Amplicon sequencing with internal standards yields accurate picocyanobacteria cell abundances as validated with flow cytometry Article Swipe
Alexandra E. Jones-Kellett
,
Jesse McNichol
,
Yubin Raut
,
Kelsy Cain
,
François Ribalet
,
E. Virginia Armbrust
,
Michael J. Follows
,
Jed A. Fuhrman
·
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1093/ismeco/ycae115
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1093/ismeco/ycae115
To understand ecosystem state and function, marine microbial ecologists seek measurements of organismal abundance and diversity at high taxonomic resolution. Conventional flow cytometry accurately estimates microbial cell abundance but only discerns broad groups with distinct optical properties. While amplicon sequencing resolves more comprehensive diversity within microbiomes, it typically only provides relative organismal abundances within samples, not absolute abundance changes. Internal genomic standards offer a solution for absolute amplicon-based measures. Here, we spiked genomic standards into plankton samples from surface seawater, gathered at 46-km intervals along a cruise transect spanning the southern California Current System and the oligotrophic North Pacific Subtropical Gyre. This enabled evaluation of the absolute volumetric gene copy abundances of 16S rRNA amplicon sequence variants (amplified with 515Y-926R universal primers, quantitatively validated with mock communities) and cell abundances of picocyanobacteria with known genomic 16S copy numbers. Comparison of amplicon-derived cell abundances of Prochlorococcus and Synechococcus with flow cytometry data from nearby locations yielded nearly identical results (slope = 1.01; Pearson’s r = 0.9942). Our findings show that this amplicon sequencing protocol combined with genomic internal standards accurately measures absolute cell counts of marine picocyanobacteria in complex field samples. By extension, we expect this approach to reasonably estimate volumetric gene copies for other amplified taxa in these samples.
Related Topics
Concepts
Prochlorococcus
Amplicon
Biology
Pyrosequencing
Synechococcus
Relative species abundance
Abundance (ecology)
Ecology
Genetics
Gene
Polymerase chain reaction
Cyanobacteria
Bacteria
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1093/ismeco/ycae115
- OA Status
- gold
- Cited By
- 11
- References
- 21
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4402900072
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W4402900072Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.1093/ismeco/ycae115Digital Object Identifier
- Title
-
Amplicon sequencing with internal standards yields accurate picocyanobacteria cell abundances as validated with flow cytometryWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2024Year of publication
- Publication date
-
2024-01-01Full publication date if available
- Authors
-
Alexandra E. Jones-Kellett, Jesse McNichol, Yubin Raut, Kelsy Cain, François Ribalet, E. Virginia Armbrust, Michael J. Follows, Jed A. FuhrmanList of authors in order
- Landing page
-
https://doi.org/10.1093/ismeco/ycae115Publisher landing page
- Open access
-
YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
-
https://doi.org/10.1093/ismeco/ycae115Direct OA link when available
- Concepts
-
Prochlorococcus, Amplicon, Biology, Pyrosequencing, Synechococcus, Relative species abundance, Abundance (ecology), Ecology, Genetics, Gene, Polymerase chain reaction, Cyanobacteria, BacteriaTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
11Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 10, 2024: 1Per-year citation counts (last 5 years)
- References (count)
-
21Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
Full payload
| id | https://openalex.org/W4402900072 |
|---|---|
| doi | https://doi.org/10.1093/ismeco/ycae115 |
| ids.doi | https://doi.org/10.1093/ismeco/ycae115 |
| ids.pmid | https://pubmed.ncbi.nlm.nih.gov/39381325 |
| ids.openalex | https://openalex.org/W4402900072 |
| fwci | 9.67522265 |
| type | article |
| title | Amplicon sequencing with internal standards yields accurate picocyanobacteria cell abundances as validated with flow cytometry |
