Alternative Culture Systems for Bovine Oocyte In Vitro Maturation: Liquid Marbles and Differentially Shaped 96-Well Plates Article Swipe
Andrea Fernández-Montoro
,
Daniel Angel‐Velez
,
Camilla Benedetti
,
Nima Azari‐Dolatabad
,
Osvaldo Bogado Pascottini
,
Ann Van Soom
,
Krishna Chaitanya Pavani
·
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.3390/ani13101635
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.3390/ani13101635
In vivo-matured oocytes exhibit higher developmental competence than those matured in vitro but mimicking the in vivo environment by in vitro conditions has been challenging. Until now, conventional two-dimensional (2D) systems have been used for in vitro maturation of bovine cumulus-oocytes-complexes (COCs). However, using such systems present certain limitations. Therefore, alternative low-cost methodologies may help to optimize oocyte in vitro maturation. Here, we used two different systems to culture COCs and evaluate their potential influence on embryo development and quality. In the first system, we used treated fumed silica particles to create a 3D microenvironment (liquid marbles; LM) to mature COCs. In the second system, we cultured COCs in 96-well plates with different dimensions (flat, ultra-low attachment round-bottom, and v-shaped 96-well plates). In both systems, the nuclear maturation rate remained similar to the control in 2D, showing that most oocytes reached metaphase II. However, the subsequent blastocyst rate remained lower in the liquid marble system compared with the 96-well plates and control 2D systems. Interestingly, a lower total cell number was found in the resulting embryos from both systems (LM and 96-well plates) compared with the control. In conclusion, oocytes matured in liquid marbles or 96-well plates showed no remarkable change in terms of meiotic resumption. None of the surface geometries influenced embryo development while oocyte maturation in liquid marbles led to reduced embryo development. These findings show that different geometry during maturation did not have a large impact on oocyte and embryo development. Lower embryo production after in vitro maturation in liquid marbles was probably detected because in vitro maturation was performed in serum-free medium, which makes oocytes more sensitive to possible toxic effects from the environment.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3390/ani13101635
- https://www.mdpi.com/2076-2615/13/10/1635/pdf?version=1684381273
- OA Status
- gold
- Cited By
- 4
- References
- 57
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4378515964
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4378515964Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.3390/ani13101635Digital Object Identifier
- Title
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Alternative Culture Systems for Bovine Oocyte In Vitro Maturation: Liquid Marbles and Differentially Shaped 96-Well PlatesWork title
- Type
-
articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2023Year of publication
- Publication date
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2023-05-14Full publication date if available
- Authors
-
Andrea Fernández-Montoro, Daniel Angel‐Velez, Camilla Benedetti, Nima Azari‐Dolatabad, Osvaldo Bogado Pascottini, Ann Van Soom, Krishna Chaitanya PavaniList of authors in order
- Landing page
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https://doi.org/10.3390/ani13101635Publisher landing page
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https://www.mdpi.com/2076-2615/13/10/1635/pdf?version=1684381273Direct link to full text PDF
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YesWhether a free full text is available
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goldOpen access status per OpenAlex
- OA URL
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https://www.mdpi.com/2076-2615/13/10/1635/pdf?version=1684381273Direct OA link when available
- Concepts
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Oocyte, Blastocyst, In vitro maturation, Embryo, In vitro, Andrology, In vivo, Embryogenesis, Cell biology, Biology, Chemistry, Genetics, MedicineTop concepts (fields/topics) attached by OpenAlex
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4Total citation count in OpenAlex
- Citations by year (recent)
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2025: 3, 2024: 1Per-year citation counts (last 5 years)
- References (count)
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57Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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