Ablation of Lsd1 induces viral mimicry in thymocytes and promotes the development of innate-memory T cells Article Swipe
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· 2022
· Open Access
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· DOI: https://doi.org/10.21203/rs.3.rs-1961347/v1
· OA: W4307030456
<title>Abstract</title> Histone demethylase Lsd1 has been shown to play a critical role in hematopoietic differentiation. However, its physiological functions in thymocyte development remain elusive. We observed that the specific deletion of <italic>Lsd1</italic> in thymocytes at the double-negative stage causes significant thymic atrophy and reduces peripheral T cells with impaired proliferation capacity. Single-cell RNA-sequencing (scRNA-seq) combined with strand-specific total RNA-seq and ChIP-seq analysis revealed that ablation of <italic>Lsd1</italic> in T cell precursors led to the aberrant de-repression of endogenous retroelements (EREs), which then resulted in a viral mimicry state and activated the interferon pathway. Furthermore, deletion of <italic>Lsd1</italic> blocked the programmed sequential down-regulation of CD8 expression at the DP→CD4<sup>+</sup>CD8<sup>lo</sup> stage and induced an innate-memory phenotype in both thymic and peripheral T cells. Overall, our study provides new insight into the function of Lsd1 as an important maintainer of ERE homeostasis in early T cell development.
