A Rapid Detection of Haemophilus influenzae Using Multiple Cross Displacement Amplification Linked With Nanoparticle-Based Lateral Flow Biosensor Article Swipe
Qilong Cao
,
Shaoshuai Liang
,
Lin Wang
,
Jun Cao
,
Mengyang Liu
,
Shengpeng Li
,
Xiaolong Cao
,
Yan Guo
·
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.3389/fcimb.2021.721547
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.3389/fcimb.2021.721547
Haemophilus influenzae is a major human pathogenic bacterium, resulting in a series of diseases, such as pneumonia, bacteremia, meningitis. However, it is hard to diagnose H . influenzae quickly. In this study, the multiple cross displacement amplification (MCDA) and nanoparticle-based lateral flow biosensor (LFB) (MCDA-LFB) were combined to detect H . influenzae , which has been proven to be reliable, rapid, and not complicated. On the basis of H . influenzae outer membrane protein P6 gene, 10 specific primers were designed. The best MCDA condition was 61°C for 1 h. The sensitivity of H . influenzae -MCD-LFB assay showed, in the pure cultures, the minimum concentration of genomic DNA templates was 100 fg. The specificity of H . influenzae -MCD-LFB assay showed only H . influenzae templates were detected, and no cross-reactivity was found in non- H . influenzae isolates and other Haemophilus species. In 56 sputum samples, with MCDA-LFB method and PCR detection, 21 samples were positive, which was in consistent with the traditional culture method. The accuracy of diagnosis of MCDA-LFB, in comparison with the traditional culture method and PCR detection, can reach 100%, indicating that the MCDA-LFB assay gains an advantage over the cultured-based method for target pathogen detection. In conclusion, the MCDA-LFB assay is suitable for the sensitive, rapid, and specific detection of H . influenzae , which might be used as a potential diagnostic tool for H . influenzae in basic and clinical laboratories.
Related Topics
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3389/fcimb.2021.721547
- OA Status
- gold
- Cited By
- 9
- References
- 29
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3201010402
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W3201010402Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.3389/fcimb.2021.721547Digital Object Identifier
- Title
-
A Rapid Detection of Haemophilus influenzae Using Multiple Cross Displacement Amplification Linked With Nanoparticle-Based Lateral Flow BiosensorWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2021Year of publication
- Publication date
-
2021-09-22Full publication date if available
- Authors
-
Qilong Cao, Shaoshuai Liang, Lin Wang, Jun Cao, Mengyang Liu, Shengpeng Li, Xiaolong Cao, Yan GuoList of authors in order
- Landing page
-
https://doi.org/10.3389/fcimb.2021.721547Publisher landing page
- Open access
-
YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
-
https://doi.org/10.3389/fcimb.2021.721547Direct OA link when available
- Concepts
-
Haemophilus influenzae, Microbiology, Biology, AntibioticsTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
9Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 1, 2024: 5, 2022: 2, 2021: 1Per-year citation counts (last 5 years)
- References (count)
-
29Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
Full payload
| id | https://openalex.org/W3201010402 |
|---|---|
| doi | https://doi.org/10.3389/fcimb.2021.721547 |
| ids.doi | https://doi.org/10.3389/fcimb.2021.721547 |
| ids.mag | 3201010402 |
| ids.pmid | https://pubmed.ncbi.nlm.nih.gov/34631602 |
| ids.openalex | https://openalex.org/W3201010402 |
| fwci | 1.34106845 |
| mesh[0].qualifier_ui | |
| mesh[0].descriptor_ui | D015374 |
| mesh[0].is_major_topic | True |
| mesh[0].qualifier_name | |
| mesh[0].descriptor_name | Biosensing Techniques |
| mesh[1].qualifier_ui | Q000235 |
| mesh[1].descriptor_ui | D006193 |
| mesh[1].is_major_topic | False |
| mesh[1].qualifier_name | genetics |
| mesh[1].descriptor_name | Haemophilus influenzae |
| mesh[2].qualifier_ui | |
| mesh[2].descriptor_ui | D006801 |
| mesh[2].is_major_topic | False |
| mesh[2].qualifier_name | |
| mesh[2].descriptor_name | Humans |
| mesh[3].qualifier_ui | |
| mesh[3].descriptor_ui | D053758 |
| mesh[3].is_major_topic | True |
| mesh[3].qualifier_name | |
| mesh[3].descriptor_name | Nanoparticles |
| mesh[4].qualifier_ui | |
| mesh[4].descriptor_ui | D021141 |
| mesh[4].is_major_topic | False |
| mesh[4].qualifier_name | |
| mesh[4].descriptor_name | Nucleic Acid Amplification Techniques |
| mesh[5].qualifier_ui | |
| mesh[5].descriptor_ui | D012680 |