| awards[0].id | https://openalex.org/G1506428867 |
| awards[0].funder_id | https://openalex.org/F4320306164 |
| awards[0].display_name | |
| awards[0].funder_award_id | 723795 |
| awards[0].funder_display_name | Simons Foundation |
| awards[1].id | https://openalex.org/G3588454487 |
| awards[1].funder_id | https://openalex.org/F4320306164 |
| awards[1].display_name | |
| awards[1].funder_award_id | 549943 |
| awards[1].funder_display_name | Simons Foundation |
| awards[2].id | https://openalex.org/G2077327224 |
| awards[2].funder_id | https://openalex.org/F4320306164 |
| awards[2].display_name | |
| awards[2].funder_award_id | 549931 |
| awards[2].funder_display_name | Simons Foundation |
| awards[3].id | https://openalex.org/G1243517580 |
| awards[3].funder_id | https://openalex.org/F4320306164 |
| awards[3].display_name | |
| awards[3].funder_award_id | 329108 |
| awards[3].funder_display_name | Simons Foundation |
| biblio.issue | 1 |
| biblio.volume | 4 |
| biblio.last_page | ycae115 |
| biblio.first_page | ycae115 |
| topics[0].id | https://openalex.org/T11791 |
| topics[0].field.id | https://openalex.org/fields/23 |
| topics[0].field.display_name | Environmental Science |
| topics[0].score | 0.9997000098228455 |
| topics[0].domain.id | https://openalex.org/domains/3 |
| topics[0].domain.display_name | Physical Sciences |
| topics[0].subfield.id | https://openalex.org/subfields/2303 |
| topics[0].subfield.display_name | Ecology |
| topics[0].display_name | Microbial Community Ecology and Physiology |
| topics[1].id | https://openalex.org/T11879 |
| topics[1].field.id | https://openalex.org/fields/13 |
| topics[1].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[1].score | 0.9970999956130981 |
| topics[1].domain.id | https://openalex.org/domains/1 |
| topics[1].domain.display_name | Life Sciences |
| topics[1].subfield.id | https://openalex.org/subfields/1312 |
| topics[1].subfield.display_name | Molecular Biology |
| topics[1].display_name | Protist diversity and phylogeny |
| topics[2].id | https://openalex.org/T12640 |
| topics[2].field.id | https://openalex.org/fields/23 |
| topics[2].field.display_name | Environmental Science |
| topics[2].score | 0.9854999780654907 |
| topics[2].domain.id | https://openalex.org/domains/3 |
| topics[2].domain.display_name | Physical Sciences |
| topics[2].subfield.id | https://openalex.org/subfields/2303 |
| topics[2].subfield.display_name | Ecology |
| topics[2].display_name | Environmental DNA in Biodiversity Studies |
| funders[0].id | https://openalex.org/F4320306164 |
| funders[0].ror | https://ror.org/01cmst727 |
| funders[0].display_name | Simons Foundation |
| is_xpac | False |
| apc_list.value | 1690 |
| apc_list.currency | GBP |
| apc_list.value_usd | 2072 |
| apc_paid.value | 1690 |
| apc_paid.currency | GBP |
| apc_paid.value_usd | 2072 |
| concepts[0].id | https://openalex.org/C2779207893 |
| concepts[0].level | 5 |
| concepts[0].score | 0.8830496072769165 |
| concepts[0].wikidata | https://www.wikidata.org/wiki/Q18645284 |
| concepts[0].display_name | Prochlorococcus |
| concepts[1].id | https://openalex.org/C8185291 |
| concepts[1].level | 4 |
| concepts[1].score | 0.8103621006011963 |
| concepts[1].wikidata | https://www.wikidata.org/wiki/Q2844457 |
| concepts[1].display_name | Amplicon |
| concepts[2].id | https://openalex.org/C86803240 |
| concepts[2].level | 0 |
| concepts[2].score | 0.6796186566352844 |
| concepts[2].wikidata | https://www.wikidata.org/wiki/Q420 |
| concepts[2].display_name | Biology |
| concepts[3].id | https://openalex.org/C125009961 |
| concepts[3].level | 3 |
| concepts[3].score | 0.5806530117988586 |
| concepts[3].wikidata | https://www.wikidata.org/wiki/Q2342248 |
| concepts[3].display_name | Pyrosequencing |
| concepts[4].id | https://openalex.org/C2777219426 |
| concepts[4].level | 4 |
| concepts[4].score | 0.5493922829627991 |
| concepts[4].wikidata | https://www.wikidata.org/wiki/Q150962 |
| concepts[4].display_name | Synechococcus |
| concepts[5].id | https://openalex.org/C122325731 |
| concepts[5].level | 3 |
| concepts[5].score | 0.46207621693611145 |
| concepts[5].wikidata | https://www.wikidata.org/wiki/Q3738459 |
