A single-nucleotide variant conditions the ability vs. inability of Propionibacterium freudenreichii to utilize L-lactate Article Swipe
Riccardo Cocuzzi
,
Meral Turgay
,
Remo S. Schmidt
,
Ueli von Ah
,
H Bachmann
,
Laure Weisskopf
,
Marie‐Therese Fröhlich‐Wyder
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1128/aem.00599-25
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1128/aem.00599-25
Propionibacterium freudenreichii ( P. freudenreichii ) has various biotechnological applications, notably in the ripening of Swiss-type cheese, where it utilizes the two enantiomers of lactate as the main carbon source, contributing to flavor development and eye formation. Here, we genotypically characterized two P. freudenreichii strains unable to catabolize L-lactate through whole-genome sequencing and variant calling, using P. freudenreichii FAM-14222 as the reference genome, which highlighted a mutation in the lutB gene in both strains. This gene is part of the lutABC operon, which has been previously linked to lactate utilization in other bacterial species. Subsequently, we successfully restored the strains’ ability to utilize L-lactate by following an adaptive laboratory evolution approach, which involved repeated subculturing in a medium containing L-lactate as the main carbon source. Sequencing of the lutB gene confirmed that isolates with a restored ability to utilize L-lactate had also reverted the mutation back to wild-type, supporting the involvement of the lutABC operon in L-lactate catabolism in P. freudenreichii . Moreover, the phenotype of the two L-lactate-negative strains was confirmed under cheesemaking conditions, highlighting the potential of the strains as cheese ripening cultures. IMPORTANCE Lactate catabolism is of paramount importance in Propionibacterium freudenreichii , particularly for its industrial applications, such as Swiss-type cheese ripening. Nevertheless, the genetic background of this metabolic process is not fully understood. In our study, we developed an adaptive laboratory evolution-based approach for the elucidation of L-lactate catabolism, starting from two strains unable to utilize L-lactate. Our results delivered experimental evidence of the role of the lutABC operon in this process, as opposed to the widespread theory of L-lactate dehydrogenase-mediated oxidation. A deeper understanding of this metabolic pathway will be beneficial for a more efficient selection of industrial strains, as well as for metabolic engineering.
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- Type
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- Language
- en
- Landing Page
- https://doi.org/10.1128/aem.00599-25
- OA Status
- diamond
- References
- 30
- Related Works
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- OpenAlex ID
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Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4411240123Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1128/aem.00599-25Digital Object Identifier
- Title
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A single-nucleotide variant conditions the ability vs. inability of Propionibacterium freudenreichii to utilize L-lactateWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2025Year of publication
- Publication date
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2025-06-12Full publication date if available
- Authors
-
Riccardo Cocuzzi, Meral Turgay, Remo S. Schmidt, Ueli von Ah, H Bachmann, Laure Weisskopf, Marie‐Therese Fröhlich‐WyderList of authors in order
- Landing page
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https://doi.org/10.1128/aem.00599-25Publisher landing page
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YesWhether a free full text is available
- OA status
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diamondOpen access status per OpenAlex
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https://doi.org/10.1128/aem.00599-25Direct OA link when available
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Propionibacterium freudenreichii, Propionibacterium, Nucleotide, Biology, Genetics, Bacteria, Chemistry, Food science, Computational biology, GeneTop concepts (fields/topics) attached by OpenAlex
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0Total citation count in OpenAlex
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30Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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