A dual‐probe one‐pot RPA ‐CRISPR /Cas12a: a highly sensitive and rapid method for detection of Phytophthora vignae
Article Swipe
Mingze Lin
,
Zhiming Zhou
,
Zhiting Li
,
Wanzhen Feng
,
Chengdong Yang
,
Zaobing Zhu
,
Qinghe Chen
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1002/ps.8932
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1002/ps.8932
BACKGROUND Phytophthora vignae is the primary pathogen affecting cowpea plants. Early on‐site and accurate detection of the causal pathogen is critical for successful management. Therefore, it is both essential and urgent to create a rapid, sensitive, and accurate detection for P. vignae in cowpeas. RESULTS In this study, the one‐pot RPA‐CRISPR/Cas12a assay demonstrated the ability to effectively differentiate closely related oomycete species, exhibiting excellent specificity. The entire detection process can be completed within 15–20 min, with results being visually determined using a lateral flow strip (LFS) or by fluorescence detection under blue or ultraviolet (UV) light. Additionally, we have added dual probes to the one‐pot RPA‐CRISPR/Cas12a assay detection system, this modification improved reaction efficiency and significantly enhanced sensitivity (200 fg μL −1 ). CONCLUSION This study developed a dual‐probe one‐pot RPA‐CRISPR/Cas12a detection platform that demonstrates rapid and highly sensitive identification of P . vignae . The proposed technology enables field‐deployable detection without requiring specialized technical expertise, representing a promising approach with substantial potential for practical applications. © 2025 Society of Chemical Industry.
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1002/ps.8932
- https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ps.8932
- OA Status
- bronze
- Cited By
- 1
- References
- 50
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4410752380
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- OpenAlex ID
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https://openalex.org/W4410752380Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1002/ps.8932Digital Object Identifier
- Title
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A dual‐probe one‐pot
RPA ‐CRISPR /Cas12a: a highly sensitive and rapid method for detection ofPhytophthora vignae Work title - Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2025Year of publication
- Publication date
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2025-05-26Full publication date if available
- Authors
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Mingze Lin, Zhiming Zhou, Zhiting Li, Wanzhen Feng, Chengdong Yang, Zaobing Zhu, Qinghe ChenList of authors in order
- Landing page
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https://doi.org/10.1002/ps.8932Publisher landing page
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https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ps.8932Direct link to full text PDF
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YesWhether a free full text is available
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bronzeOpen access status per OpenAlex
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https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ps.8932Direct OA link when available
- Concepts
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CRISPR, Computational biology, Phytophthora, Oomycete, Pathogen, Ultraviolet, Biology, Fluorescence, Chemistry, Nanotechnology, Microbiology, Biochemistry, Botany, Physics, Materials science, Gene, Optoelectronics, Quantum mechanicsTop concepts (fields/topics) attached by OpenAlex
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1Total citation count in OpenAlex
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2025: 1Per-year citation counts (last 5 years)
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10Other works algorithmically related by OpenAlex
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