| concepts[5].display_name | Relative species abundance |
| concepts[6].id | https://openalex.org/C77077793 |
| concepts[6].level | 2 |
| concepts[6].score | 0.44421839714050293 |
| concepts[6].wikidata | https://www.wikidata.org/wiki/Q336019 |
| concepts[6].display_name | Abundance (ecology) |
| concepts[7].id | https://openalex.org/C18903297 |
| concepts[7].level | 1 |
| concepts[7].score | 0.2840169668197632 |
| concepts[7].wikidata | https://www.wikidata.org/wiki/Q7150 |
| concepts[7].display_name | Ecology |
| concepts[8].id | https://openalex.org/C54355233 |
| concepts[8].level | 1 |
| concepts[8].score | 0.2598487436771393 |
| concepts[8].wikidata | https://www.wikidata.org/wiki/Q7162 |
| concepts[8].display_name | Genetics |
| concepts[9].id | https://openalex.org/C104317684 |
| concepts[9].level | 2 |
| concepts[9].score | 0.2235097587108612 |
| concepts[9].wikidata | https://www.wikidata.org/wiki/Q7187 |
| concepts[9].display_name | Gene |
| concepts[10].id | https://openalex.org/C49105822 |
| concepts[10].level | 3 |
| concepts[10].score | 0.2227085530757904 |
| concepts[10].wikidata | https://www.wikidata.org/wiki/Q176996 |
| concepts[10].display_name | Polymerase chain reaction |
| concepts[11].id | https://openalex.org/C2779669040 |
| concepts[11].level | 3 |
| concepts[11].score | 0.20383545756340027 |
| concepts[11].wikidata | https://www.wikidata.org/wiki/Q93315 |
| concepts[11].display_name | Cyanobacteria |
| concepts[12].id | https://openalex.org/C523546767 |
| concepts[12].level | 2 |
| concepts[12].score | 0.08938133716583252 |
| concepts[12].wikidata | https://www.wikidata.org/wiki/Q10876 |
| concepts[12].display_name | Bacteria |
| keywords[0].id | https://openalex.org/keywords/prochlorococcus |
| keywords[0].score | 0.8830496072769165 |
| keywords[0].display_name | Prochlorococcus |
| keywords[1].id | https://openalex.org/keywords/amplicon |
| keywords[1].score | 0.8103621006011963 |
| keywords[1].display_name | Amplicon |
| keywords[2].id | https://openalex.org/keywords/biology |
| keywords[2].score | 0.6796186566352844 |
| keywords[2].display_name | Biology |
| keywords[3].id | https://openalex.org/keywords/pyrosequencing |
| keywords[3].score | 0.5806530117988586 |
| keywords[3].display_name | Pyrosequencing |
| keywords[4].id | https://openalex.org/keywords/synechococcus |
| keywords[4].score | 0.5493922829627991 |
| keywords[4].display_name | Synechococcus |
| keywords[5].id | https://openalex.org/keywords/relative-species-abundance |
| keywords[5].score | 0.46207621693611145 |
| keywords[5].display_name | Relative species abundance |
| keywords[6].id | https://openalex.org/keywords/abundance |
| keywords[6].score | 0.44421839714050293 |
| keywords[6].display_name | Abundance (ecology) |
| keywords[7].id | https://openalex.org/keywords/ecology |
| keywords[7].score | 0.2840169668197632 |
| keywords[7].display_name | Ecology |
| keywords[8].id | https://openalex.org/keywords/genetics |
| keywords[8].score | 0.2598487436771393 |
| keywords[8].display_name | Genetics |
| keywords[9].id | https://openalex.org/keywords/gene |
| keywords[9].score | 0.2235097587108612 |
| keywords[9].display_name | Gene |
| keywords[10].id | https://openalex.org/keywords/polymerase-chain-reaction |
| keywords[10].score | 0.2227085530757904 |
| keywords[10].display_name | Polymerase chain reaction |
| keywords[11].id | https://openalex.org/keywords/cyanobacteria |
| keywords[11].score | 0.20383545756340027 |
| keywords[11].display_name | Cyanobacteria |
| keywords[12].id | https://openalex.org/keywords/bacteria |
| keywords[12].score | 0.08938133716583252 |
| keywords[12].display_name | Bacteria |
| language | en |
| locations[0].id | doi:10.1093/ismeco/ycae115 |
| locations[0].is_oa | True |
| locations[0].source.id | https://openalex.org/S4210240812 |
| locations[0].source.issn | 2730-6151 |
| locations[0].source.type | journal |
| locations[0].source.is_oa | True |
| locations[0].source.issn_l | 2730-6151 |
| locations[0].source.is_core | True |
| locations[0].source.is_in_doaj | True |
| locations[0].source.display_name | ISME Communications |
| locations[0].source.host_organization | https://openalex.org/P4310319965 |
