A Mating Procedure for Genetic Transfer of Integrative and Conjugative Elements (ICEs) of Streptococci and Enterococci Article Swipe
Francesco Iannelli
,
Francesco Santoro
,
Valeria Fox
,
Gianni Pozzi
·
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.3390/mps4030059
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.3390/mps4030059
DNA sequencing of whole bacterial genomes has revealed that the entire set of mobile genes (mobilome) represents as much as 25% of the bacterial genome. Despite the huge availability of sequence data, the functional analysis of the mobile genetic elements (MGEs) is rarely reported. Therefore, established laboratory protocols are needed to investigate the biology of this important part of the bacterial genome. Conjugation is a mechanism of horizontal gene transfer which allows the exchange of MGEs among strains of the same or different bacterial species. In streptococci and enterococci, integrative and conjugative elements (ICEs) represent a large part of the mobilome. Here, we describe an efficient and easy-to-perform plate mating protocol for in vitro conjugative transfer of ICEs in streptococci (Streptococcus pneumoniae, Streptococcus agalactiae, Streptococcus gordonii, Streptococcus pyogenes), Enterococcus faecalis, and Bacillus subtilis. Conjugative transfer is carried out on solid media and selection of transconjugants is performed with a multilayer plating. This protocol allows the transfer of large genetic elements with a size up to 81 kb, and a transfer frequency up to 6.7 × 10−3 transconjugants/donor cells.
Related Topics
Concepts
Mobile genetic elements
Enterococcus faecalis
Streptococcus gordonii
Biology
Horizontal gene transfer
Genetics
Genome
Bacterial genome size
Streptococcus agalactiae
Enterococcus
Bacillus subtilis
Streptococcus
Microbiology
Computational biology
Gene
Bacteria
Escherichia coli
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3390/mps4030059
- https://www.mdpi.com/2409-9279/4/3/59/pdf?version=1630499268
- OA Status
- gold
- Cited By
- 9
- References
- 17
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3196947700
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W3196947700Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.3390/mps4030059Digital Object Identifier
- Title
-
A Mating Procedure for Genetic Transfer of Integrative and Conjugative Elements (ICEs) of Streptococci and EnterococciWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2021Year of publication
- Publication date
-
2021-08-28Full publication date if available
- Authors
-
Francesco Iannelli, Francesco Santoro, Valeria Fox, Gianni PozziList of authors in order
- Landing page
-
https://doi.org/10.3390/mps4030059Publisher landing page
- PDF URL
-
https://www.mdpi.com/2409-9279/4/3/59/pdf?version=1630499268Direct link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
-
https://www.mdpi.com/2409-9279/4/3/59/pdf?version=1630499268Direct OA link when available
- Concepts
-
Mobile genetic elements, Enterococcus faecalis, Streptococcus gordonii, Biology, Horizontal gene transfer, Genetics, Genome, Bacterial genome size, Streptococcus agalactiae, Enterococcus, Bacillus subtilis, Streptococcus, Microbiology, Computational biology, Gene, Bacteria, Escherichia coliTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
9Total citation count in OpenAlex
- Citations by year (recent)
-
2024: 1, 2023: 4, 2022: 2, 2021: 2Per-year citation counts (last 5 years)
- References (count)
-
17Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
Full payload
| id | https://openalex.org/W3196947700 |
|---|---|
| doi | https://doi.org/10.3390/mps4030059 |
| ids.doi | https://doi.org/10.3390/mps4030059 |
| ids.mag | 3196947700 |
| ids.pmid | https://pubmed.ncbi.nlm.nih.gov/34564305 |
| ids.openalex | https://openalex.org/W3196947700 |
| fwci | 1.68010894 |
| type | article |
| title | A Mating Procedure for Genetic Transfer of Integrative and Conjugative Elements (ICEs) of Streptococci and Enterococci |
| biblio.issue | 3 |
| biblio.volume | 4 |
| biblio.last_page | 59 |
| biblio.first_page | 59 |
| topics[0].id | https://openalex.org/T11551 |
| topics[0].field.id | https://openalex.org/fields/27 |
| topics[0].field.display_name | Medicine |
| topics[0].score | 0.9955999851226807 |
| topics[0].domain.id | https://openalex.org/domains/4 |
| topics[0].domain.display_name | Health Sciences |
| topics[0].subfield.id | https://openalex.org/subfields/2713 |
