.10.31.621445.full.pdfDirect link to full text PDF
- Open access
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YesWhether a free full text is available
- OA status
-
greenOpen access status per OpenAlex
- OA URL
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https://www.biorxiv.org/content/biorxiv/early/2024/11/01/2024.10.31.621445.full.pdfDirect OA link when available
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In vitro, Computational biology, Biology, Chemistry, Cell biology, Biological system, BiochemistryTop concepts (fields/topics) attached by OpenAlex
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1Total citation count in OpenAlex
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2025: 1Per-year citation counts (last 5 years)
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46Number of works referenced by this work
- Related works (count)
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10Other works algorithmically related by OpenAlex
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