| locations[0].source.host_organization_name | Springer Nature |
| locations[0].source.host_organization_lineage | https://openalex.org/P4310319965 |
| locations[0].source.host_organization_lineage_names | Springer Nature |
| locations[0].license | cc-by |
| locations[0].pdf_url | |
| locations[0].version | publishedVersion |
| locations[0].raw_type | journal-article |
| locations[0].license_id | https://openalex.org/licenses/cc-by |
| locations[0].is_accepted | True |
| locations[0].is_published | True |
| locations[0].raw_source_name | ISME Communications |
| locations[0].landing_page_url | https://doi.org/10.1093/ismeco/ycae115 |
| locations[1].id | pmid:39381325 |
| locations[1].is_oa | False |
| locations[1].source.id | https://openalex.org/S4306525036 |
| locations[1].source.issn | |
| locations[1].source.type | repository |
| locations[1].source.is_oa | False |
| locations[1].source.issn_l | |
| locations[1].source.is_core | False |
| locations[1].source.is_in_doaj | False |
| locations[1].source.display_name | PubMed |
| locations[1].source.host_organization | https://openalex.org/I1299303238 |
| locations[1].source.host_organization_name | National Institutes of Health |
| locations[1].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[1].license | |
| locations[1].pdf_url | |
| locations[1].version | publishedVersion |
| locations[1].raw_type | |
| locations[1].license_id | |
| locations[1].is_accepted | True |
| locations[1].is_published | True |
| locations[1].raw_source_name | ISME communications |
| locations[1].landing_page_url | https://pubmed.ncbi.nlm.nih.gov/39381325 |
| locations[2].id | pmh:oai:pubmedcentral.nih.gov:11459381 |
| locations[2].is_oa | True |
| locations[2].source.id | https://openalex.org/S2764455111 |
| locations[2].source.issn | |
| locations[2].source.type | repository |
| locations[2].source.is_oa | False |
| locations[2].source.issn_l | |
| locations[2].source.is_core | False |
| locations[2].source.is_in_doaj | False |
| locations[2].source.display_name | PubMed Central |
| locations[2].source.host_organization | https://openalex.org/I1299303238 |
| locations[2].source.host_organization_name | National Institutes of Health |
| locations[2].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[2].license | other-oa |
| locations[2].pdf_url | |
| locations[2].version | submittedVersion |
| locations[2].raw_type | Text |
| locations[2].license_id | https://openalex.org/licenses/other-oa |
| locations[2].is_accepted | False |
| locations[2].is_published | False |
| locations[2].raw_source_name | ISME Commun |
| locations[2].landing_page_url | https://www.ncbi.nlm.nih.gov/pmc/articles/11459381 |
| indexed_in | crossref, doaj, pubmed |
| authorships[0].author.id | https://openalex.org/A5093131584 |
| authorships[0].author.orcid | https://orcid.org/0000-0002-1727-2219 |
| authorships[0].author.display_name | Alexandra E. Jones-Kellett |
| authorships[0].countries | US |
| authorships[0].affiliations[0].institution_ids | https://openalex.org/I66958751 |
| authorships[0].affiliations[0].raw_affiliation_string | Biology Department, Woods Hole Oceanographic Institution , Woods Hole, MA 02543, |
| authorships[0].affiliations[1].institution_ids | https://openalex.org/I1302918504 |
| authorships[0].affiliations[1].raw_affiliation_string | Department of Earth , Atmospheric, and Planetary Sciences, , Cambridge, MA 02139, |
| authorships[0].affiliations[2].institution_ids | https://openalex.org/I63966007 |
| authorships[0].affiliations[2].raw_affiliation_string | Massachusetts Institute of Technology , Atmospheric, and Planetary Sciences, , Cambridge, MA 02139, |
| authorships[0].institutions[0].id | https://openalex.org/I63966007 |
| authorships[0].institutions[0].ror | https://ror.org/042nb2s44 |
| authorships[0].institutions[0].type | education |
| authorships[0].institutions[0].lineage | https://openalex.org/I63966007 |
| authorships[0].institutions[0].country_code | US |
| authorships[0].institutions[0].display_name | Massachusetts Institute of Technology |
| authorships[0].institutions[1].id | https://openalex.org/I1302918504 |