| topics[0].subfield.display_name | Epidemiology |
| topics[0].display_name | Infective Endocarditis Diagnosis and Management |
| topics[1].id | https://openalex.org/T11048 |
| topics[1].field.id | https://openalex.org/fields/23 |
| topics[1].field.display_name | Environmental Science |
| topics[1].score | 0.9926999807357788 |
| topics[1].domain.id | https://openalex.org/domains/3 |
| topics[1].domain.display_name | Physical Sciences |
| topics[1].subfield.id | https://openalex.org/subfields/2303 |
| topics[1].subfield.display_name | Ecology |
| topics[1].display_name | Bacteriophages and microbial interactions |
| topics[2].id | https://openalex.org/T10195 |
| topics[2].field.id | https://openalex.org/fields/27 |
| topics[2].field.display_name | Medicine |
| topics[2].score | 0.9921000003814697 |
| topics[2].domain.id | https://openalex.org/domains/4 |
| topics[2].domain.display_name | Health Sciences |
| topics[2].subfield.id | https://openalex.org/subfields/2725 |
| topics[2].subfield.display_name | Infectious Diseases |
| topics[2].display_name | Antimicrobial Resistance in Staphylococcus |
| is_xpac | False |
| apc_list.value | 1600 |
| apc_list.currency | CHF |
| apc_list.value_usd | 1732 |
| apc_paid.value | 1600 |
| apc_paid.currency | CHF |
| apc_paid.value_usd | 1732 |
| concepts[0].id | https://openalex.org/C132533263 |
| concepts[0].level | 4 |
| concepts[0].score | 0.8263026475906372 |
| concepts[0].wikidata | https://www.wikidata.org/wiki/Q2347362 |
| concepts[0].display_name | Mobile genetic elements |
| concepts[1].id | https://openalex.org/C2779895986 |
| concepts[1].level | 4 |
| concepts[1].score | 0.7574483156204224 |
| concepts[1].wikidata | https://www.wikidata.org/wiki/Q140014 |
| concepts[1].display_name | Enterococcus faecalis |
| concepts[2].id | https://openalex.org/C2780368718 |
| concepts[2].level | 4 |
| concepts[2].score | 0.7070693969726562 |
| concepts[2].wikidata | https://www.wikidata.org/wiki/Q7623352 |
| concepts[2].display_name | Streptococcus gordonii |
| concepts[3].id | https://openalex.org/C86803240 |
| concepts[3].level | 0 |
| concepts[3].score | 0.6822163462638855 |
| concepts[3].wikidata | https://www.wikidata.org/wiki/Q420 |
| concepts[3].display_name | Biology |
| concepts[4].id | https://openalex.org/C92938381 |
| concepts[4].level | 4 |
| concepts[4].score | 0.6039172410964966 |
| concepts[4].wikidata | https://www.wikidata.org/wiki/Q83185 |
| concepts[4].display_name | Horizontal gene transfer |
| concepts[5].id | https://openalex.org/C54355233 |
| concepts[5].level | 1 |
| concepts[5].score | 0.52858567237854 |
| concepts[5].wikidata | https://www.wikidata.org/wiki/Q7162 |
| concepts[5].display_name | Genetics |
| concepts[6].id | https://openalex.org/C141231307 |
| concepts[6].level | 3 |
| concepts[6].score | 0.5087915062904358 |
| concepts[6].wikidata | https://www.wikidata.org/wiki/Q7020 |
| concepts[6].display_name | Genome |
| concepts[7].id | https://openalex.org/C3742359 |
| concepts[7].level | 4 |
| concepts[7].score | 0.4919159412384033 |
| concepts[7].wikidata | https://www.wikidata.org/wiki/Q4839988 |
| concepts[7].display_name | Bacterial genome size |
| concepts[8].id | https://openalex.org/C2777860557 |
| concepts[8].level | 4 |
| concepts[8].score | 0.4833207130432129 |
| concepts[8].wikidata | https://www.wikidata.org/wiki/Q132949 |
| concepts[8].display_name | Streptococcus agalactiae |
| concepts[9].id | https://openalex.org/C2780585763 |
| concepts[9].level | 3 |
| concepts[9].score | 0.47847339510917664 |
| concepts[9].wikidata | https://www.wikidata.org/wiki/Q517258 |
| concepts[9].display_name | Enterococcus |
| concepts[10].id | https://openalex.org/C2777272437 |
| concepts[10].level | 3 |
| concepts[10].score | 0.4773397743701935 |
| concepts[10].wikidata | https://www.wikidata.org/wiki/Q131238 |
| concepts[10].display_name | Bacillus subtilis |
| concepts[11].id | https://openalex.org/C2778276568 |
| concepts[11].level | 3 |
| concepts[11].score | 0.4444096088409424 |
| concepts[11].wikidata | https://www.wikidata.org/wiki/Q190161 |
| concepts[11].display_name | Streptococcus |
| concepts[12].id | https://openalex.org/C89423630 |
| concepts[12].level | 1 |
| concepts[12].score | 0.4413304030895233 |
| concepts[12].wikidata | https://www.wikidata.org/wiki/Q7193 |
| concepts[12].display_name | Microbiology |
| concepts[13].id | https://openalex.org/C70721500 |
| concepts[13].level | 1 |
