A novel immuno-molecular strategy for the detection of Streptococcus pneumoniae serotypes in human cerebrospinal and middle ear fluids Article Swipe
Gowrisankar Rajam
,
Kate L. Hicks
,
Joseph M. Antonello
,
Yuhua Zhang
,
Sarah Irey
,
Julia Kelly
,
Stephanie Cooper
,
Leonard J. Rubinstein
·
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.1016/j.jim.2023.113516
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.1016/j.jim.2023.113516
Streptococcus pneumoniae is one of the most common microorganisms causing acute otitis media (AOM) in children. While bacterial culture of middle ear fluid (MEF) is the gold standard to detect the etiological organisms, several host and pathogen factors impact the survival of the organisms resulting in false negatives. To overcome this limitation, we have developed and validated an innovative multiplex immuno-molecular assay to screen and detect the S. pneumoniae 15-valent pneumococcal conjugate vaccine (PCV15; STs 1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F, 22F, 23F, and 33F) vaccine serotypes in MEF. This novel in vitro approach involves two-step testing. First, the MEF specimens were tested for highly conserved pneumococcal genes, autolysin, lytA, and pneumolysin, ply using direct PCR to identify pneumococcus positive specimens. The pneumococcus positive specimens were screened for the presence of vaccine serotype specific pneumococcal polysaccharides using a 15-plex Pneumococcal Antigen Detection (PAD) assay, with specific capture and detection monoclonal antibodies. Due to the lack of availability of MEF samples, cerebrospinal fluid (CSF) was used as the surrogate matrix for the development and validation of the PCR-PAD assays. The PCR and PAD assays were separately evaluated for sensitivity and specificity. Subsequently, the PCR-PAD assays were cross-validated with human MEF samples (n = 39) which were culture confirmed to contain relevant bacterial strains. The combined PCR-PAD assays demonstrated high rate of agreement 94.9% (95% CI; 82.7, 99.4%) with historical Quellung serotype data of these MEF samples. This novel PCR-PAD assay demonstrates the feasibility of combining molecular and immunological assays to screen and identify PCV15 pneumococcal vaccine serotypes in AOM clinical samples.
Related Topics
Concepts
Streptococcus pneumoniae
Microbiology
Pneumolysin
Serotype
Biology
Pneumococcal infections
Multiplex polymerase chain reaction
Antigen
Monoclonal antibody
Pathogen
Virology
Antibody
Polymerase chain reaction
Immunology
Antibiotics
Gene
Biochemistry
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1016/j.jim.2023.113516
- OA Status
- hybrid
- Cited By
- 2
- References
- 28
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4381325759
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4381325759Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.1016/j.jim.2023.113516Digital Object Identifier
- Title
-
A novel immuno-molecular strategy for the detection of Streptococcus pneumoniae serotypes in human cerebrospinal and middle ear fluidsWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2023Year of publication
- Publication date
-
2023-06-20Full publication date if available
- Authors
-
Gowrisankar Rajam, Kate L. Hicks, Joseph M. Antonello, Yuhua Zhang, Sarah Irey, Julia Kelly, Stephanie Cooper, Leonard J. RubinsteinList of authors in order
- Landing page
-
https://doi.org/10.1016/j.jim.2023.113516Publisher landing page
- Open access
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YesWhether a free full text is available
- OA status
-
hybridOpen access status per OpenAlex
- OA URL
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https://doi.org/10.1016/j.jim.2023.113516Direct OA link when available
- Concepts
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Streptococcus pneumoniae, Microbiology, Pneumolysin, Serotype, Biology, Pneumococcal infections, Multiplex polymerase chain reaction, Antigen, Monoclonal antibody, Pathogen, Virology, Antibody, Polymerase chain reaction, Immunology, Antibiotics, Gene, BiochemistryTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
2Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 1, 2024: 1Per-year citation counts (last 5 years)
- References (count)
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28Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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