A Comprehensive Review of Instrumentation and Applications in Post‐Column and In‐Source Derivatization for LC‐MS Article Swipe
Muhammad Nadeem
,
Irshad Hussain
,
Xiao‐An Fu
,
Amjad Ali
,
Dandan Guo
,
Laila Noureen
,
Qamar Subhani
,
Naushad Ahmad
,
Quan‐Fei Zhu
,
Hairong Cui
,
Yu‐Qi Feng
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1002/mas.21930
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1002/mas.21930
Liquid chromatography‐mass spectrometry (LC‐MS) has become an indispensable tool for elucidating molecular structures and quantifying diverse compounds within complex mixtures. Despite its versatility, it faces various challenges such as ion suppression, low sensitivity, analyte instability, and matrix effects, which are being overcome by different kinds of offline and online derivatization techniques to improve specificity and reduce potential interferences. In this context, considerable advancements have been made in reviewing and critically evaluating a wide range of developed methods and techniques; however, little attention has been given to post‐column derivatization (PCD) in LC‐MS. Therefore, this comprehensive review highlights state‐of‐the‐art advancements in LC‐MS with a specific focus on various types of chemical and physical PCD, and in‐source derivatization. It also examines the latest instrumentation developments, highlights methods and influencing factors, and explores applications in food, proteomics, biology, pharmaceuticals, and environmental analysis from the past four decades. Besides, this review critically examines the role of PCD in LC‐MS along with outlining its advantages and disadvantages. Furthermore, special emphasis is also made on prospects and insights for developing more versatile LC‐PCD‐MS techniques and in‐source methodologies, to address ongoing challenges and aim to open new research avenues for analysts.
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Metadata
- Type
- review
- Language
- en
- Landing Page
- https://doi.org/10.1002/mas.21930
- https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/mas.21930
- OA Status
- bronze
- Cited By
- 3
- References
- 247
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4408417813
All OpenAlex metadata
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https://openalex.org/W4408417813Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1002/mas.21930Digital Object Identifier
- Title
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A Comprehensive Review of Instrumentation and Applications in Post‐Column and In‐Source Derivatization for LC‐MSWork title
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reviewOpenAlex work type
- Language
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enPrimary language
- Publication year
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2025Year of publication
- Publication date
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2025-03-12Full publication date if available
- Authors
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Muhammad Nadeem, Irshad Hussain, Xiao‐An Fu, Amjad Ali, Dandan Guo, Laila Noureen, Qamar Subhani, Naushad Ahmad, Quan‐Fei Zhu, Hairong Cui, Yu‐Qi FengList of authors in order
- Landing page
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https://doi.org/10.1002/mas.21930Publisher landing page
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https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/mas.21930Direct link to full text PDF
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YesWhether a free full text is available
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bronzeOpen access status per OpenAlex
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https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/mas.21930Direct OA link when available
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Derivatization, Context (archaeology), Instrumentation (computer programming), Chemistry, Complex matrix, Analyte, Biochemical engineering, Chromatography, Nanotechnology, Data science, Mass spectrometry, Computer science, Engineering, Paleontology, Operating system, Materials science, BiologyTop concepts (fields/topics) attached by OpenAlex