| mesh[5].is_major_topic | False |
| mesh[5].qualifier_name | |
| mesh[5].descriptor_name | Sensitivity and Specificity |
| mesh[6].qualifier_ui | |
| mesh[6].descriptor_ui | D013696 |
| mesh[6].is_major_topic | False |
| mesh[6].qualifier_name | |
| mesh[6].descriptor_name | Temperature |
| mesh[7].qualifier_ui | |
| mesh[7].descriptor_ui | D015374 |
| mesh[7].is_major_topic | True |
| mesh[7].qualifier_name | |
| mesh[7].descriptor_name | Biosensing Techniques |
| mesh[8].qualifier_ui | Q000235 |
| mesh[8].descriptor_ui | D006193 |
| mesh[8].is_major_topic | False |
| mesh[8].qualifier_name | genetics |
| mesh[8].descriptor_name | Haemophilus influenzae |
| mesh[9].qualifier_ui | |
| mesh[9].descriptor_ui | D006801 |
| mesh[9].is_major_topic | False |
| mesh[9].qualifier_name | |
| mesh[9].descriptor_name | Humans |
| mesh[10].qualifier_ui | |
| mesh[10].descriptor_ui | D053758 |
| mesh[10].is_major_topic | True |
| mesh[10].qualifier_name | |
| mesh[10].descriptor_name | Nanoparticles |
| mesh[11].qualifier_ui | |
| mesh[11].descriptor_ui | D021141 |
| mesh[11].is_major_topic | False |
| mesh[11].qualifier_name | |
| mesh[11].descriptor_name | Nucleic Acid Amplification Techniques |
| mesh[12].qualifier_ui | |
| mesh[12].descriptor_ui | D012680 |
| mesh[12].is_major_topic | False |
| mesh[12].qualifier_name | |
| mesh[12].descriptor_name | Sensitivity and Specificity |
| mesh[13].qualifier_ui | |
| mesh[13].descriptor_ui | D013696 |
| mesh[13].is_major_topic | False |
| mesh[13].qualifier_name | |
| mesh[13].descriptor_name | Temperature |
| type | article |
| title | A Rapid Detection of Haemophilus influenzae Using Multiple Cross Displacement Amplification Linked With Nanoparticle-Based Lateral Flow Biosensor |
| biblio.issue | |
| biblio.volume | 11 |
| biblio.last_page | 721547 |
| biblio.first_page | 721547 |
| topics[0].id | https://openalex.org/T10873 |
| topics[0].field.id | https://openalex.org/fields/24 |
| topics[0].field.display_name | Immunology and Microbiology |
| topics[0].score | 0.9998000264167786 |
| topics[0].domain.id | https://openalex.org/domains/1 |
| topics[0].domain.display_name | Life Sciences |
| topics[0].subfield.id | https://openalex.org/subfields/2404 |
| topics[0].subfield.display_name | Microbiology |
| topics[0].display_name | Bacterial Infections and Vaccines |
| topics[1].id | https://openalex.org/T11393 |
| topics[1].field.id | https://openalex.org/fields/22 |
| topics[1].field.display_name | Engineering |
| topics[1].score | 0.9995999932289124 |
| topics[1].domain.id | https://openalex.org/domains/3 |
| topics[1].domain.display_name | Physical Sciences |
| topics[1].subfield.id | https://openalex.org/subfields/2204 |
| topics[1].subfield.display_name | Biomedical Engineering |
| topics[1].display_name | Biosensors and Analytical Detection |
| topics[2].id | https://openalex.org/T10207 |
| topics[2].field.id | https://openalex.org/fields/13 |
| topics[2].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[2].score | 0.9958000183105469 |
| topics[2].domain.id | https://openalex.org/domains/1 |
| topics[2].domain.display_name | Life Sciences |
| topics[2].subfield.id | https://openalex.org/subfields/1312 |
| topics[2].subfield.display_name | Molecular Biology |
| topics[2].display_name | Advanced biosensing and bioanalysis techniques |
| is_xpac | False |
| apc_list.value | 2950 |
| apc_list.currency | USD |
| apc_list.value_usd | 2950 |
| apc_paid.value | 2950 |
| apc_paid.currency | USD |
| apc_paid.value_usd | 2950 |
| concepts[0].id | https://openalex.org/C2776925733 |
| concepts[0].level | 3 |
| concepts[0].score | 0.9404406547546387 |
| concepts[0].wikidata | https://www.wikidata.org/wiki/Q1141979 |
| concepts[0].display_name | Haemophilus influenzae |
| concepts[1].id | https://openalex.org/C89423630 |
| concepts[1].level | 1 |
| concepts[1].score | 0.4882555305957794 |
| concepts[1].wikidata | https://www.wikidata.org/wiki/Q7193 |
| concepts[1].display_name | Microbiology |
| concepts[2].id | https://openalex.org/C86803240 |
| concepts[2].level | 0 |
| concepts[2].score | 0.38103896379470825 |
| concepts[2].wikidata | https://www.wikidata.org/wiki/Q420 |
| concepts[2].display_name | Biology |
| concepts[3].id | https://openalex.org/C501593827 |
| concepts[3].level | 2 |
| concepts[3].score | 0.12224489450454712 |
| concepts[3].wikidata | https://www.wikidata.org/wiki/Q12187 |
| concepts[3].display_name | Antibiotics |
| keywords[0].id | https://openalex.org/keywords/haemophilus-influenzae |
| keywords[0].score | 0.9404406547546387 |
| keywords[0].display_name | Haemophilus influenzae |