| authorships[0].institutions[1].ror | https://ror.org/05vvg9554 |
| authorships[0].institutions[1].type | nonprofit |
| authorships[0].institutions[1].lineage | https://openalex.org/I1302918504 |
| authorships[0].institutions[1].country_code | US |
| authorships[0].institutions[1].display_name | Planetary Science Institute |
| authorships[0].institutions[2].id | https://openalex.org/I66958751 |
| authorships[0].institutions[2].ror | https://ror.org/03zbnzt98 |
| authorships[0].institutions[2].type | nonprofit |
| authorships[0].institutions[2].lineage | https://openalex.org/I66958751 |
| authorships[0].institutions[2].country_code | US |
| authorships[0].institutions[2].display_name | Woods Hole Oceanographic Institution |
| authorships[0].author_position | first |
| authorships[0].raw_author_name | Alexandra E Jones-Kellett |
| authorships[0].is_corresponding | False |
| authorships[0].raw_affiliation_strings | Biology Department, Woods Hole Oceanographic Institution , Woods Hole, MA 02543,, Department of Earth , Atmospheric, and Planetary Sciences, , Cambridge, MA 02139,, Massachusetts Institute of Technology , Atmospheric, and Planetary Sciences, , Cambridge, MA 02139, |
| authorships[1].author.id | https://openalex.org/A5056416989 |
| authorships[1].author.orcid | https://orcid.org/0000-0002-8870-7726 |
| authorships[1].author.display_name | Jesse McNichol |
| authorships[1].countries | CA, US |
| authorships[1].affiliations[0].institution_ids | https://openalex.org/I197191942 |
| authorships[1].affiliations[0].raw_affiliation_string | Biology Department, St. Francis Xavier University , Antigonish, NS B2G 2W5, |
| authorships[1].affiliations[1].institution_ids | https://openalex.org/I1174212 |
| authorships[1].affiliations[1].raw_affiliation_string | Department of Biological Sciences, University of Southern California , Los Angeles, CA 90007, |
| authorships[1].institutions[0].id | https://openalex.org/I197191942 |
| authorships[1].institutions[0].ror | https://ror.org/01wcaxs37 |
| authorships[1].institutions[0].type | education |
| authorships[1].institutions[0].lineage | https://openalex.org/I197191942 |
| authorships[1].institutions[0].country_code | CA |
| authorships[1].institutions[0].display_name | St. Francis Xavier University |
| authorships[1].institutions[1].id | https://openalex.org/I1174212 |
| authorships[1].institutions[1].ror | https://ror.org/03taz7m60 |
| authorships[1].institutions[1].type | education |
| authorships[1].institutions[1].lineage | https://openalex.org/I1174212 |
| authorships[1].institutions[1].country_code | US |
| authorships[1].institutions[1].display_name | University of Southern California |
| authorships[1].author_position | middle |
| authorships[1].raw_author_name | Jesse C McNichol |
| authorships[1].is_corresponding | False |
| authorships[1].raw_affiliation_strings | Biology Department, St. Francis Xavier University , Antigonish, NS B2G 2W5,, Department of Biological Sciences, University of Southern California , Los Angeles, CA 90007, |
| authorships[2].author.id | https://openalex.org/A5084011935 |
| authorships[2].author.orcid | https://orcid.org/0000-0003-3841-7023 |
| authorships[2].author.display_name | Yubin Raut |
| authorships[2].countries | US |
| authorships[2].affiliations[0].institution_ids | https://openalex.org/I1174212 |
| authorships[2].affiliations[0].raw_affiliation_string | Department of Biological Sciences, University of Southern California , Los Angeles, CA 90007, |
| authorships[2].affiliations[1].institution_ids | https://openalex.org/I63966007 |
| authorships[2].affiliations[1].raw_affiliation_string | Massachusetts Institute of Technology , Atmospheric, and Planetary Sciences, , Cambridge, MA 02139, |
| authorships[2].affiliations[2].institution_ids | https://openalex.org/I1302918504 |
| authorships[2].affiliations[2].raw_affiliation_string | Department of Earth , Atmospheric, and Planetary Sciences, , Cambridge, MA 02139, |
| authorships[2].institutions[0].id | https://openalex.org/I63966007 |
| authorships[2].institutions[0].ror | https://ror.org/042nb2s44 |
| authorships[2].institutions[0].type | education |