| concepts[13].score | 0.366848886013031 |
| concepts[13].wikidata | https://www.wikidata.org/wiki/Q177005 |
| concepts[13].display_name | Computational biology |
| concepts[14].id | https://openalex.org/C104317684 |
| concepts[14].level | 2 |
| concepts[14].score | 0.3075176477432251 |
| concepts[14].wikidata | https://www.wikidata.org/wiki/Q7187 |
| concepts[14].display_name | Gene |
| concepts[15].id | https://openalex.org/C523546767 |
| concepts[15].level | 2 |
| concepts[15].score | 0.2539128363132477 |
| concepts[15].wikidata | https://www.wikidata.org/wiki/Q10876 |
| concepts[15].display_name | Bacteria |
| concepts[16].id | https://openalex.org/C547475151 |
| concepts[16].level | 3 |
| concepts[16].score | 0.1475416123867035 |
| concepts[16].wikidata | https://www.wikidata.org/wiki/Q25419 |
| concepts[16].display_name | Escherichia coli |
| keywords[0].id | https://openalex.org/keywords/mobile-genetic-elements |
| keywords[0].score | 0.8263026475906372 |
| keywords[0].display_name | Mobile genetic elements |
| keywords[1].id | https://openalex.org/keywords/enterococcus-faecalis |
| keywords[1].score | 0.7574483156204224 |
| keywords[1].display_name | Enterococcus faecalis |
| keywords[2].id | https://openalex.org/keywords/streptococcus-gordonii |
| keywords[2].score | 0.7070693969726562 |
| keywords[2].display_name | Streptococcus gordonii |
| keywords[3].id | https://openalex.org/keywords/biology |
| keywords[3].score | 0.6822163462638855 |
| keywords[3].display_name | Biology |
| keywords[4].id | https://openalex.org/keywords/horizontal-gene-transfer |
| keywords[4].score | 0.6039172410964966 |
| keywords[4].display_name | Horizontal gene transfer |
| keywords[5].id | https://openalex.org/keywords/genetics |
| keywords[5].score | 0.52858567237854 |
| keywords[5].display_name | Genetics |
| keywords[6].id | https://openalex.org/keywords/genome |
| keywords[6].score | 0.5087915062904358 |
| keywords[6].display_name | Genome |
| keywords[7].id | https://openalex.org/keywords/bacterial-genome-size |
| keywords[7].score | 0.4919159412384033 |
| keywords[7].display_name | Bacterial genome size |
| keywords[8].id | https://openalex.org/keywords/streptococcus-agalactiae |
| keywords[8].score | 0.4833207130432129 |
| keywords[8].display_name | Streptococcus agalactiae |
| keywords[9].id | https://openalex.org/keywords/enterococcus |
| keywords[9].score | 0.47847339510917664 |
| keywords[9].display_name | Enterococcus |
| keywords[10].id | https://openalex.org/keywords/bacillus-subtilis |
| keywords[10].score | 0.4773397743701935 |
| keywords[10].display_name | Bacillus subtilis |
| keywords[11].id | https://openalex.org/keywords/streptococcus |
| keywords[11].score | 0.4444096088409424 |
| keywords[11].display_name | Streptococcus |
| keywords[12].id | https://openalex.org/keywords/microbiology |
| keywords[12].score | 0.4413304030895233 |
| keywords[12].display_name | Microbiology |
| keywords[13].id | https://openalex.org/keywords/computational-biology |
| keywords[13].score | 0.366848886013031 |
| keywords[13].display_name | Computational biology |
| keywords[14].id | https://openalex.org/keywords/gene |
| keywords[14].score | 0.3075176477432251 |
| keywords[14].display_name | Gene |
| keywords[15].id | https://openalex.org/keywords/bacteria |
| keywords[15].score | 0.2539128363132477 |
| keywords[15].display_name | Bacteria |
| keywords[16].id | https://openalex.org/keywords/escherichia-coli |
| keywords[16].score | 0.1475416123867035 |
| keywords[16].display_name | Escherichia coli |
| language | en |
| locations[0].id | doi:10.3390/mps4030059 |
| locations[0].is_oa | True |
| locations[0].source.id | https://openalex.org/S4210221179 |
| locations[0].source.issn | 2409-9279 |
| locations[0].source.type | journal |
| locations[0].source.is_oa | True |
| locations[0].source.issn_l | 2409-9279 |
| locations[0].source.is_core | True |
| locations[0].source.is_in_doaj | True |
| locations[0].source.display_name | Methods and Protocols |
| locations[0].source.host_organization | https://openalex.org/P4310310987 |
| locations[0].source.host_organization_name | Multidisciplinary Digital Publishing Institute |
| locations[0].source.host_organization_lineage | https://openalex.org/P4310310987 |
| locations[0].license | cc-by |
| locations[0].pdf_url | https://www.mdpi.com/2409-9279/4/3/59/pdf?version=1630499268 |
| locations[0].version | publishedVersion |
| locations[0].raw_type | journal-article |
