A Simple Multistep Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional Macrophages Article Swipe

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Chandrayana Mukherjee , Christine Hale , Subhankar Mukhopadhyay ·

YOU? · · 2018 · Open Access · · DOI: https://doi.org/10.1007/978-1-4939-7837-3_2

Macrophages differentiated from human induced pluripotent stem cells (hiPSCs) provide an alternative new tool overcoming some of the limitations of existing models for human macrophages, such as human macrophage-like cell lines and primary monocyte-derived macrophages. A combination of different cytokines and growth factors can differentiate hiPSCs toward myeloid lineage. Here we describe a simple multistep protocol for differentiating hiPSCs into functional macrophages. This includes derivation of three germ-line containing embryoid bodies (EBs) from iPSCs, generation of myeloid precursors from EBs, and finally maturation of myeloid precursors into functional macrophages. Technical procedure and specific culture conditions associated with each of these steps are discussed in detail.

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Embryoid body Induced pluripotent stem cell Human Induced Pluripotent Stem Cells Myeloid Cell biology Myeloid cells Macrophage Biology Cell culture Chemistry Immunology Embryonic stem cell Genetics In vitro Gene

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Type: article
Language: en
Landing Page: https://doi.org/10.1007/978-1-4939-7837-3_2
PDF: https://link.springer.com/content/pdf/10.1007%2F978-1-4939-7837-3_2.pdf
OA Status: hybrid
Cited By: 33
References: 23
Related Works: 10
OpenAlex ID: https://openalex.org/W2803429166

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OpenAlex ID: https://openalex.org/W2803429166

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DOI: https://doi.org/10.1007/978-1-4939-7837-3_2

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Title: A Simple Multistep Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional Macrophages

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Type: article

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Language: en

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Publication year: 2018

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Publication date: 2018-01-01

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Authors: Chandrayana Mukherjee, Christine Hale, Subhankar Mukhopadhyay

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Landing page: https://doi.org/10.1007/978-1-4939-7837-3_2

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PDF URL: https://link.springer.com/content/pdf/10.1007%2F978-1-4939-7837-3_2.pdf

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Concepts: Embryoid body, Induced pluripotent stem cell, Human Induced Pluripotent Stem Cells, Myeloid, Cell biology, Myeloid cells, Macrophage, Biology, Cell culture, Chemistry, Immunology, Embryonic stem cell, Genetics, In vitro, Gene

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Cited by: 33

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Citations by year (recent): 2025: 5, 2024: 6, 2023: 4, 2022: 8, 2021: 4

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