Absorption Spectra as Predictors of Algal Biomass and Pigment Content of the Cultured Microalgae Amphidinium carterae, Isochrysis galbana, Nephroselmis sp., and Anabaena sp. Article Swipe
George N. Hotos
,
Vlasoula Bekiari
·
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.3390/ijpb14040065
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.3390/ijpb14040065
Background: In the search of a rapid and representative method for the approximate calculation of culture density and cell content of useful pigments, the study of absorption spectra of cultures of the dinoflagellate Amphidinium carterae, the haptophyte Isochrysis galbana, the chlorophyte Nephroselmis sp., and the filamentous cyanobacterium Anabaena sp. were selected as representative species of different taxa. Methods: The experimental cultures were established in small volumes by the discontinuous method under 20–21 °C, salinity of 30 or 40 ppt, and 2000 or 8000 lux illumination, and the absorbance spectra, density of the culture and concentration of the extracted pigments chlorophyll, total carotenoids, and phycocyanin were recorded during cultivation. Results: Algal density can be predicted sufficiently correctly because the regression equation of the correlation of the OD value of 750 nm from each absorption spectrum and the measured algal biomass was very strong. The same is true for the corresponding correlations between OD 750 nm and the detected pigments. Conclusions: Absorption spectra of microalgal cultures can be a simple, inexpensive, and non-invasive method for the growers to obtain the necessary information for predicting the right time to collect an ideal combination of maximum biomass and useful pigments, provided that the interpretation of the spectra is performed according to the method described herein.
Related Topics
Concepts
Haptophyte
Isochrysis galbana
Absorbance
Phycocyanin
Botany
Pigment
Biology
Algae
Biomass (ecology)
Chlorophyll a
Carotenoid
Absorption (acoustics)
Salinity
Food science
Chemistry
Chromatography
Cyanobacteria
Phytoplankton
Ecology
Materials science
Bacteria
Composite material
Nutrient
Organic chemistry
Genetics
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3390/ijpb14040065
- https://www.mdpi.com/2037-0164/14/4/65/pdf?version=1695718525
- OA Status
- gold
- Cited By
- 5
- References
- 41
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4387060349
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4387060349Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.3390/ijpb14040065Digital Object Identifier
- Title
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Absorption Spectra as Predictors of Algal Biomass and Pigment Content of the Cultured Microalgae Amphidinium carterae, Isochrysis galbana, Nephroselmis sp., and Anabaena sp.Work title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2023Year of publication
- Publication date
-
2023-09-26Full publication date if available
- Authors
-
George N. Hotos, Vlasoula BekiariList of authors in order
- Landing page
-
https://doi.org/10.3390/ijpb14040065Publisher landing page
- PDF URL
-
https://www.mdpi.com/2037-0164/14/4/65/pdf?version=1695718525Direct link to full text PDF
- Open access
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YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
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https://www.mdpi.com/2037-0164/14/4/65/pdf?version=1695718525Direct OA link when available
- Concepts
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Haptophyte, Isochrysis galbana, Absorbance, Phycocyanin, Botany, Pigment, Biology, Algae, Biomass (ecology), Chlorophyll a, Carotenoid, Absorption (acoustics), Salinity, Food science, Chemistry, Chromatography, Cyanobacteria, Phytoplankton, Ecology, Materials science, Bacteria, Composite material, Nutrient, Organic chemistry, GeneticsTop concepts (fields/topics) attached by OpenAlex
- Cited by
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5Total citation count in OpenAlex
- Citations by year (recent)
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2025: 3, 2024: 2Per-year citation counts (last 5 years)
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41Number of works referenced by this work
- Related works (count)
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10Other works algorithmically related by OpenAlex
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