| locations[0].license_id | https://openalex.org/licenses/cc-by |
| locations[0].is_accepted | True |
| locations[0].is_published | True |
| locations[0].raw_source_name | Methods and Protocols |
| locations[0].landing_page_url | https://doi.org/10.3390/mps4030059 |
| locations[1].id | pmid:34564305 |
| locations[1].is_oa | False |
| locations[1].source.id | https://openalex.org/S4306525036 |
| locations[1].source.issn | |
| locations[1].source.type | repository |
| locations[1].source.is_oa | False |
| locations[1].source.issn_l | |
| locations[1].source.is_core | False |
| locations[1].source.is_in_doaj | False |
| locations[1].source.display_name | PubMed |
| locations[1].source.host_organization | https://openalex.org/I1299303238 |
| locations[1].source.host_organization_name | National Institutes of Health |
| locations[1].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[1].license | |
| locations[1].pdf_url | |
| locations[1].version | publishedVersion |
| locations[1].raw_type | |
| locations[1].license_id | |
| locations[1].is_accepted | True |
| locations[1].is_published | True |
| locations[1].raw_source_name | Methods and protocols |
| locations[1].landing_page_url | https://pubmed.ncbi.nlm.nih.gov/34564305 |
| locations[2].id | pmh:oai:mdpi.com:/2409-9279/4/3/59/ |
| locations[2].is_oa | True |
| locations[2].source.id | https://openalex.org/S4306400947 |
| locations[2].source.issn | |
| locations[2].source.type | repository |
| locations[2].source.is_oa | True |
| locations[2].source.issn_l | |
| locations[2].source.is_core | False |
| locations[2].source.is_in_doaj | False |
| locations[2].source.display_name | MDPI (MDPI AG) |
| locations[2].source.host_organization | https://openalex.org/I4210097602 |
| locations[2].source.host_organization_name | Multidisciplinary Digital Publishing Institute (Switzerland) |
| locations[2].source.host_organization_lineage | https://openalex.org/I4210097602 |
| locations[2].license | cc-by |
| locations[2].pdf_url | |
| locations[2].version | submittedVersion |
| locations[2].raw_type | Text |
| locations[2].license_id | https://openalex.org/licenses/cc-by |
| locations[2].is_accepted | False |
| locations[2].is_published | False |
| locations[2].raw_source_name | Methods and Protocols; Volume 4; Issue 3; Pages: 59 |
| locations[2].landing_page_url | https://dx.doi.org/10.3390/mps4030059 |
| locations[3].id | pmh:oai:europepmc.org:7376343 |
| locations[3].is_oa | True |
| locations[3].source.id | https://openalex.org/S4306400806 |
| locations[3].source.issn | |
| locations[3].source.type | repository |
| locations[3].source.is_oa | False |
| locations[3].source.issn_l | |
| locations[3].source.is_core | False |
| locations[3].source.is_in_doaj | False |
| locations[3].source.display_name | Europe PMC (PubMed Central) |
| locations[3].source.host_organization | https://openalex.org/I1303153112 |
| locations[3].source.host_organization_name | European Bioinformatics Institute |
| locations[3].source.host_organization_lineage | https://openalex.org/I1303153112 |
| locations[3].license | other-oa |
| locations[3].pdf_url | |
| locations[3].version | submittedVersion |
| locations[3].raw_type | Text |
| locations[3].license_id | https://openalex.org/licenses/other-oa |
| locations[3].is_accepted | False |
| locations[3].is_published | False |
| locations[3].raw_source_name | |
| locations[3].landing_page_url | http://europepmc.org/pmc/articles/PMC8482134 |
| locations[4].id | pmh:oai:doaj.org/article:f80ac07e29f14fffadb5bd5d31d3c5e2 |
| locations[4].is_oa | True |
| locations[4].source.id | https://openalex.org/S4306401280 |
| locations[4].source.issn | |
| locations[4].source.type | repository |
| locations[4].source.is_oa | False |
| locations[4].source.issn_l | |
| locations[4].source.is_core | False |
| locations[4].source.is_in_doaj | False |
| locations[4].source.display_name | DOAJ (DOAJ: Directory of Open Access Journals) |
| locations[4].source.host_organization | |
| locations[4].source.host_organization_name | |
| locations[4].source.host_organization_lineage | |
| locations[4].license | cc-by-sa |
| locations[4].pdf_url | |
| locations[4].version | submittedVersion |
| locations[4].raw_type | article |
| locations[4].license_id | https://openalex.org/licenses/cc-by-sa |
| locations[4].is_accepted | False |
| locations[4].is_published | False |
| locations[4].raw_source_name | Methods and Protocols, Vol 4, Iss 3, p 59 (2021) |
