A single-cell atlas of immune checkpoint molecules in atherosclerosis in ApoE-/- mice Article Swipe

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Venetia Bazioti , Laura A. Bosmans , Claudia M. van Tiel , Markus Joppich , David Ahern , Annelie Shami , Christian Weber , Menno M P J de Winther , Dorothee Atzler , Claudia Monaco , Esther Lutgens ·

YOU? · · 2023 · Open Access · · DOI: https://doi.org/10.1093/eurheartj/ehad655.3296

Introduction Immune checkpoint (IC) targeting is an attractive way to combat atherosclerosis, since ICs regulate the immune response by activating or inhibiting inflammatory pathways. We have shown that ICs exert cell-divergent functions and that cell-specific IC-based treatments can limit immune-related side effects. To design tailored IC-based immunotherapies for atherosclerosis, a detailed single-cell atlas of IC expression during atherogenesis is required. Purpose To obtain cell-type- and atherosclerosis-stage-specific expression profiles of ICs and assess the transcriptome of IC+ cells during atherosclerosis. Methods ApoE-/- mice were fed normal chow diet (NCD) for 10, 20 or 30 weeks, or high-cholesterol diet (HCD) for 6, 10, 14 (study 1) or 20 weeks (study 2). Using mass-cytometry by time of flight (CyTOF), we assessed the cell-type-specific aortic expression of ICs during atheroprogression (study 1). Using cellular indexing of transcriptomes and epitopes by sequencing (CITE-Seq), we assessed the transcriptome of aortic IC+ and IC- cells (study 2). Results Using CyTOF, we identified 21 immune cell clusters, including T and B cells, Ly6C- and Ly6C+ monocytes and macrophage (mφ) subsets. On T cell subsets, IC expression remained relatively constant in early plaques independent of the type of diet, apart from an increase in CD40-CD40L and decrease in CD28 and PD-L1 in HCD-fed mice. In advanced plaques from NCD-fed mice, most T cell subsets showed increased PD-L1, GITRL and CD27, while in HCD-fed mice, CD40L was decreased and PD-L1, PD-L2, CD27, CD86-CD28 and GITR-GITRL were induced on these T cell subsets. On myeloid cells, IC expression changed during early atherogenesis. Specifically, in NCD-fed mice, CD11c+MHCII+ mφ upregulated CD40 and PD-L1 and downregulated CD27 and PD-L2, while dendritic cells increased GITR and decreased PD-L2. Additional ICs, such as GITRL, CD40L and CD86-CD28, were induced in advanced plaques from NCD-fed mice. In HCD-fed mice, numerous ICs, i.e. CD40-CD40L, GITR-GITRL, CD86-CD28 and PD-L2, were increased in myeloid subsets in both early and advanced plaques. Using CITE-Seq, we further studied ICs that were strongly regulated during atherogenesis, e.g. GITR. Gene Ontology enrichment analysis of GITR+ and GITR- cells within the myeloid and T cell subsets identified the regulation of various pathways: When compared to their GITR- counterparts, both CD25- and CD25+ CD4+GITR+ T cell subsets showed upregulated pathways of death receptor activity, while CD25+ cells also showed induction of pathways involved in transmembrane lipid transporter activity. In mφ, GITR+ mφ showed upregulated pathways of cell adhesion and cytokine activity compared to GITR- mφ. Conclusion We have identified the expression pattern of numerous ICs on aortic immune cell subsets at various atherosclerosis stages. These data will pave the way towards the development of selective IC-based therapies to combat atherosclerosis.

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CD86 Immune system Medicine T cell CD40 Immune checkpoint Transcriptome CD28 Cancer research Cell Immunology Immunotherapy Cytotoxic T cell Biology Gene expression Biochemistry In vitro Gene

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Language: en
Landing Page: https://doi.org/10.1093/eurheartj/ehad655.3296
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Title: A single-cell atlas of immune checkpoint molecules in atherosclerosis in ApoE-/- mice

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Publication year: 2023

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Publication date: 2023-11-01

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Authors: Venetia Bazioti, Laura A. Bosmans, Claudia M. van Tiel, Markus Joppich, David Ahern, Annelie Shami, Christian Weber, Menno M P J de Winther, Dorothee Atzler, Claudia Monaco, Esther Lutgens

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Concepts: CD86, Immune system, Medicine, T cell, CD40, Immune checkpoint, Transcriptome, CD28, Cancer research, Cell, Immunology, Immunotherapy, Cytotoxic T cell, Biology, Gene expression, Biochemistry, In vitro, Gene

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