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3Total citation count in OpenAlex
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2025: 3Per-year citation counts (last 5 years)
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10Other works algorithmically related by OpenAlex
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| abstract_inverted_index.ongoing | 183 |
| abstract_inverted_index.special | 163 |
| abstract_inverted_index.various | 26, 106 |
| abstract_inverted_index.ABSTRACT | 0 |
| abstract_inverted_index.Besides, | 144 |
| abstract_inverted_index.LC‐MS. | 91 |
| abstract_inverted_index.analysis | 138 |
| abstract_inverted_index.biology, | 134 |
| abstract_inverted_index.chemical | 109 |
| abstract_inverted_index.context, | 61 |
| abstract_inverted_index.decades. | 143 |
| abstract_inverted_index.effects, | 38 |
| abstract_inverted_index.emphasis | 164 |
| abstract_inverted_index.examines | 118, 148 |
| abstract_inverted_index.explores | 129 |
| abstract_inverted_index.factors, | 127 |
| abstract_inverted_index.however, | 80 |
| abstract_inverted_index.insights | 171 |
| abstract_inverted_index.overcome | 42 |
| abstract_inverted_index.physical | 111 |
| abstract_inverted_index.research | 190 |
| abstract_inverted_index.specific | 103 |
| abstract_inverted_index.(LC‐MS) | 4 |
| abstract_inverted_index.analysts. | 193 |
| abstract_inverted_index.attention | 82 |
| abstract_inverted_index.compounds | 17 |
| abstract_inverted_index.developed | 76 |
| abstract_inverted_index.different | 44 |
| abstract_inverted_index.mixtures. | 20 |
| abstract_inverted_index.molecular | 12 |
| abstract_inverted_index.outlining | 157 |
| abstract_inverted_index.potential | 57 |
| abstract_inverted_index.prospects | 169 |
| abstract_inverted_index.reviewing | 68 |
| abstract_inverted_index.versatile | 175 |
| abstract_inverted_index.Therefore, | 92 |
| abstract_inverted_index.advantages | 159 |
| abstract_inverted_index.challenges | 27, 184 |
| abstract_inverted_index.critically | 70, 147 |
| abstract_inverted_index.developing | 173 |
| abstract_inverted_index.evaluating | 71 |
| abstract_inverted_index.highlights | 96, 123 |
| abstract_inverted_index.structures | 13 |
| abstract_inverted_index.techniques | 51, 177 |
| abstract_inverted_index.elucidating | 11 |
| abstract_inverted_index.influencing | 126 |
| abstract_inverted_index.in‐source | 114, 179 |
| abstract_inverted_index.proteomics, | 133 |
| abstract_inverted_index.quantifying | 15 |
| abstract_inverted_index.specificity | 54 |
| abstract_inverted_index.techniques; | 79 |
| abstract_inverted_index.Furthermore, | 162 |
| abstract_inverted_index.advancements | 63, 98 |
| abstract_inverted_index.applications | 130 |
| abstract_inverted_index.considerable | 62 |
| abstract_inverted_index.instability, | 35 |
| abstract_inverted_index.sensitivity, | 33 |
| abstract_inverted_index.spectrometry | 3 |
| abstract_inverted_index.suppression, | 31 |
| abstract_inverted_index.versatility, | 23 |
| abstract_inverted_index.LC‐PCD‐MS | 176 |
| abstract_inverted_index.comprehensive | 94 |
| abstract_inverted_index.developments, | 122 |
| abstract_inverted_index.environmental | 137 |
| abstract_inverted_index.indispensable | 8 |
| abstract_inverted_index.post‐column | 87 |
| abstract_inverted_index.derivatization | 50, 88 |
| abstract_inverted_index.disadvantages. | 161 |
| abstract_inverted_index.interferences. | 58 |
| abstract_inverted_index.methodologies, | 180 |
| abstract_inverted_index.derivatization. | 115 |
| abstract_inverted_index.instrumentation | 121 |
| abstract_inverted_index.pharmaceuticals, | 135 |
| abstract_inverted_index.chromatography‐mass | 2 |
| abstract_inverted_index.state‐of‐the‐art | 97 |
| cited_by_percentile_year.max | 97 |
| cited_by_percentile_year.min | 96 |
| corresponding_author_ids | https://openalex.org/A5040331037, https://openalex.org/A5068039761 |
| countries_distinct_count | 5 |
| institutions_distinct_count | 11 |
| corresponding_institution_ids | https://openalex.org/I142740786, https://openalex.org/I32597200, https://openalex.org/I4210119942, https://openalex.org/I4210121573, https://openalex.org/I76130692 |
| citation_normalized_percentile.value | 0.92075853 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | True |