A high-throughput radioactivity-based assay for screening SARS-CoV-2 nsp10-nsp16 complex Article Swipe
Aliakbar Khalili Yazdi
,
Fengling Li
,
Kanchan Devkota
,
Sumera Perveen
,
Pegah Ghiabi
,
Taraneh Hajian
,
Albina Bolotokova
,
Masoud Vedadi
·
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.1101/2021.02.03.429625
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.1101/2021.02.03.429625
Frequent outbreaks of novel coronaviruses (CoVs), highlighted by the current SARS-CoV-2 pandemic, necessitate the development of therapeutics that could be easily and effectively administered world-wide. The conserved mRNA-capping process enables CoVs to evade their host immune system and is a target for antiviral development. Nonstructural protein (nsp) 16 in complex with nsp10 catalyzes the final step of coronaviral mRNA-capping through its 2’-O-methylation activity. Like other methyltransferases, SARS-CoV-2 nsp10-nsp16 complex is druggable. However, the availability of an optimized assay for high-throughput screening (HTS) is an unmet need. Here, we report the development of a radioactivity-based assay for methyltransferase activity of nsp10-nsp16 complex in a 384-well format, and kinetic characterization, and optimization of the assay for HTS (Z′-factor: 0.83). Considering the high conservation of nsp16 across known CoV species, the potential inhibitors targeting SARS-CoV-2 nsp10-nsp16 complex may also be effective against other emerging pathogenic CoVs.
Related Topics
Concepts
Methyltransferase
Druggability
Biology
High-throughput screening
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Messenger RNA
Computational biology
Coronavirus disease 2019 (COVID-19)
Virology
Methylation
Genetics
Medicine
Gene
Disease
Infectious disease (medical specialty)
Pathology
Metadata
- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.1101/2021.02.03.429625
- https://www.biorxiv.org/content/biorxiv/early/2021/02/03/2021.02.03.429625.full.pdf
- OA Status
- green
- Cited By
- 3
- References
- 42
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3126607922
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W3126607922Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.1101/2021.02.03.429625Digital Object Identifier
- Title
-
A high-throughput radioactivity-based assay for screening SARS-CoV-2 nsp10-nsp16 complexWork title
- Type
-
preprintOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2021Year of publication
- Publication date
-
2021-02-03Full publication date if available
- Authors
-
Aliakbar Khalili Yazdi, Fengling Li, Kanchan Devkota, Sumera Perveen, Pegah Ghiabi, Taraneh Hajian, Albina Bolotokova, Masoud VedadiList of authors in order
- Landing page
-
https://doi.org/10.1101/2021.02.03.429625Publisher landing page
- PDF URL
-
https://www.biorxiv.org/content/biorxiv/early/2021/02/03/2021.02.03.429625.full.pdfDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
greenOpen access status per OpenAlex
- OA URL
-
https://www.biorxiv.org/content/biorxiv/early/2021/02/03/2021.02.03.429625.full.pdfDirect OA link when available
- Concepts
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Methyltransferase, Druggability, Biology, High-throughput screening, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Messenger RNA, Computational biology, Coronavirus disease 2019 (COVID-19), Virology, Methylation, Genetics, Medicine, Gene, Disease, Infectious disease (medical specialty), PathologyTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
3Total citation count in OpenAlex
- Citations by year (recent)
-
2024: 1, 2021: 2Per-year citation counts (last 5 years)
- References (count)
-
42Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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