| locations[4].landing_page_url | https://doaj.org/article/f80ac07e29f14fffadb5bd5d31d3c5e2 |
| locations[5].id | pmh:oai:pubmedcentral.nih.gov:8482134 |
| locations[5].is_oa | True |
| locations[5].source.id | https://openalex.org/S2764455111 |
| locations[5].source.issn | |
| locations[5].source.type | repository |
| locations[5].source.is_oa | False |
| locations[5].source.issn_l | |
| locations[5].source.is_core | False |
| locations[5].source.is_in_doaj | False |
| locations[5].source.display_name | PubMed Central |
| locations[5].source.host_organization | https://openalex.org/I1299303238 |
| locations[5].source.host_organization_name | National Institutes of Health |
| locations[5].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[5].license | other-oa |
| locations[5].pdf_url | |
| locations[5].version | submittedVersion |
| locations[5].raw_type | Text |
| locations[5].license_id | https://openalex.org/licenses/other-oa |
| locations[5].is_accepted | False |
| locations[5].is_published | False |
| locations[5].raw_source_name | Methods Protoc |
| locations[5].landing_page_url | https://www.ncbi.nlm.nih.gov/pmc/articles/8482134 |
| locations[6].id | pmh:oai:usiena-air.unisi.it:11365/1168965 |
| locations[6].is_oa | True |
| locations[6].source.id | https://openalex.org/S4377196319 |
| locations[6].source.issn | |
| locations[6].source.type | repository |
| locations[6].source.is_oa | False |
| locations[6].source.issn_l | |
| locations[6].source.is_core | False |
| locations[6].source.is_in_doaj | False |
| locations[6].source.display_name | Use Siena air (University of Siena) |
| locations[6].source.host_organization | https://openalex.org/I102064193 |
| locations[6].source.host_organization_name | University of Siena |
| locations[6].source.host_organization_lineage | https://openalex.org/I102064193 |
| locations[6].license | cc-by |
| locations[6].pdf_url | |
| locations[6].version | submittedVersion |
| locations[6].raw_type | info:eu-repo/semantics/article |
| locations[6].license_id | https://openalex.org/licenses/cc-by |
| locations[6].is_accepted | False |
| locations[6].is_published | False |
| locations[6].raw_source_name | |
| locations[6].landing_page_url | https://www.mdpi.com/2409-9279/4/3/59 |
| indexed_in | crossref, doaj, pubmed |
| authorships[0].author.id | https://openalex.org/A5010763200 |
| authorships[0].author.orcid | https://orcid.org/0000-0001-5064-1496 |
| authorships[0].author.display_name | Francesco Iannelli |
| authorships[0].countries | IT |
| authorships[0].affiliations[0].institution_ids | https://openalex.org/I102064193 |
| authorships[0].affiliations[0].raw_affiliation_string | Laboratory of Molecular Microbiology and Biotechnology, Department of Medical Biotechnologies, University of Siena, 53100 Siena, Italy |
| authorships[0].institutions[0].id | https://openalex.org/I102064193 |
| authorships[0].institutions[0].ror | https://ror.org/01tevnk56 |
| authorships[0].institutions[0].type | education |
| authorships[0].institutions[0].lineage | https://openalex.org/I102064193 |
| authorships[0].institutions[0].country_code | IT |
| authorships[0].institutions[0].display_name | University of Siena |
| authorships[0].author_position | first |
| authorships[0].raw_author_name | Francesco Iannelli |
| authorships[0].is_corresponding | True |
| authorships[0].raw_affiliation_strings | Laboratory of Molecular Microbiology and Biotechnology, Department of Medical Biotechnologies, University of Siena, 53100 Siena, Italy |
| authorships[1].author.id | https://openalex.org/A5069089383 |
| authorships[1].author.orcid | https://orcid.org/0000-0003-3052-3518 |
| authorships[1].author.display_name | Francesco Santoro |
| authorships[1].countries | IT |
| authorships[1].affiliations[0].institution_ids | https://openalex.org/I102064193 |
| authorships[1].affiliations[0].raw_affiliation_string | Laboratory of Molecular Microbiology and Biotechnology, Department of Medical Biotechnologies, University of Siena, 53100 Siena, Italy |
| authorships[1].institutions[0].id | https://openalex.org/I102064193 |
| authorships[1].institutions[0].ror | https://ror.org/01tevnk56 |
| authorships[1].institutions[0].type | education |
| authorships[1].institutions[0].lineage | https://openalex.org/I102064193 |
| authorships[1].institutions[0].country_code | IT |
| authorships[1].institutions[0].display_name | University of Siena |
| authorships[1].author_position | middle |