| keywords[1].id | https://openalex.org/keywords/microbiology |
| keywords[1].score | 0.4882555305957794 |
| keywords[1].display_name | Microbiology |
| keywords[2].id | https://openalex.org/keywords/biology |
| keywords[2].score | 0.38103896379470825 |
| keywords[2].display_name | Biology |
| keywords[3].id | https://openalex.org/keywords/antibiotics |
| keywords[3].score | 0.12224489450454712 |
| keywords[3].display_name | Antibiotics |
| language | en |
| locations[0].id | doi:10.3389/fcimb.2021.721547 |
| locations[0].is_oa | True |
| locations[0].source.id | https://openalex.org/S2506667490 |
| locations[0].source.issn | 2235-2988 |
| locations[0].source.type | journal |
| locations[0].source.is_oa | True |
| locations[0].source.issn_l | 2235-2988 |
| locations[0].source.is_core | True |
| locations[0].source.is_in_doaj | True |
| locations[0].source.display_name | Frontiers in Cellular and Infection Microbiology |
| locations[0].source.host_organization | https://openalex.org/P4310320527 |
| locations[0].source.host_organization_name | Frontiers Media |
| locations[0].source.host_organization_lineage | https://openalex.org/P4310320527 |
| locations[0].source.host_organization_lineage_names | Frontiers Media |
| locations[0].license | cc-by |
| locations[0].pdf_url | |
| locations[0].version | publishedVersion |
| locations[0].raw_type | journal-article |
| locations[0].license_id | https://openalex.org/licenses/cc-by |
| locations[0].is_accepted | True |
| locations[0].is_published | True |
| locations[0].raw_source_name | Frontiers in Cellular and Infection Microbiology |
| locations[0].landing_page_url | https://doi.org/10.3389/fcimb.2021.721547 |
| locations[1].id | pmid:34631602 |
| locations[1].is_oa | False |
| locations[1].source.id | https://openalex.org/S4306525036 |
| locations[1].source.issn | |
| locations[1].source.type | repository |
| locations[1].source.is_oa | False |
| locations[1].source.issn_l | |
| locations[1].source.is_core | False |
| locations[1].source.is_in_doaj | False |
| locations[1].source.display_name | PubMed |
| locations[1].source.host_organization | https://openalex.org/I1299303238 |
| locations[1].source.host_organization_name | National Institutes of Health |
| locations[1].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[1].license | |
| locations[1].pdf_url | |
| locations[1].version | publishedVersion |
| locations[1].raw_type | |
| locations[1].license_id | |
| locations[1].is_accepted | True |
| locations[1].is_published | True |
| locations[1].raw_source_name | Frontiers in cellular and infection microbiology |
| locations[1].landing_page_url | https://pubmed.ncbi.nlm.nih.gov/34631602 |
| locations[2].id | pmh:oai:doaj.org/article:2a8b061880eb4ab2b541dcaffa28c3cd |
| locations[2].is_oa | True |
| locations[2].source.id | https://openalex.org/S4306401280 |
| locations[2].source.issn | |
| locations[2].source.type | repository |
| locations[2].source.is_oa | False |
| locations[2].source.issn_l | |
| locations[2].source.is_core | False |
| locations[2].source.is_in_doaj | False |
| locations[2].source.display_name | DOAJ (DOAJ: Directory of Open Access Journals) |
| locations[2].source.host_organization | |
| locations[2].source.host_organization_name | |
| locations[2].license | cc-by-sa |
| locations[2].pdf_url | |
| locations[2].version | submittedVersion |
| locations[2].raw_type | article |
| locations[2].license_id | https://openalex.org/licenses/cc-by-sa |
| locations[2].is_accepted | False |
| locations[2].is_published | False |
| locations[2].raw_source_name | Frontiers in Cellular and Infection Microbiology, Vol 11 (2021) |
| locations[2].landing_page_url | https://doaj.org/article/2a8b061880eb4ab2b541dcaffa28c3cd |
| locations[3].id | pmh:oai:europepmc.org:7386422 |
| locations[3].is_oa | True |
| locations[3].source.id | https://openalex.org/S4306400806 |
| locations[3].source.issn | |
| locations[3].source.type | repository |
| locations[3].source.is_oa | False |
| locations[3].source.issn_l | |
| locations[3].source.is_core | False |
| locations[3].source.is_in_doaj | False |
| locations[3].source.display_name | Europe PMC (PubMed Central) |
| locations[3].source.host_organization | https://openalex.org/I1303153112 |
| locations[3].source.host_organization_name | European Bioinformatics Institute |
| locations[3].source.host_organization_lineage | https://openalex.org/I1303153112 |
| locations[3].license | other-oa |
| locations[3].pdf_url | |
| locations[3].version | submittedVersion |
| locations[3].raw_type | Text |
| locations[3].license_id | https://openalex.org/licenses/other-oa |
