A Stable Chemokine Gradient Controls Directional Persistence of Migrating Dendritic Cells Article Swipe
Thomas Quast
,
Karolin Zölzer
,
Donald Guu
,
Luis Álvarez
,
Carsten Küsters
,
Eva Kiermaier
,
U. Benjamin Kaupp
,
Waldemar Kolanus
·
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.3389/fcell.2022.943041
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.3389/fcell.2022.943041
Navigation of dendritic cells (DCs) from the site of infection to lymphoid organs is guided by concentration gradients of CCR7 ligands. How cells interpret chemokine gradients and how they couple directional sensing to polarization and persistent chemotaxis has remained largely elusive. Previous experimental systems were limited in the ability to control fast de novo formation of the final gradient slope, long-lasting stability of the gradient and to expose cells to dynamic stimulation. Here, we used a combination of microfluidics and quantitative in vitro live cell imaging to elucidate the chemotactic sensing strategy of DCs. The microfluidic approach allows us to generate soluble gradients with high spatio-temporal precision and to analyze actin dynamics, cell polarization, and persistent directional migration in both static and dynamic environments. We demonstrate that directional persistence of DC migration requires steady-state characteristics of the soluble gradient instead of temporally rising CCL19 concentration, implying that spatial sensing mechanisms control chemotaxis of DCs. Kymograph analysis of actin dynamics revealed that the presence of the CCL19 gradient is essential to stabilize leading edge protrusions in DCs and to determine directionality, since both cytoskeletal polarization and persistent chemotaxis are abrogated in the range of seconds when steady-state gradients are perturbed. In contrast to Dictyostelium amoeba, DCs are unable to decode oscillatory stimulation of soluble chemokine traveling waves into a directional response toward the wave source. These findings are consistent with the notion that DCs do not employ adaptive temporal sensing strategies that discriminate temporally increasing and decreasing chemoattractant concentrations in our setting. Taken together, in our experimental system DCs do not depend on increasing absolute chemokine concentration over time to induce persistent migration and do not integrate oscillatory stimulation. The observed capability of DCs to migrate with high directional persistence in stable gradients but not when subjected to periodic temporal cues, identifies spatial sensing as a key requirement for persistent chemotaxis of DCs.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3389/fcell.2022.943041
- OA Status
- gold
- Cited By
- 8
- References
- 60
- Related Works
- 10
- OpenAlex ID
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All OpenAlex metadata
Raw OpenAlex JSON
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https://openalex.org/W4292457778Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.3389/fcell.2022.943041Digital Object Identifier
- Title
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A Stable Chemokine Gradient Controls Directional Persistence of Migrating Dendritic CellsWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2022Year of publication
- Publication date
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2022-08-09Full publication date if available
- Authors
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Thomas Quast, Karolin Zölzer, Donald Guu, Luis Álvarez, Carsten Küsters, Eva Kiermaier, U. Benjamin Kaupp, Waldemar KolanusList of authors in order
- Landing page
-
https://doi.org/10.3389/fcell.2022.943041Publisher landing page
- Open access
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YesWhether a free full text is available
- OA status
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goldOpen access status per OpenAlex
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https://doi.org/10.3389/fcell.2022.943041Direct OA link when available
- Concepts
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Chemotaxis, CCL19, Cell migration, Pseudopodia, Traveling wave, Cell polarity, Microfluidics, Biology, Cell biology, Cytoskeleton, Chemokine, Actin, Biophysics, Cell, Chemokine receptor, Materials science, Nanotechnology, Immunology, Receptor, Inflammation, Biochemistry, Genetics, Mathematics, Mathematical analysisTop concepts (fields/topics) attached by OpenAlex
- Cited by
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8Total citation count in OpenAlex
- Citations by year (recent)
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2025: 3, 2024: 3, 2023: 2Per-year citation counts (last 5 years)
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60Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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