A method for the efficient iron-labeling of patient-derived xenograft cells and cellular imaging validation Article Swipe
Natasha N. Knier
,
Veronica P. Dubois
,
Yuanxin Chen
,
John A. Ronald
,
Paula J. Foster
·
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.14440/jbm.2021.356
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.14440/jbm.2021.356
There is momentum towards implementing patient-derived xenograft models (PDX) in cancer research to reflect the histopathology, tumor behavior, and metastatic properties observed in the original tumor. These models are more predictive of clinical outcomes and are superior to cell lines for preclinical drug evaluation and therapeutic strategies. To study PDX cells preclinically, we used both bioluminescence imaging (BLI) to evaluate cell viability and magnetic particle imaging (MPI), an emerging imaging technology to allow for detection and quantification of iron nanoparticles. The goal of this study was to develop the first successful iron labeling method of breast cancer cells derived from patient brain metastases and validate this method with imaging during tumor development. Luciferase expressing human breast cancer PDX cells (F2-7) were successfully labeled after incubation with micron-sized iron oxide particles (MPIO; 25 μg Fe/ml). NOD/SCID/ILIIrg−/− (n = 5) mice received injections of 1x106 iron-labeled F2-7 cells into the fourth mammary fat pad (MFP). BLI was performed longitudinally to day 49 and MPI was performed up to day 28. In vivo BLI revealed that signal increased over time with tumor development. MPI revealed decreasing signal in the tumor over time. Here, we demonstrate the first application of MPI to monitor the growth of a PDX MFP tumor. To accomplish this, we also demonstrate the first successful labeling of PDX cells with iron oxide particles. Imaging of PDX cells provides a powerful system to better develop personalized therapies targeting breast cancer brain metastasis.
Related Topics
Concepts
Bioluminescence imaging
Iron oxide nanoparticles
Cancer research
Breast cancer
In vivo
Magnetic particle imaging
Cancer cell
Metastasis
Molecular imaging
Cancer
Medicine
Human breast
Preclinical imaging
Chemistry
Pathology
Luciferase
Internal medicine
Iron oxide
Biology
Nanotechnology
Nanoparticle
Magnetic nanoparticles
Materials science
Gene
Biotechnology
Transfection
Organic chemistry
Biochemistry
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.14440/jbm.2021.356
- https://polscientific.com/jbm/index.php/jbm/article/download/356/434
- OA Status
- diamond
- Cited By
- 2
- References
- 32
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3197755089
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W3197755089Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.14440/jbm.2021.356Digital Object Identifier
- Title
-
A method for the efficient iron-labeling of patient-derived xenograft cells and cellular imaging validationWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2021Year of publication
- Publication date
-
2021-09-02Full publication date if available
- Authors
-
Natasha N. Knier, Veronica P. Dubois, Yuanxin Chen, John A. Ronald, Paula J. FosterList of authors in order
- Landing page
-
https://doi.org/10.14440/jbm.2021.356Publisher landing page
- PDF URL
-
https://polscientific.com/jbm/index.php/jbm/article/download/356/434Direct link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
diamondOpen access status per OpenAlex
- OA URL
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https://polscientific.com/jbm/index.php/jbm/article/download/356/434Direct OA link when available
- Concepts
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Bioluminescence imaging, Iron oxide nanoparticles, Cancer research, Breast cancer, In vivo, Magnetic particle imaging, Cancer cell, Metastasis, Molecular imaging, Cancer, Medicine, Human breast, Preclinical imaging, Chemistry, Pathology, Luciferase, Internal medicine, Iron oxide, Biology, Nanotechnology, Nanoparticle, Magnetic nanoparticles, Materials science, Gene, Biotechnology, Transfection, Organic chemistry, BiochemistryTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
2Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 1, 2021: 1Per-year citation counts (last 5 years)
- References (count)
-
32Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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