A dual fluorescence channel RAA-based CRISPR-Cas12a/Cas13a system for highly sensitive detection of Gyrovirus galga1 and Gyrovirus homsa1 Article Swipe
Dan Yu
,
Zhixun Xie
,
Yanfang Zhang
,
Zhiqin Xie
,
Qing Fan
,
Sisi Luo
,
Liji Xie
,
Meng Li
,
Tingting Zeng
,
Minxiu Zhang
,
Xiaofeng Li
,
You Wei
,
Aiqiong Wu
,
Li‐Jun Wan
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1080/21505594.2025.2521012
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1080/21505594.2025.2521012
Gyrovirus galga1 (GyG1) and Gyrovirus homsa1 (GyH1) are the second and third most common gyroviruses identified, respectively, after chicken anaemia virus. They were first reported in 2011 and are currently prevalent worldwide. However, limited research on these pathogens and a lack of prevention and control strategies have necessitated the establishment of a rapid diagnostic technique to address new challenges in infectious diseases. Recombinase acid amplification (RAA) combined with CRISPR - Cas12a or CRISPR - Cas13a technology has major advantages for highly sensitive and rapid diagnosis. Specific targets can activate CRISPR-Cas trans-cleavage activity, resulting in non-specific cleavage of single-stranded DNA by the CRISPR - Cas12a complex and RNA cleavage by the CRISPR - Cas13a complex. In this study, for the first time, we combined RAA-based CRISPR - Cas12a and CRISPR - Cas13a systems for simultaneous differential diagnosis of GyG1 and GyH1 infection. The results showed that dual fluorescence channel RAA-based CRISPR - Cas12a/Cas13a technology could detect GyG1 and GyH1 within one hour, with a minimum detection limit of 1.5 copies of the target DNA standard/µL and no cross-reactivity with other avian pathogens. In addition, this method could be used for clinical detection, with the results exhibiting high consistency with those obtained by qPCR. These findings demonstrate that our RAA-based CRISPR - Cas12a/Cas13a dual-channel detection system can detect two different subtypes of gyrovirus in a sample with good specificity and high sensitivity, improving the detection efficiency and providing a new technique for the study of viral infection dynamics.
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1080/21505594.2025.2521012
- OA Status
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- References
- 50
- Related Works
- 10
- OpenAlex ID
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Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4411522884Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1080/21505594.2025.2521012Digital Object Identifier
- Title
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A dual fluorescence channel RAA-based CRISPR-Cas12a/Cas13a system for highly sensitive detection of Gyrovirus galga1 and Gyrovirus homsa1Work title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2025Year of publication
- Publication date
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2025-06-22Full publication date if available
- Authors
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Dan Yu, Zhixun Xie, Yanfang Zhang, Zhiqin Xie, Qing Fan, Sisi Luo, Liji Xie, Meng Li, Tingting Zeng, Minxiu Zhang, Xiaofeng Li, You Wei, Aiqiong Wu, Li‐Jun WanList of authors in order
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https://doi.org/10.1080/21505594.2025.2521012Publisher landing page
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YesWhether a free full text is available
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goldOpen access status per OpenAlex
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https://doi.org/10.1080/21505594.2025.2521012Direct OA link when available
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CRISPR, Biology, Computational biology, Fluorescence, Molecular biology, Genetics, Gene, Physics, Quantum mechanicsTop concepts (fields/topics) attached by OpenAlex
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0Total citation count in OpenAlex
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