A High-Throughput Screening Procedure (Py-Fe3+) for Enhancing Ethanol Production by Saccharomyces cerevisiae Using ARTP Random Mutagenesis Article Swipe
Weiyang Wang
,
Shihua Wu
,
Yuan-Han Xie
,
Miao Zhong
,
Man-Li Wei
,
Zeyang Li
,
Xiufeng Long
,
Fu-Xing Niu
·
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.3390/pr10112186
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.3390/pr10112186
Saccharomyces cerevisiae is an important microbial organization involved in ethanol synthesis. Mutant strains that can withstand multiple pressures during this process are critical to the industrial development of biofuels. In this study, a dual high-throughput screening method of Triphenyl-2H-tetrazoliumchloride (TTC)-based macroscopic observation and the reaction of ferric nitrate with pyruvate (or pyruvate radical ion) in fermentation broth was used. Using this, an S. cerevisiae mutant library that could tolerate 381 g/L sucrose was established by ARTP random mutation and adaptive evolution to select the best strain; its ethanol yield was increased by an additional 20.48%; and the sucrose utilization rate was 81.64%. This method is specific to the selection of strains with increased ethanol production.
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3390/pr10112186
- https://www.mdpi.com/2227-9717/10/11/2186/pdf?version=1667806892
- OA Status
- gold
- Cited By
- 10
- References
- 21
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4307549179
All OpenAlex metadata
Raw OpenAlex JSON
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https://openalex.org/W4307549179Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.3390/pr10112186Digital Object Identifier
- Title
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A High-Throughput Screening Procedure (Py-Fe3+) for Enhancing Ethanol Production by Saccharomyces cerevisiae Using ARTP Random MutagenesisWork title
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2022Year of publication
- Publication date
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2022-10-25Full publication date if available
- Authors
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Weiyang Wang, Shihua Wu, Yuan-Han Xie, Miao Zhong, Man-Li Wei, Zeyang Li, Xiufeng Long, Fu-Xing NiuList of authors in order
- Landing page
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https://doi.org/10.3390/pr10112186Publisher landing page
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https://www.mdpi.com/2227-9717/10/11/2186/pdf?version=1667806892Direct link to full text PDF
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goldOpen access status per OpenAlex
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https://www.mdpi.com/2227-9717/10/11/2186/pdf?version=1667806892Direct OA link when available
- Concepts
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Saccharomyces cerevisiae, Mutagenesis, Ethanol, Fermentation, Sucrose, Chemistry, Biochemistry, High-throughput screening, Strain (injury), Ethanol fuel, Mutant, Yield (engineering), Biology, Yeast, Gene, Materials science, Anatomy, MetallurgyTop concepts (fields/topics) attached by OpenAlex
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10Total citation count in OpenAlex
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2025: 1, 2024: 7, 2023: 2Per-year citation counts (last 5 years)
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21Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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