A Comprehensive Comparison of Tissue Processing Methods for High-Quality MALDI Imaging of Lipids in Reconstructed Human Epidermis Article Swipe
Maureen Feucherolles
,
William Le
,
Jérôme Bour
,
Carine Jacques
,
Hélène Duplan
,
Gilles Frache
·
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.1021/jasms.3c00185
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.1021/jasms.3c00185
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) has become an important tool for skin analysis, as it allows the simultaneous detection and localization of diverse molecular species within a sample. The use of in vivo and ex vivo human skin models is costly and presents ethical issues; therefore, reconstructed human epidermis (RHE) models, which mimic the upper part of native human skin, represent a suitable alternative to investigate adverse effects of chemicals applied to the skin. However, there are few publications investigating the feasibility of using MALDI MSI on RHE models. Therefore, the aim of this study was to investigate the effect of sample preparation techniques, i.e., substrate, sample thickness, washing, and matrix recrystallization, on the quality of MALDI MSI for lipids analysis of the SkinEthic RHE model. Images were generated using an atmospheric pressure MALDI source coupled to a high-resolution mass spectrometer with a pixel size of 5 μm. Masses detected in a defined region of interest were analyzed and annotated using the LipostarMSI platform. The results indicated that the combination of (1) coated metallic substrates, such as APTES-coated stainless-steel plates, (2) tissue sections of 6 μm thickness, and (3) aqueous washing before HCCA matrix spraying (without recrystallization), resulted in images with a significant signal intensity as well as numerous m/z values. This refined methodology using AP-MALDI coupled to a high-resolution mass spectrometer should improve the current sample preparation workflow to evaluate changes in skin composition after application of dermatocosmetics.
Related Topics
Concepts
Chemistry
MALDI imaging
Mass spectrometry
Mass spectrometry imaging
Sample preparation
Matrix-assisted laser desorption/ionization
Human skin
Analytical Chemistry (journal)
Desorption
Chromatography
Adsorption
Organic chemistry
Genetics
Biology
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1021/jasms.3c00185
- OA Status
- hybrid
- Cited By
- 4
- References
- 60
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4387661635
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4387661635Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1021/jasms.3c00185Digital Object Identifier
- Title
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A Comprehensive Comparison of Tissue Processing Methods for High-Quality MALDI Imaging of Lipids in Reconstructed Human EpidermisWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2023Year of publication
- Publication date
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2023-10-16Full publication date if available
- Authors
-
Maureen Feucherolles, William Le, Jérôme Bour, Carine Jacques, Hélène Duplan, Gilles FracheList of authors in order
- Landing page
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https://doi.org/10.1021/jasms.3c00185Publisher landing page
- Open access
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YesWhether a free full text is available
- OA status
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hybridOpen access status per OpenAlex
- OA URL
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https://doi.org/10.1021/jasms.3c00185Direct OA link when available
- Concepts
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Chemistry, MALDI imaging, Mass spectrometry, Mass spectrometry imaging, Sample preparation, Matrix-assisted laser desorption/ionization, Human skin, Analytical Chemistry (journal), Desorption, Chromatography, Adsorption, Organic chemistry, Genetics, BiologyTop concepts (fields/topics) attached by OpenAlex
- Cited by
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4Total citation count in OpenAlex
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2025: 3, 2024: 1Per-year citation counts (last 5 years)
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60Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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