A simplified and easy‐to‐use HIP HOP assay provides insights into chalcone antifungal mechanisms of action
Article Swipe
Thomas A. K. Prescott
,
Laura Anaissi‐Afonso
,
Keith R. Fox
,
Anthony Maxwell
,
Barry Panaretou
,
Félix Machín
·
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.1002/1873-3468.14483
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.1002/1873-3468.14483
Elucidating the mechanism of action of an antifungal or cytotoxic compound is a time‐consuming process. Yeast chemogenomic profiling provides a compelling solution to the problem but is experimentally complex. Here, we demonstrate the use of a highly simplified yeast chemical genetic assay comprising just 89 yeast deletion strains, each diagnostic for a specific mechanism of action. We use the assay to investigate the mechanism of action of two antifungal chalcone compounds, trans ‐chalcone and 4′‐hydroxychalcone, and narrow down the mechanism to transcriptional stress. Crucially, the assay eliminates mechanisms of action such as topoisomerase I inhibition and membrane disruption that have been suggested for related chalcone compounds. We propose this simplified assay as a useful tool to rapidly identify common off‐target mechanisms.
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1002/1873-3468.14483
- OA Status
- hybrid
- Cited By
- 1
- References
- 30
- Related Works
- 10
- OpenAlex ID
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All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4293093281Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1002/1873-3468.14483Digital Object Identifier
- Title
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A simplified and easy‐to‐use
HIP HOP assay provides insights into chalcone antifungal mechanisms of actionWork title - Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2022Year of publication
- Publication date
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2022-08-26Full publication date if available
- Authors
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Thomas A. K. Prescott, Laura Anaissi‐Afonso, Keith R. Fox, Anthony Maxwell, Barry Panaretou, Félix MachínList of authors in order
- Landing page
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https://doi.org/10.1002/1873-3468.14483Publisher landing page
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YesWhether a free full text is available
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hybridOpen access status per OpenAlex
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https://doi.org/10.1002/1873-3468.14483Direct OA link when available
- Concepts
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Chalcone, Mechanism of action, Antifungal, Saccharomyces cerevisiae, Yeast, Chemistry, Computational biology, Biochemistry, Biology, Chalcone synthase, Gene, Stereochemistry, Microbiology, Gene expression, In vitroTop concepts (fields/topics) attached by OpenAlex
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1Total citation count in OpenAlex
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2024: 1Per-year citation counts (last 5 years)
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10Other works algorithmically related by OpenAlex
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