| locations[3].is_accepted | False |
| locations[3].is_published | False |
| locations[3].raw_source_name | |
| locations[3].landing_page_url | http://europepmc.org/pmc/articles/PMC8493954 |
| locations[4].id | pmh:oai:pubmedcentral.nih.gov:8493954 |
| locations[4].is_oa | True |
| locations[4].source.id | https://openalex.org/S2764455111 |
| locations[4].source.issn | |
| locations[4].source.type | repository |
| locations[4].source.is_oa | False |
| locations[4].source.issn_l | |
| locations[4].source.is_core | False |
| locations[4].source.is_in_doaj | False |
| locations[4].source.display_name | PubMed Central |
| locations[4].source.host_organization | https://openalex.org/I1299303238 |
| locations[4].source.host_organization_name | National Institutes of Health |
| locations[4].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[4].license | other-oa |
| locations[4].pdf_url | |
| locations[4].version | submittedVersion |
| locations[4].raw_type | Text |
| locations[4].license_id | https://openalex.org/licenses/other-oa |
| locations[4].is_accepted | False |
| locations[4].is_published | False |
| locations[4].raw_source_name | Front Cell Infect Microbiol |
| locations[4].landing_page_url | https://www.ncbi.nlm.nih.gov/pmc/articles/8493954 |
| indexed_in | crossref, doaj, pubmed |
| authorships[0].author.id | https://openalex.org/A5068568296 |
| authorships[0].author.orcid | https://orcid.org/0000-0001-6280-4053 |
| authorships[0].author.display_name | Qilong Cao |
| authorships[0].countries | CN |
| authorships[0].affiliations[0].institution_ids | https://openalex.org/I87445476 |
| authorships[0].affiliations[0].raw_affiliation_string | Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, China |
| authorships[0].affiliations[1].institution_ids | https://openalex.org/I4210135740 |
| authorships[0].affiliations[1].raw_affiliation_string | Research and Development Department, Qingdao Haier Biotech Co. Ltd, Qingdao, China |
| authorships[0].institutions[0].id | https://openalex.org/I4210135740 |
| authorships[0].institutions[0].ror | https://ror.org/04a2kft13 |
| authorships[0].institutions[0].type | company |
| authorships[0].institutions[0].lineage | https://openalex.org/I4210135740 |
| authorships[0].institutions[0].country_code | CN |
| authorships[0].institutions[0].display_name | Haier Group (China) |
| authorships[0].institutions[1].id | https://openalex.org/I87445476 |
| authorships[0].institutions[1].ror | https://ror.org/017zhmm22 |
| authorships[0].institutions[1].type | education |
| authorships[0].institutions[1].lineage | https://openalex.org/I87445476 |
| authorships[0].institutions[1].country_code | CN |
| authorships[0].institutions[1].display_name | Xi'an Jiaotong University |
| authorships[0].author_position | first |
| authorships[0].raw_author_name | Qilong Cao |
| authorships[0].is_corresponding | False |
| authorships[0].raw_affiliation_strings | Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, China, Research and Development Department, Qingdao Haier Biotech Co. Ltd, Qingdao, China |
| authorships[1].author.id | https://openalex.org/A5052200721 |
| authorships[1].author.orcid | |
| authorships[1].author.display_name | Shaoshuai Liang |
| authorships[1].countries | CN |
| authorships[1].affiliations[0].institution_ids | https://openalex.org/I4210135740 |
| authorships[1].affiliations[0].raw_affiliation_string | Research and Development Department, Qingdao Haier Biotech Co. Ltd, Qingdao, China |
| authorships[1].institutions[0].id | https://openalex.org/I4210135740 |
| authorships[1].institutions[0].ror | https://ror.org/04a2kft13 |
| authorships[1].institutions[0].type | company |
| authorships[1].institutions[0].lineage | https://openalex.org/I4210135740 |
| authorships[1].institutions[0].country_code | CN |
| authorships[1].institutions[0].display_name | Haier Group (China) |
| authorships[1].author_position | middle |
| authorships[1].raw_author_name | Shaoshuai Liang |
| authorships[1].is_corresponding | False |
| authorships[1].raw_affiliation_strings | Research and Development Department, Qingdao Haier Biotech Co. Ltd, Qingdao, China |
| authorships[2].author.id | https://openalex.org/A5115595526 |
| authorships[2].author.orcid | https://orcid.org/0000-0002-6710-2971 |
| authorships[2].author.display_name | Lin Wang |
| authorships[2].countries | CN |
| authorships[2].affiliations[0].institution_ids | https://openalex.org/I4210135740 |
| authorships[2].affiliations[0].raw_affiliation_string | Research and Development Department, Qingdao Haier Biotech Co. Ltd, Qingdao, China |