| authorships[1].raw_author_name | Francesco Santoro |
| authorships[1].is_corresponding | False |
| authorships[1].raw_affiliation_strings | Laboratory of Molecular Microbiology and Biotechnology, Department of Medical Biotechnologies, University of Siena, 53100 Siena, Italy |
| authorships[2].author.id | https://openalex.org/A5047440120 |
| authorships[2].author.orcid | https://orcid.org/0000-0002-4552-8461 |
| authorships[2].author.display_name | Valeria Fox |
| authorships[2].countries | IT |
| authorships[2].affiliations[0].institution_ids | https://openalex.org/I102064193 |
| authorships[2].affiliations[0].raw_affiliation_string | Laboratory of Molecular Microbiology and Biotechnology, Department of Medical Biotechnologies, University of Siena, 53100 Siena, Italy |
| authorships[2].institutions[0].id | https://openalex.org/I102064193 |
| authorships[2].institutions[0].ror | https://ror.org/01tevnk56 |
| authorships[2].institutions[0].type | education |
| authorships[2].institutions[0].lineage | https://openalex.org/I102064193 |
| authorships[2].institutions[0].country_code | IT |
| authorships[2].institutions[0].display_name | University of Siena |
| authorships[2].author_position | middle |
| authorships[2].raw_author_name | Valeria Fox |
| authorships[2].is_corresponding | False |
| authorships[2].raw_affiliation_strings | Laboratory of Molecular Microbiology and Biotechnology, Department of Medical Biotechnologies, University of Siena, 53100 Siena, Italy |
| authorships[3].author.id | https://openalex.org/A5064694313 |
| authorships[3].author.orcid | https://orcid.org/0000-0002-9557-4714 |
| authorships[3].author.display_name | Gianni Pozzi |
| authorships[3].countries | IT |
| authorships[3].affiliations[0].institution_ids | https://openalex.org/I102064193 |
| authorships[3].affiliations[0].raw_affiliation_string | Laboratory of Molecular Microbiology and Biotechnology, Department of Medical Biotechnologies, University of Siena, 53100 Siena, Italy |
| authorships[3].institutions[0].id | https://openalex.org/I102064193 |
| authorships[3].institutions[0].ror | https://ror.org/01tevnk56 |
| authorships[3].institutions[0].type | education |
| authorships[3].institutions[0].lineage | https://openalex.org/I102064193 |
| authorships[3].institutions[0].country_code | IT |
| authorships[3].institutions[0].display_name | University of Siena |
| authorships[3].author_position | last |
| authorships[3].raw_author_name | Gianni Pozzi |
| authorships[3].is_corresponding | False |
| authorships[3].raw_affiliation_strings | Laboratory of Molecular Microbiology and Biotechnology, Department of Medical Biotechnologies, University of Siena, 53100 Siena, Italy |
| has_content.pdf | True |
| has_content.grobid_xml | True |
| is_paratext | False |
| open_access.is_oa | True |
| open_access.oa_url | https://www.mdpi.com/2409-9279/4/3/59/pdf?version=1630499268 |
| open_access.oa_status | gold |
| open_access.any_repository_has_fulltext | False |
| created_date | 2025-10-10T00:00:00 |
| display_name | A Mating Procedure for Genetic Transfer of Integrative and Conjugative Elements (ICEs) of Streptococci and Enterococci |
| has_fulltext | True |
| is_retracted | False |
| updated_date | 2025-11-06T03:46:38.306776 |
| primary_topic.id | https://openalex.org/T11551 |
| primary_topic.field.id | https://openalex.org/fields/27 |
| primary_topic.field.display_name | Medicine |
| primary_topic.score | 0.9955999851226807 |
| primary_topic.domain.id | https://openalex.org/domains/4 |
| primary_topic.domain.display_name | Health Sciences |
| primary_topic.subfield.id | https://openalex.org/subfields/2713 |
| primary_topic.subfield.display_name | Epidemiology |
| primary_topic.display_name | Infective Endocarditis Diagnosis and Management |
| related_works | https://openalex.org/W1989898630, https://openalex.org/W4390909895, https://openalex.org/W2945663299, https://openalex.org/W1891155455, https://openalex.org/W1498144456, https://openalex.org/W2520150954, https://openalex.org/W2959951739, https://openalex.org/W3154851329, https://openalex.org/W2983142016, https://openalex.org/W2108332051 |
| cited_by_count | 9 |
| counts_by_year[0].year | 2024 |
| counts_by_year[0].cited_by_count | 1 |
| counts_by_year[1].year | 2023 |
| counts_by_year[1].cited_by_count | 4 |
| counts_by_year[2].year | 2022 |
| counts_by_year[2].cited_by_count | 2 |
| counts_by_year[3].year | 2021 |
| counts_by_year[3].cited_by_count | 2 |
