A highly efficient bead extraction technique with low bead number for digital microfluidic immunoassay Article Swipe
Cheng-Yeh Huang
,
Po-Yen Tsai
,
I‐Chin Lee
,
Hsin‐Yun Hsu
,
Hong-Yuan Huang
,
Shih‐Kang Fan
,
Da‐Jeng Yao
,
Cheng‐Hsien Liu
,
Wensyang Hsu
·
YOU?
·
· 2016
· Open Access
·
· DOI: https://doi.org/10.1063/1.4939942
YOU?
·
· 2016
· Open Access
·
· DOI: https://doi.org/10.1063/1.4939942
Here, we describe a technique to manipulate a low number of beads to achieve high washing efficiency with zero bead loss in the washing process of a digital microfluidic (DMF) immunoassay. Previously, two magnetic bead extraction methods were reported in the DMF platform: (1) single-side electrowetting method and (2) double-side electrowetting method. The first approach could provide high washing efficiency, but it required a large number of beads. The second approach could reduce the required number of beads, but it was inefficient where multiple washes were required. More importantly, bead loss during the washing process was unavoidable in both methods. Here, an improved double-side electrowetting method is proposed for bead extraction by utilizing a series of unequal electrodes. It is shown that, with proper electrode size ratio, only one wash step is required to achieve 98% washing rate without any bead loss at bead number less than 100 in a droplet. It allows using only about 25 magnetic beads in DMF immunoassay to increase the number of captured analytes on each bead effectively. In our human soluble tumor necrosis factor receptor I (sTNF-RI) model immunoassay, the experimental results show that, comparing to our previous results without using the proposed bead extraction technique, the immunoassay with low bead number significantly enhances the fluorescence signal to provide a better limit of detection (3.14 pg/ml) with smaller reagent volumes (200 nl) and shorter analysis time (<1 h). This improved bead extraction technique not only can be used in the DMF immunoassay but also has great potential to be used in any other bead-based DMF systems for different applications.
Related Topics
Concepts
Bead
Chromatography
Microfluidics
Analyte
Magnetic bead
One-Step
Digital microfluidics
Immunoassay
Electrowetting
Extraction (chemistry)
Materials science
Detection limit
Electrode
Chemistry
Nanotechnology
Chemical engineering
Composite material
Biology
Physical chemistry
Engineering
Antibody
Immunology
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1063/1.4939942
- https://aip.scitation.org/doi/pdf/10.1063/1.4939942
- OA Status
- bronze
- Cited By
- 23
- References
- 45
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W2239187555
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W2239187555Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.1063/1.4939942Digital Object Identifier
- Title
-
A highly efficient bead extraction technique with low bead number for digital microfluidic immunoassayWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2016Year of publication
- Publication date
-
2016-01-01Full publication date if available
- Authors
-
Cheng-Yeh Huang, Po-Yen Tsai, I‐Chin Lee, Hsin‐Yun Hsu, Hong-Yuan Huang, Shih‐Kang Fan, Da‐Jeng Yao, Cheng‐Hsien Liu, Wensyang HsuList of authors in order
- Landing page
-
https://doi.org/10.1063/1.4939942Publisher landing page
- PDF URL
-
https://aip.scitation.org/doi/pdf/10.1063/1.4939942Direct link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
bronzeOpen access status per OpenAlex
- OA URL
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https://aip.scitation.org/doi/pdf/10.1063/1.4939942Direct OA link when available
- Concepts
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Bead, Chromatography, Microfluidics, Analyte, Magnetic bead, One-Step, Digital microfluidics, Immunoassay, Electrowetting, Extraction (chemistry), Materials science, Detection limit, Electrode, Chemistry, Nanotechnology, Chemical engineering, Composite material, Biology, Physical chemistry, Engineering, Antibody, ImmunologyTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
23Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 1, 2024: 1, 2023: 2, 2022: 2, 2021: 2Per-year citation counts (last 5 years)
- References (count)
-
45Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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