| authorships[2].institutions[0].id | https://openalex.org/I4210135740 |
| authorships[2].institutions[0].ror | https://ror.org/04a2kft13 |
| authorships[2].institutions[0].type | company |
| authorships[2].institutions[0].lineage | https://openalex.org/I4210135740 |
| authorships[2].institutions[0].country_code | CN |
| authorships[2].institutions[0].display_name | Haier Group (China) |
| authorships[2].author_position | middle |
| authorships[2].raw_author_name | Lin Wang |
| authorships[2].is_corresponding | False |
| authorships[2].raw_affiliation_strings | Research and Development Department, Qingdao Haier Biotech Co. Ltd, Qingdao, China |
| authorships[3].author.id | https://openalex.org/A5074519834 |
| authorships[3].author.orcid | https://orcid.org/0000-0002-5591-2299 |
| authorships[3].author.display_name | Jun Cao |
| authorships[3].countries | CN |
| authorships[3].affiliations[0].institution_ids | https://openalex.org/I126520041 |
| authorships[3].affiliations[0].raw_affiliation_string | Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China |
| authorships[3].institutions[0].id | https://openalex.org/I126520041 |
| authorships[3].institutions[0].ror | https://ror.org/04c4dkn09 |
| authorships[3].institutions[0].type | education |
| authorships[3].institutions[0].lineage | https://openalex.org/I126520041, https://openalex.org/I19820366 |
| authorships[3].institutions[0].country_code | CN |
| authorships[3].institutions[0].display_name | University of Science and Technology of China |
| authorships[3].author_position | middle |
| authorships[3].raw_author_name | Jun Cao |
| authorships[3].is_corresponding | False |
| authorships[3].raw_affiliation_strings | Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China |
| authorships[4].author.id | https://openalex.org/A5100462410 |
| authorships[4].author.orcid | https://orcid.org/0000-0002-1985-3500 |
| authorships[4].author.display_name | Mengyang Liu |
| authorships[4].countries | CN |
| authorships[4].affiliations[0].institution_ids | https://openalex.org/I108688024, https://openalex.org/I4210116869 |
| authorships[4].affiliations[0].raw_affiliation_string | Department of Clinical Laboratory, The Affiliated Hospital of Qingdao University, Qingdao, China |
| authorships[4].institutions[0].id | https://openalex.org/I4210116869 |
| authorships[4].institutions[0].ror | https://ror.org/026e9yy16 |
| authorships[4].institutions[0].type | healthcare |
| authorships[4].institutions[0].lineage | https://openalex.org/I4210116869 |
| authorships[4].institutions[0].country_code | CN |
| authorships[4].institutions[0].display_name | Affiliated Hospital of Qingdao University |
| authorships[4].institutions[1].id | https://openalex.org/I108688024 |
| authorships[4].institutions[1].ror | https://ror.org/021cj6z65 |
| authorships[4].institutions[1].type | education |
| authorships[4].institutions[1].lineage | https://openalex.org/I108688024 |
| authorships[4].institutions[1].country_code | CN |
| authorships[4].institutions[1].display_name | Qingdao University |
| authorships[4].author_position | middle |
| authorships[4].raw_author_name | Mengyang Liu |
| authorships[4].is_corresponding | False |
| authorships[4].raw_affiliation_strings | Department of Clinical Laboratory, The Affiliated Hospital of Qingdao University, Qingdao, China |
| authorships[5].author.id | https://openalex.org/A5009676248 |
| authorships[5].author.orcid | https://orcid.org/0009-0004-0989-9793 |
| authorships[5].author.display_name | Shengpeng Li |
| authorships[5].affiliations[0].raw_affiliation_string | Department of Clinical Laboratory, Qingdao Youfu Hospital, Qingdao, China |
| authorships[5].author_position | middle |
| authorships[5].raw_author_name | Shengpeng Li |
| authorships[5].is_corresponding | False |
| authorships[5].raw_affiliation_strings | Department of Clinical Laboratory, Qingdao Youfu Hospital, Qingdao, China |
| authorships[6].author.id | https://openalex.org/A5016059940 |
| authorships[6].author.orcid | https://orcid.org/0000-0001-8124-7491 |
| authorships[6].author.display_name | Xiaolong Cao |
| authorships[6].countries | CN |
| authorships[6].affiliations[0].institution_ids | https://openalex.org/I4210107745 |
| authorships[6].affiliations[0].raw_affiliation_string | Outpatient Department, Beijing Changping Institute for Tuberculosis Prevention and Treatment, Beijing, China |
| authorships[6].institutions[0].id | https://openalex.org/I4210107745 |
| authorships[6].institutions[0].ror | https://ror.org/01p2t4k13 |
| authorships[6].institutions[0].type | healthcare |