| locations_count | 7 |
| best_oa_location.id | doi:10.3390/mps4030059 |
| best_oa_location.is_oa | True |
| best_oa_location.source.id | https://openalex.org/S4210221179 |
| best_oa_location.source.issn | 2409-9279 |
| best_oa_location.source.type | journal |
| best_oa_location.source.is_oa | True |
| best_oa_location.source.issn_l | 2409-9279 |
| best_oa_location.source.is_core | True |
| best_oa_location.source.is_in_doaj | True |
| best_oa_location.source.display_name | Methods and Protocols |
| best_oa_location.source.host_organization | https://openalex.org/P4310310987 |
| best_oa_location.source.host_organization_name | Multidisciplinary Digital Publishing Institute |
| best_oa_location.source.host_organization_lineage | https://openalex.org/P4310310987 |
| best_oa_location.license | cc-by |
| best_oa_location.pdf_url | https://www.mdpi.com/2409-9279/4/3/59/pdf?version=1630499268 |
| best_oa_location.version | publishedVersion |
| best_oa_location.raw_type | journal-article |
| best_oa_location.license_id | https://openalex.org/licenses/cc-by |
| best_oa_location.is_accepted | True |
| best_oa_location.is_published | True |
| best_oa_location.raw_source_name | Methods and Protocols |
| best_oa_location.landing_page_url | https://doi.org/10.3390/mps4030059 |
| primary_location.id | doi:10.3390/mps4030059 |
| primary_location.is_oa | True |
| primary_location.source.id | https://openalex.org/S4210221179 |
| primary_location.source.issn | 2409-9279 |
| primary_location.source.type | journal |
| primary_location.source.is_oa | True |
| primary_location.source.issn_l | 2409-9279 |
| primary_location.source.is_core | True |
| primary_location.source.is_in_doaj | True |
| primary_location.source.display_name | Methods and Protocols |
| primary_location.source.host_organization | https://openalex.org/P4310310987 |
| primary_location.source.host_organization_name | Multidisciplinary Digital Publishing Institute |
| primary_location.source.host_organization_lineage | https://openalex.org/P4310310987 |
| primary_location.license | cc-by |
| primary_location.pdf_url | https://www.mdpi.com/2409-9279/4/3/59/pdf?version=1630499268 |
| primary_location.version | publishedVersion |
| primary_location.raw_type | journal-article |
| primary_location.license_id | https://openalex.org/licenses/cc-by |
| primary_location.is_accepted | True |
| primary_location.is_published | True |
| primary_location.raw_source_name | Methods and Protocols |
| primary_location.landing_page_url | https://doi.org/10.3390/mps4030059 |
| publication_date | 2021-08-28 |
| publication_year | 2021 |
| referenced_works | https://openalex.org/W2108332051, https://openalex.org/W2027831845, https://openalex.org/W2946318463, https://openalex.org/W1832517154, https://openalex.org/W2885104189, https://openalex.org/W2152087501, https://openalex.org/W2007321059, https://openalex.org/W2261944450, https://openalex.org/W2771336639, https://openalex.org/W2051068292, https://openalex.org/W2154940506, https://openalex.org/W2868353123, https://openalex.org/W1991722426, https://openalex.org/W2001945908, https://openalex.org/W1969071924, https://openalex.org/W2048072405, https://openalex.org/W1550742594 |
| referenced_works_count | 17 |
| abstract_inverted_index.a | 64, 95, 148, 161, 168 |
| abstract_inverted_index.81 | 165 |
| abstract_inverted_index.In | 85 |
| abstract_inverted_index.an | 104 |
| abstract_inverted_index.as | 17, 19 |
| abstract_inverted_index.in | 112, 118 |
| abstract_inverted_index.is | 41, 63, 135, 145 |
| abstract_inverted_index.of | 2, 12, 21, 29, 35, 54, 58, 66, 74, 78, 98, 116, 143, 156 |
| abstract_inverted_index.on | 138 |
| abstract_inverted_index.or | 81 |
| abstract_inverted_index.to | 50, 164, 172 |
| abstract_inverted_index.up | 163, 171 |
| abstract_inverted_index.we | 102 |
| abstract_inverted_index.× | 174 |
| abstract_inverted_index.25% | 20 |
| abstract_inverted_index.6.7 | 173 |
| abstract_inverted_index.DNA | 0 |
| abstract_inverted_index.and | 87, 90, 106, 130, 141, 167 |
| abstract_inverted_index.are | 48 |
| abstract_inverted_index.for | 111 |
| abstract_inverted_index.has | 6 |
| abstract_inverted_index.kb, | 166 |
| abstract_inverted_index.out | 137 |
| abstract_inverted_index.set | 11 |
| abstract_inverted_index.the | 9, 22, 26, 32, 36, 52, 59, 72, 79, 99, 154 |
| abstract_inverted_index.ICEs | 117 |
| abstract_inverted_index.MGEs | 75 |
| abstract_inverted_index.This | 151 |
| abstract_inverted_index.gene | 68 |
| abstract_inverted_index.huge | 27 |
| abstract_inverted_index.much | 18 |
| abstract_inverted_index.part | 57, 97 |
| abstract_inverted_index.same | 80 |
| abstract_inverted_index.size | 162 |
| abstract_inverted_index.that | 8 |
| abstract_inverted_index.this | 55 |
| abstract_inverted_index.with | 147, 160 |
| abstract_inverted_index.Here, | 101 |
| abstract_inverted_index.among | 76 |
| abstract_inverted_index.data, | 31 |
| abstract_inverted_index.genes | 14 |
| abstract_inverted_index.large | 96, 157 |
| abstract_inverted_index.media | 140 |
| abstract_inverted_index.plate | 108 |
| abstract_inverted_index.solid | 139 |
| abstract_inverted_index.vitro | 113 |
| abstract_inverted_index.which | 70 |
| abstract_inverted_index.whole | 3 |
| abstract_inverted_index.(ICEs) | 93 |
| abstract_inverted_index.(MGEs) | 40 |
| abstract_inverted_index.10−3 | 175 |
| abstract_inverted_index.allows | 71, 153 |
| abstract_inverted_index.cells. | 177 |
| abstract_inverted_index.entire | 10 |
| abstract_inverted_index.mating | 109 |
| abstract_inverted_index.mobile | 13, 37 |
| abstract_inverted_index.needed | 49 |
| abstract_inverted_index.rarely | 42 |
| abstract_inverted_index.Despite | 25 |
| abstract_inverted_index.biology | 53 |
| abstract_inverted_index.carried | 136 |
| abstract_inverted_index.genetic | 38, 158 |
| abstract_inverted_index.genome. | 24, 61 |
| abstract_inverted_index.genomes | 5 |
| abstract_inverted_index.strains | 77 |
| abstract_inverted_index.Bacillus | 131 |
| abstract_inverted_index.analysis | 34 |
| abstract_inverted_index.describe | 103 |
| abstract_inverted_index.elements | 39, 92, 159 |
| abstract_inverted_index.exchange | 73 |
| abstract_inverted_index.plating. | 150 |
| abstract_inverted_index.protocol | 110, 152 |
| abstract_inverted_index.revealed | 7 |
| abstract_inverted_index.sequence | 30 |
| abstract_inverted_index.species. | 84 |
| abstract_inverted_index.transfer | 69, 115, 134, 155, 169 |
| abstract_inverted_index.bacterial | 4, 23, 60, 83 |
| abstract_inverted_index.different | 82 |
| abstract_inverted_index.efficient | 105 |
| abstract_inverted_index.faecalis, | 129 |
| abstract_inverted_index.frequency | 170 |
| abstract_inverted_index.gordonii, | 125 |
| abstract_inverted_index.important | 56 |
| abstract_inverted_index.mechanism | 65 |
| abstract_inverted_index.mobilome. | 100 |
| abstract_inverted_index.performed | 146 |
| abstract_inverted_index.protocols | 47 |
| abstract_inverted_index.reported. | 43 |
| abstract_inverted_index.represent | 94 |
| abstract_inverted_index.selection | 142 |
| abstract_inverted_index.subtilis. | 132 |
| abstract_inverted_index.(mobilome) | 15 |
| abstract_inverted_index.Therefore, | 44 |
| abstract_inverted_index.functional | 33 |
| abstract_inverted_index.horizontal | 67 |
| abstract_inverted_index.laboratory | 46 |
| abstract_inverted_index.multilayer | 149 |
| abstract_inverted_index.pyogenes), | 127 |
| abstract_inverted_index.represents | 16 |
| abstract_inverted_index.sequencing | 1 |
| abstract_inverted_index.Conjugation | 62 |
| abstract_inverted_index.Conjugative | 133 |
| abstract_inverted_index.agalactiae, | 123 |
| abstract_inverted_index.conjugative | 91, 114 |
| abstract_inverted_index.established | 45 |
| abstract_inverted_index.integrative | 89 |
| abstract_inverted_index.investigate | 51 |
| abstract_inverted_index.pneumoniae, | 121 |
| abstract_inverted_index.Enterococcus | 128 |
| abstract_inverted_index.availability | 28 |
| abstract_inverted_index.enterococci, | 88 |
| abstract_inverted_index.streptococci | 86, 119 |
| abstract_inverted_index.Streptococcus | 122, 124, 126 |
| abstract_inverted_index.(Streptococcus | 120 |
| abstract_inverted_index.easy-to-perform | 107 |
| abstract_inverted_index.transconjugants | 144 |
| abstract_inverted_index.transconjugants/donor | 176 |
| cited_by_percentile_year.max | 97 |
| cited_by_percentile_year.min | 90 |
| corresponding_author_ids | https://openalex.org/A5010763200 |
| countries_distinct_count | 1 |
| institutions_distinct_count | 4 |
| corresponding_institution_ids | https://openalex.org/I102064193 |
| sustainable_development_goals[0].id | https://metadata.un.org/sdg/15 |
| sustainable_development_goals[0].score | 0.46000000834465027 |
| sustainable_development_goals[0].display_name | Life in Land |
| citation_normalized_percentile.value | 0.81553406 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |