Acute Oral Toxicity Study of Exapar Premix in Wistar Rats: A Polyherbal Formulation for Uterine Cleansing and Restoration in Veterinary Practice Article Swipe

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Vaidya M.G. , Mistu Tripura , Damekar Soundarya , Giri S. S. , Kamdi B. P. , Gupta S , Hajare S.W ·

YOU? · · 2025 · Open Access · · DOI: https://doi.org/10.9734/jsrr/2025/v31i113711

Aims: The present study evaluated the acute oral toxicity potential of Exapar Premix in Wistar rats following the OECD-423 and 425 guidelines. Study Design: Acute oral toxicity study based on a single-dose administration protocol. Place and Duration of Study: The study was conducted at the Laboratory Animal Facility, Department of Veterinary Pharmacology and Toxicology, Post Graduate Institute of Veterinary and Animal Sciences (PGIVAS), Akola, Maharashtra, India (MAFSU, Nagpur). The experiment was carried out in a controlled laboratory setting over a 14-day observation period, preceded by a five-day acclimatization phase. Methodology: Experiment was conducted on six adults female Wistar rats (108–123 g). The Exapar Premix was administered orally to separate single rat @ 300 mg/kg and 2000 mg/kg body weight in a sighting study and in main test 4 rats were taken separately received 2000 mg/kg of product orally. The animals were continuously monitored for clinical signs of toxicity, behavioral changes and mortality. Body weight was recorded during the 14-day period. On the last day of experiment, biochemical parameters including aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and creatinine levels, were analyzed. The rats were then humanely euthanized using diethyl ether and histopathological examinations of the heart, liver, kidneys and lungs were performed to examine any microscopic abnormalities. Results: No mortality or adverse clinical signs were observed in any of the treated animals. Body weight remained within the normal physiological range throughout the study. The estimated biochemical analytes AST, ALT, ALP and creatinine were observed within normal reference ranges, indicating no systemic toxicity. Histopathological examination showed no significant microscopic abnormalities in the examined vital organs. Conclusion: Exapar Premix upon oral administration do not cause acute oral toxicity in Wistar rats, with an LD₅₀ exceeding 2000 mg/kg body weight. These findings confirm its safety for oral administration.

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Medicine Creatinine Alkaline phosphatase Toxicity Physiology Adverse effect Acute toxicity Body weight Oral administration Alanine aminotransferase Veterinary medicine Histopathological examination Internal medicine Blood chemistry Pharmacology Histopathology Urinalysis No-observed-adverse-effect level Reference range Oral dose Veterinary pathology Pathology Surgery Median lethal dose

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Title: Acute Oral Toxicity Study of Exapar Premix in Wistar Rats: A Polyherbal Formulation for Uterine Cleansing and Restoration in Veterinary Practice

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Publication year: 2025

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Publication date: 2025-11-14

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Authors: Vaidya M.G., Mistu Tripura, Damekar Soundarya, Giri S. S., Kamdi B. P., Gupta S, Hajare S.W

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Concepts: Medicine, Creatinine, Alkaline phosphatase, Toxicity, Physiology, Adverse effect, Acute toxicity, Body weight, Oral administration, Alanine aminotransferase, Veterinary medicine, Histopathological examination, Internal medicine, Blood chemistry, Pharmacology, Histopathology, Urinalysis, No-observed-adverse-effect level, Reference range, Oral dose, Veterinary pathology, Pathology, Surgery, Median lethal dose

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abstract_inverted_index.These	289
abstract_inverted_index.acute	6, 275
abstract_inverted_index.based	28
abstract_inverted_index.cause	274
abstract_inverted_index.ether	191
abstract_inverted_index.lungs	201
abstract_inverted_index.mg/kg	113, 116, 134, 286
abstract_inverted_index.range	231
abstract_inverted_index.rats,	280
abstract_inverted_index.signs	145, 215
abstract_inverted_index.study	3, 27, 40, 122
abstract_inverted_index.taken	130
abstract_inverted_index.using	189
abstract_inverted_index.vital	264
abstract_inverted_index.(ALT),	174
abstract_inverted_index.(AST),	171
abstract_inverted_index.14-day	80, 158
abstract_inverted_index.Akola,	63
abstract_inverted_index.Animal	46, 60
abstract_inverted_index.Exapar	11, 102, 267
abstract_inverted_index.Premix	12, 103, 268
abstract_inverted_index.Study:	38
abstract_inverted_index.Wistar	14, 97, 279
abstract_inverted_index.adults	95
abstract_inverted_index.during	156
abstract_inverted_index.female	96
abstract_inverted_index.heart,	197
abstract_inverted_index.liver,	198
abstract_inverted_index.normal	229, 247
abstract_inverted_index.orally	106
abstract_inverted_index.phase.	88
abstract_inverted_index.safety	293
abstract_inverted_index.showed	256
abstract_inverted_index.single	109
abstract_inverted_index.study.	234
abstract_inverted_index.weight	118, 153, 225
abstract_inverted_index.within	227, 246
abstract_inverted_index.(MAFSU,	66
abstract_inverted_index.Design:	23
abstract_inverted_index.adverse	213
abstract_inverted_index.alanine	172
abstract_inverted_index.animals	139
abstract_inverted_index.carried	71
abstract_inverted_index.changes	149
abstract_inverted_index.confirm	291
abstract_inverted_index.diethyl	190
abstract_inverted_index.examine	205
abstract_inverted_index.kidneys	199
abstract_inverted_index.levels,	180
abstract_inverted_index.orally.	137
abstract_inverted_index.organs.	265
abstract_inverted_index.period,	82
abstract_inverted_index.period.	159
abstract_inverted_index.present	2
abstract_inverted_index.product	136
abstract_inverted_index.ranges,	249
abstract_inverted_index.setting	77
abstract_inverted_index.treated	222
abstract_inverted_index.weight.	288
abstract_inverted_index.Duration	36
abstract_inverted_index.Graduate	55
abstract_inverted_index.LD₅₀	283
abstract_inverted_index.Nagpur).	67
abstract_inverted_index.OECD-423	18
abstract_inverted_index.Results:	209
abstract_inverted_index.Sciences	61
abstract_inverted_index.alkaline	175
abstract_inverted_index.analytes	238
abstract_inverted_index.animals.	223
abstract_inverted_index.clinical	144, 214
abstract_inverted_index.examined	263
abstract_inverted_index.findings	290
abstract_inverted_index.five-day	86
abstract_inverted_index.humanely	187
abstract_inverted_index.observed	217, 245
abstract_inverted_index.preceded	83
abstract_inverted_index.received	132
abstract_inverted_index.recorded	155
abstract_inverted_index.remained	226
abstract_inverted_index.separate	108
abstract_inverted_index.sighting	121
abstract_inverted_index.systemic	252
abstract_inverted_index.toxicity	8, 26, 277
abstract_inverted_index.(PGIVAS),	62
abstract_inverted_index.Facility,	47
abstract_inverted_index.Institute	56
abstract_inverted_index.analyzed.	182
abstract_inverted_index.aspartate	169
abstract_inverted_index.conducted	42, 92
abstract_inverted_index.estimated	236
abstract_inverted_index.evaluated	4
abstract_inverted_index.exceeding	284
abstract_inverted_index.following	16
abstract_inverted_index.including	168
abstract_inverted_index.monitored	142
abstract_inverted_index.mortality	211
abstract_inverted_index.performed	203
abstract_inverted_index.potential	9
abstract_inverted_index.protocol.	33
abstract_inverted_index.reference	248
abstract_inverted_index.toxicity,	147
abstract_inverted_index.toxicity.	253
abstract_inverted_index.(108–123	99
abstract_inverted_index.Department	48
abstract_inverted_index.Experiment	90
abstract_inverted_index.Laboratory	45
abstract_inverted_index.Veterinary	50, 58
abstract_inverted_index.behavioral	148
abstract_inverted_index.controlled	75
abstract_inverted_index.creatinine	179, 243
abstract_inverted_index.euthanized	188
abstract_inverted_index.experiment	69
abstract_inverted_index.indicating	250
abstract_inverted_index.laboratory	76
abstract_inverted_index.mortality.	151
abstract_inverted_index.parameters	167
abstract_inverted_index.separately	131
abstract_inverted_index.throughout	232
abstract_inverted_index.Conclusion:	266
abstract_inverted_index.Toxicology,	53
abstract_inverted_index.biochemical	166, 237
abstract_inverted_index.examination	255
abstract_inverted_index.experiment,	165
abstract_inverted_index.guidelines.	21
abstract_inverted_index.microscopic	207, 259
abstract_inverted_index.observation	81
abstract_inverted_index.phosphatase	176
abstract_inverted_index.significant	258
abstract_inverted_index.single-dose	31
abstract_inverted_index.Maharashtra,	64
abstract_inverted_index.Methodology:	89
abstract_inverted_index.Pharmacology	51
abstract_inverted_index.administered	105
abstract_inverted_index.continuously	141
abstract_inverted_index.examinations	194
abstract_inverted_index.abnormalities	260
abstract_inverted_index.physiological	230
abstract_inverted_index.abnormalities.	208
abstract_inverted_index.administration	32, 271
abstract_inverted_index.acclimatization	87
abstract_inverted_index.administration.	296
abstract_inverted_index.aminotransferase	170, 173
abstract_inverted_index.Histopathological	254
abstract_inverted_index.histopathological	193
cited_by_percentile_year
countries_distinct_count	0
institutions_distinct_count	7
citation_normalized_percentile.value	0.74255608
citation_normalized_percentile.is_in_top_1_percent	False
citation_normalized_percentile.is_in_top_10_percent	False