| authorships[6].institutions[0].lineage | https://openalex.org/I4210107745 |
| authorships[6].institutions[0].country_code | CN |
| authorships[6].institutions[0].display_name | Heilongjiang Center for Tuberculosis Control and Prevention |
| authorships[6].author_position | middle |
| authorships[6].raw_author_name | Xiaolong Cao |
| authorships[6].is_corresponding | True |
| authorships[6].raw_affiliation_strings | Outpatient Department, Beijing Changping Institute for Tuberculosis Prevention and Treatment, Beijing, China |
| authorships[7].author.id | https://openalex.org/A5059564497 |
| authorships[7].author.orcid | https://orcid.org/0000-0002-7364-2392 |
| authorships[7].author.display_name | Yan Guo |
| authorships[7].countries | CN |
| authorships[7].affiliations[0].institution_ids | https://openalex.org/I87445476 |
| authorships[7].affiliations[0].raw_affiliation_string | Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, China |
| authorships[7].institutions[0].id | https://openalex.org/I87445476 |
| authorships[7].institutions[0].ror | https://ror.org/017zhmm22 |
| authorships[7].institutions[0].type | education |
| authorships[7].institutions[0].lineage | https://openalex.org/I87445476 |
| authorships[7].institutions[0].country_code | CN |
| authorships[7].institutions[0].display_name | Xi'an Jiaotong University |
| authorships[7].author_position | last |
| authorships[7].raw_author_name | Yan Guo |
| authorships[7].is_corresponding | True |
| authorships[7].raw_affiliation_strings | Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, China |
| has_content.pdf | False |
| has_content.grobid_xml | False |
| is_paratext | False |
| open_access.is_oa | True |
| open_access.oa_url | https://doi.org/10.3389/fcimb.2021.721547 |
| open_access.oa_status | gold |
| open_access.any_repository_has_fulltext | False |
| created_date | 2025-10-10T00:00:00 |
| display_name | A Rapid Detection of Haemophilus influenzae Using Multiple Cross Displacement Amplification Linked With Nanoparticle-Based Lateral Flow Biosensor |
| has_fulltext | False |
| is_retracted | False |
| updated_date | 2025-11-06T03:46:38.306776 |
| primary_topic.id | https://openalex.org/T10873 |
| primary_topic.field.id | https://openalex.org/fields/24 |
| primary_topic.field.display_name | Immunology and Microbiology |
| primary_topic.score | 0.9998000264167786 |
| primary_topic.domain.id | https://openalex.org/domains/1 |
| primary_topic.domain.display_name | Life Sciences |
| primary_topic.subfield.id | https://openalex.org/subfields/2404 |
| primary_topic.subfield.display_name | Microbiology |
| primary_topic.display_name | Bacterial Infections and Vaccines |
| related_works | https://openalex.org/W1979424193, https://openalex.org/W2105874304, https://openalex.org/W2017287049, https://openalex.org/W2386345146, https://openalex.org/W2591120629, https://openalex.org/W2114205969, https://openalex.org/W2033986591, https://openalex.org/W2391939020, https://openalex.org/W2488825137, https://openalex.org/W1967966451 |
| cited_by_count | 9 |
| counts_by_year[0].year | 2025 |
| counts_by_year[0].cited_by_count | 1 |
| counts_by_year[1].year | 2024 |
| counts_by_year[1].cited_by_count | 5 |
| counts_by_year[2].year | 2022 |
| counts_by_year[2].cited_by_count | 2 |
| counts_by_year[3].year | 2021 |
| counts_by_year[3].cited_by_count | 1 |
| locations_count | 5 |
| best_oa_location.id | doi:10.3389/fcimb.2021.721547 |
| best_oa_location.is_oa | True |
| best_oa_location.source.id | https://openalex.org/S2506667490 |
| best_oa_location.source.issn | 2235-2988 |
| best_oa_location.source.type | journal |
| best_oa_location.source.is_oa | True |
| best_oa_location.source.issn_l | 2235-2988 |
| best_oa_location.source.is_core | True |
| best_oa_location.source.is_in_doaj | True |
| best_oa_location.source.display_name | Frontiers in Cellular and Infection Microbiology |
| best_oa_location.source.host_organization | https://openalex.org/P4310320527 |
| best_oa_location.source.host_organization_name | Frontiers Media |
| best_oa_location.source.host_organization_lineage | https://openalex.org/P4310320527 |
| best_oa_location.source.host_organization_lineage_names | Frontiers Media |
| best_oa_location.license | cc-by |
| best_oa_location.pdf_url | |
| best_oa_location.version | publishedVersion |
| best_oa_location.raw_type | journal-article |
| best_oa_location.license_id | https://openalex.org/licenses/cc-by |
| best_oa_location.is_accepted | True |
| best_oa_location.is_published | True |
| best_oa_location.raw_source_name | Frontiers in Cellular and Infection Microbiology |
| best_oa_location.landing_page_url | https://doi.org/10.3389/fcimb.2021.721547 |
| primary_location.id | doi:10.3389/fcimb.2021.721547 |
| primary_location.is_oa | True |
| primary_location.source.id | https://openalex.org/S2506667490 |
| primary_location.source.issn | 2235-2988 |
| primary_location.source.type | journal |
| primary_location.source.is_oa | True |
| primary_location.source.issn_l | 2235-2988 |
| primary_location.source.is_core | True |
| primary_location.source.is_in_doaj | True |
| primary_location.source.display_name | Frontiers in Cellular and Infection Microbiology |
| primary_location.source.host_organization | https://openalex.org/P4310320527 |
| primary_location.source.host_organization_name | Frontiers Media |
| primary_location.source.host_organization_lineage | https://openalex.org/P4310320527 |
| primary_location.source.host_organization_lineage_names | Frontiers Media |
| primary_location.license | cc-by |
| primary_location.pdf_url | |
| primary_location.version | publishedVersion |
| primary_location.raw_type | journal-article |
| primary_location.license_id | https://openalex.org/licenses/cc-by |
| primary_location.is_accepted | True |
| primary_location.is_published | True |
| primary_location.raw_source_name | Frontiers in Cellular and Infection Microbiology |
| primary_location.landing_page_url | https://doi.org/10.3389/fcimb.2021.721547 |
| publication_date | 2021-09-22 |
| publication_year | 2021 |
| referenced_works | https://openalex.org/W2145022921, https://openalex.org/W2076746345, https://openalex.org/W2120399147, https://openalex.org/W1787157507, https://openalex.org/W2082700337, https://openalex.org/W2112324283, https://openalex.org/W2120162013, https://openalex.org/W2006825138, https://openalex.org/W1976099212, https://openalex.org/W1985168330, https://openalex.org/W2091575535, https://openalex.org/W3080346424, https://openalex.org/W2105975903, https://openalex.org/W1782814620, https://openalex.org/W2323185543, https://openalex.org/W1864971727, https://openalex.org/W1926738805, https://openalex.org/W1955467646, https://openalex.org/W2150219559, https://openalex.org/W4391337513, https://openalex.org/W2125943365, https://openalex.org/W2089399380, https://openalex.org/W2561224773, https://openalex.org/W269088179, https://openalex.org/W2767209003, https://openalex.org/W2202465248, https://openalex.org/W2022800117, https://openalex.org/W2162917963, https://openalex.org/W2165827376 |
| referenced_works_count | 29 |
| abstract_inverted_index., | 52, 220 |
| abstract_inverted_index.. | 26, 50, 69, 94, 117, 124, 137, 218, 232 |
| abstract_inverted_index.1 | 88 |
| abstract_inverted_index.H | 25, 49, 68, 93, 116, 123, 136, 217, 231 |
| abstract_inverted_index.a | 3, 10, 226 |
| abstract_inverted_index.10 | 76 |
| abstract_inverted_index.21 | 154 |
| abstract_inverted_index.56 | 145 |
| abstract_inverted_index.In | 29, 144, 202 |
| abstract_inverted_index.On | 64 |
| abstract_inverted_index.P6 | 74 |
| abstract_inverted_index.an | 192 |
| abstract_inverted_index.as | 15, 225 |
| abstract_inverted_index.be | 58, 223 |
| abstract_inverted_index.h. | 89 |
| abstract_inverted_index.in | 9, 99, 134, 160, 173, 234 |
| abstract_inverted_index.is | 2, 21, 207 |
| abstract_inverted_index.it | 20 |
| abstract_inverted_index.no | 130 |
| abstract_inverted_index.of | 12, 67, 92, 106, 115, 169, 171, 216 |
| abstract_inverted_index.to | 23, 47, 57 |
| abstract_inverted_index.100 | 111 |
| abstract_inverted_index.DNA | 108 |
| abstract_inverted_index.PCR | 152, 181 |
| abstract_inverted_index.The | 81, 90, 113, 167 |
| abstract_inverted_index.and | 38, 61, 129, 140, 151, 180, 213, 236 |
| abstract_inverted_index.can | 183 |
| abstract_inverted_index.fg. | 112 |
| abstract_inverted_index.for | 87, 198, 209, 230 |
| abstract_inverted_index.has | 54 |
| abstract_inverted_index.not | 62 |
| abstract_inverted_index.the | 32, 65, 100, 103, 163, 176, 188, 195, 204, 210 |
| abstract_inverted_index.was | 85, 110, 132, 159 |
| abstract_inverted_index.MCDA | 83 |
| abstract_inverted_index.been | 55 |
| abstract_inverted_index.best | 82 |
| abstract_inverted_index.flow | 41 |
| abstract_inverted_index.hard | 22 |
| abstract_inverted_index.non- | 135 |
| abstract_inverted_index.only | 122 |
| abstract_inverted_index.over | 194 |
| abstract_inverted_index.pure | 101 |
| abstract_inverted_index.such | 14 |
| abstract_inverted_index.that | 187 |
| abstract_inverted_index.this | 30 |
| abstract_inverted_index.tool | 229 |
| abstract_inverted_index.used | 224 |
| abstract_inverted_index.were | 45, 79, 127, 156 |
| abstract_inverted_index.with | 148, 162, 175 |
| abstract_inverted_index.(LFB) | 43 |
| abstract_inverted_index.100%, | 185 |
| abstract_inverted_index.61°C | 86 |
| abstract_inverted_index.assay | 97, 120, 190, 206 |
| abstract_inverted_index.basic | 235 |
| abstract_inverted_index.basis | 66 |
| abstract_inverted_index.cross | 34 |
| abstract_inverted_index.found | 133 |
| abstract_inverted_index.gains | 191 |
| abstract_inverted_index.gene, | 75 |
| abstract_inverted_index.human | 5 |
| abstract_inverted_index.major | 4 |
| abstract_inverted_index.might | 222 |
| abstract_inverted_index.other | 141 |
| abstract_inverted_index.outer | 71 |
| abstract_inverted_index.reach | 184 |
| abstract_inverted_index.which | 53, 158, 221 |
| abstract_inverted_index.(MCDA) | 37 |
| abstract_inverted_index.detect | 48 |
| abstract_inverted_index.method | 150, 179, 197 |
| abstract_inverted_index.proven | 56 |
| abstract_inverted_index.rapid, | 60, 212 |
| abstract_inverted_index.series | 11 |
| abstract_inverted_index.showed | 121 |
| abstract_inverted_index.sputum | 146 |
| abstract_inverted_index.study, | 31 |
| abstract_inverted_index.target | 199 |
| abstract_inverted_index.culture | 165, 178 |
| abstract_inverted_index.genomic | 107 |
| abstract_inverted_index.lateral | 40 |
| abstract_inverted_index.method. | 166 |
| abstract_inverted_index.minimum | 104 |
| abstract_inverted_index.primers | 78 |
| abstract_inverted_index.protein | 73 |
| abstract_inverted_index.samples | 155 |
| abstract_inverted_index.showed, | 98 |
| abstract_inverted_index.-MCD-LFB | 96, 119 |
| abstract_inverted_index.However, | 19 |
| abstract_inverted_index.MCDA-LFB | 149, 189, 205 |
| abstract_inverted_index.accuracy | 168 |
| abstract_inverted_index.clinical | 237 |
| abstract_inverted_index.combined | 46 |
| abstract_inverted_index.diagnose | 24 |
| abstract_inverted_index.isolates | 139 |
| abstract_inverted_index.membrane | 72 |
| abstract_inverted_index.multiple | 33 |
| abstract_inverted_index.pathogen | 200 |
| abstract_inverted_index.quickly. | 28 |
| abstract_inverted_index.samples, | 147 |
| abstract_inverted_index.species. | 143 |
| abstract_inverted_index.specific | 77, 214 |
| abstract_inverted_index.suitable | 208 |
| abstract_inverted_index.MCDA-LFB, | 172 |
| abstract_inverted_index.advantage | 193 |
| abstract_inverted_index.biosensor | 42 |
| abstract_inverted_index.condition | 84 |
| abstract_inverted_index.cultures, | 102 |
| abstract_inverted_index.designed. | 80 |
| abstract_inverted_index.detected, | 128 |
| abstract_inverted_index.detection | 215 |
| abstract_inverted_index.diagnosis | 170 |
| abstract_inverted_index.diseases, | 13 |
| abstract_inverted_index.positive, | 157 |
| abstract_inverted_index.potential | 227 |
| abstract_inverted_index.reliable, | 59 |
| abstract_inverted_index.resulting | 8 |
| abstract_inverted_index.templates | 109, 126 |
| abstract_inverted_index.(MCDA-LFB) | 44 |
| abstract_inverted_index.bacterium, | 7 |
| abstract_inverted_index.comparison | 174 |
| abstract_inverted_index.consistent | 161 |
| abstract_inverted_index.detection, | 153, 182 |
| abstract_inverted_index.detection. | 201 |
| abstract_inverted_index.diagnostic | 228 |
| abstract_inverted_index.indicating | 186 |
| abstract_inverted_index.influenzae | 1, 27, 51, 70, 95, 118, 125, 138, 219, 233 |
| abstract_inverted_index.pathogenic | 6 |
| abstract_inverted_index.pneumonia, | 16 |
| abstract_inverted_index.sensitive, | 211 |
| abstract_inverted_index.Haemophilus | 0, 142 |
| abstract_inverted_index.bacteremia, | 17 |
| abstract_inverted_index.conclusion, | 203 |
| abstract_inverted_index.meningitis. | 18 |
| abstract_inverted_index.sensitivity | 91 |
| abstract_inverted_index.specificity | 114 |
| abstract_inverted_index.traditional | 164, 177 |
| abstract_inverted_index.complicated. | 63 |
| abstract_inverted_index.displacement | 35 |
| abstract_inverted_index.amplification | 36 |
| abstract_inverted_index.concentration | 105 |
| abstract_inverted_index.laboratories. | 238 |
| abstract_inverted_index.cultured-based | 196 |
| abstract_inverted_index.cross-reactivity | 131 |
| abstract_inverted_index.nanoparticle-based | 39 |
| cited_by_percentile_year.max | 98 |
| cited_by_percentile_year.min | 89 |
| corresponding_author_ids | https://openalex.org/A5059564497, https://openalex.org/A5016059940 |
| countries_distinct_count | 1 |
| institutions_distinct_count | 8 |
| corresponding_institution_ids | https://openalex.org/I4210107745, https://openalex.org/I87445476 |
| citation_normalized_percentile.value | 0.7750503 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |