A dual-purification system to isolate mitochondrial subpopulations Article Swipe
Corey N. Cunningham
,
Jonathan G. Van Vranken
,
Jakeline Larios
,
Katarina Heyden
,
Steven P. Gygi
,
Jared Rutter
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1242/jcs.263693
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1242/jcs.263693
Mitochondria perform diverse functions, including producing ATP through oxidative phosphorylation, synthesizing macromolecule precursors, maintaining redox balance among many others. Given this diversity of functions, we and others have hypothesized that cells maintain specialized subpopulations of mitochondria. To begin addressing this hypothesis, we developed a new dual-purification system to isolate subpopulations of mitochondria for chemical and biochemical analyses. We used APEX2 proximity labeling such that mitochondria were biotinylated based on proximity to another organelle. All mitochondria were isolated by an elutable MitoTag-based affinity precipitation system. Biotinylated mitochondria were then purified using immobilized avidin. We used this system to compare the proteomes of endosome- and lipid droplet-associated mitochondria in U-2 OS cells, which demonstrated that these subpopulations were indistinguishable from one another but were distinct from the global mitochondria proteome. Our results suggest that this purification system could aid in describing subpopulations that contribute to intracellular mitochondrial heterogeneity, and that this heterogeneity might be more substantial than previously imagined.
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1242/jcs.263693
- OA Status
- hybrid
- Cited By
- 2
- References
- 42
- Related Works
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- OpenAlex ID
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All OpenAlex metadata
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- OpenAlex ID
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https://openalex.org/W4408413903Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1242/jcs.263693Digital Object Identifier
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A dual-purification system to isolate mitochondrial subpopulationsWork title
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articleOpenAlex work type
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enPrimary language
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2025Year of publication
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2025-03-13Full publication date if available
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Corey N. Cunningham, Jonathan G. Van Vranken, Jakeline Larios, Katarina Heyden, Steven P. Gygi, Jared RutterList of authors in order
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https://doi.org/10.1242/jcs.263693Publisher landing page
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YesWhether a free full text is available
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hybridOpen access status per OpenAlex
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https://doi.org/10.1242/jcs.263693Direct OA link when available
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Mitochondrion, Biotinylation, Biology, Oxidative phosphorylation, Proteome, Organelle, Cell biology, BiochemistryTop concepts (fields/topics) attached by OpenAlex
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2Total citation count in OpenAlex
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2025: 2Per-year citation counts (last 5 years)
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10Other works algorithmically related by OpenAlex
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