A spatially-tracked single cell transcriptomics map of neuronal networks in the intrinsic cardiac nervous system Article Swipe

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Alison Moss , Shaina Robbins , Sirisha Achanta , Lakshmi Kuttippurathu , Scott Turick , Sean Nieves , Peter Hanna , Elizabeth Smith , Donald B. Hoover , Jin Chen , Zixi Cheng , Jeffrey L. Ardell , Kalyanam Shivkumar , James S. Schwaber , Rajanikanth Vadigepalli ·

YOU? · · 2020 · Open Access · · DOI: https://doi.org/10.1101/2020.07.29.227090

We developed a spatially-tracked single neuron transcriptomics map of an intrinsic cardiac ganglion - the right atrial ganglionic plexus (RAGP) that is a critical mediator of vagal control of the sinoatrial node (SAN) activity. We developed a 3D representation of RAGP with extensive mapping of neurons and used neuronal tracing to identify the spatial distribution of the subset of neurons that project to the SAN. RNAseq of laser capture microdissected neurons revealed a distinct composition of RAGP neurons compared to CNS neuronal subtypes. High-throughput qPCR of hundreds of laser capture microdissected single neurons led to a surprising finding that cholinergic and catecholaminergic neuronal markers Th and Chat were correlated, suggesting multipotential phenotypes that can drive neuroplasticity within RAGP. Interestingly, no single gene or module was an exclusive marker of RAGP neuronal connectivity to SAN. Neuropeptide-receptor coexpression analysis revealed a combinatorial paracrine neuromodulatory network within RAGP, informing follow-on studies on the vagal control of RAGP to regulate cardiac function in health and disease.

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Neuroscience Biology Catecholaminergic Cholinergic Premovement neuronal activity Catecholaminergic cell groups Neuron Sinoatrial node Cholinergic neuron Cellular neuroscience Endocrinology Dopamine Blood pressure Heart rate

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Type: preprint
Language: en
Landing Page: https://doi.org/10.1101/2020.07.29.227090
PDF: https://www.biorxiv.org/content/biorxiv/early/2020/07/30/2020.07.29.227090.full.pdf
OA Status: green
Cited By: 2
References: 60
Related Works: 10
OpenAlex ID: https://openalex.org/W4249061221

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DOI: https://doi.org/10.1101/2020.07.29.227090

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Title: A spatially-tracked single cell transcriptomics map of neuronal networks in the intrinsic cardiac nervous system

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Type: preprint

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Language: en

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Publication year: 2020

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Publication date: 2020-07-30

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Authors: Alison Moss, Shaina Robbins, Sirisha Achanta, Lakshmi Kuttippurathu, Scott Turick, Sean Nieves, Peter Hanna, Elizabeth Smith, Donald B. Hoover, Jin Chen, Zixi Cheng, Jeffrey L. Ardell, Kalyanam Shivkumar, James S. Schwaber, Rajanikanth Vadigepalli

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Landing page: https://doi.org/10.1101/2020.07.29.227090

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PDF URL: https://www.biorxiv.org/content/biorxiv/early/2020/07/30/2020.07.29.227090.full.pdf

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Concepts: Neuroscience, Biology, Catecholaminergic, Cholinergic, Premovement neuronal activity, Catecholaminergic cell groups, Neuron, Sinoatrial node, Cholinergic neuron, Cellular neuroscience, Endocrinology, Dopamine, Blood pressure, Heart rate

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Cited by: 2

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Related works (count): 10

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