| authorships[2].institutions[0].lineage | https://openalex.org/I63966007 |
| authorships[2].institutions[0].country_code | US |
| authorships[2].institutions[0].display_name | Massachusetts Institute of Technology |
| authorships[2].institutions[1].id | https://openalex.org/I1302918504 |
| authorships[2].institutions[1].ror | https://ror.org/05vvg9554 |
| authorships[2].institutions[1].type | nonprofit |
| authorships[2].institutions[1].lineage | https://openalex.org/I1302918504 |
| authorships[2].institutions[1].country_code | US |
| authorships[2].institutions[1].display_name | Planetary Science Institute |
| authorships[2].institutions[2].id | https://openalex.org/I1174212 |
| authorships[2].institutions[2].ror | https://ror.org/03taz7m60 |
| authorships[2].institutions[2].type | education |
| authorships[2].institutions[2].lineage | https://openalex.org/I1174212 |
| authorships[2].institutions[2].country_code | US |
| authorships[2].institutions[2].display_name | University of Southern California |
| authorships[2].author_position | middle |
| authorships[2].raw_author_name | Yubin Raut |
| authorships[2].is_corresponding | False |
| authorships[2].raw_affiliation_strings | Department of Biological Sciences, University of Southern California , Los Angeles, CA 90007,, Department of Earth , Atmospheric, and Planetary Sciences, , Cambridge, MA 02139,, Massachusetts Institute of Technology , Atmospheric, and Planetary Sciences, , Cambridge, MA 02139, |
| authorships[3].author.id | https://openalex.org/A5039591190 |
| authorships[3].author.orcid | https://orcid.org/0000-0001-8567-3340 |
| authorships[3].author.display_name | Kelsy Cain |
| authorships[3].countries | US |
| authorships[3].affiliations[0].institution_ids | https://openalex.org/I201448701 |
| authorships[3].affiliations[0].raw_affiliation_string | School of Oceanography, University of Washington , Seattle, WA 98195, |
| authorships[3].institutions[0].id | https://openalex.org/I201448701 |
| authorships[3].institutions[0].ror | https://ror.org/00cvxb145 |
| authorships[3].institutions[0].type | education |
| authorships[3].institutions[0].lineage | https://openalex.org/I201448701 |
| authorships[3].institutions[0].country_code | US |
| authorships[3].institutions[0].display_name | University of Washington |
| authorships[3].author_position | middle |
| authorships[3].raw_author_name | Kelsy R Cain |
| authorships[3].is_corresponding | False |
| authorships[3].raw_affiliation_strings | School of Oceanography, University of Washington , Seattle, WA 98195, |
| authorships[4].author.id | https://openalex.org/A5044515635 |
| authorships[4].author.orcid | https://orcid.org/0000-0002-7431-0234 |
| authorships[4].author.display_name | François Ribalet |
| authorships[4].countries | US |
| authorships[4].affiliations[0].institution_ids | https://openalex.org/I201448701 |
| authorships[4].affiliations[0].raw_affiliation_string | School of Oceanography, University of Washington , Seattle, WA 98195, |
| authorships[4].institutions[0].id | https://openalex.org/I201448701 |
| authorships[4].institutions[0].ror | https://ror.org/00cvxb145 |
| authorships[4].institutions[0].type | education |
| authorships[4].institutions[0].lineage | https://openalex.org/I201448701 |
| authorships[4].institutions[0].country_code | US |
| authorships[4].institutions[0].display_name | University of Washington |
| authorships[4].author_position | middle |
| authorships[4].raw_author_name | François Ribalet |
| authorships[4].is_corresponding | False |
| authorships[4].raw_affiliation_strings | School of Oceanography, University of Washington , Seattle, WA 98195, |
| authorships[5].author.id | https://openalex.org/A5087975584 |
| authorships[5].author.orcid | https://orcid.org/0000-0001-7865-5101 |
| authorships[5].author.display_name | E. Virginia Armbrust |
| authorships[5].countries | US |
| authorships[5].affiliations[0].institution_ids | https://openalex.org/I201448701 |
| authorships[5].affiliations[0].raw_affiliation_string | School of Oceanography, University of Washington , Seattle, WA 98195, |
| authorships[5].institutions[0].id | https://openalex.org/I201448701 |
| authorships[5].institutions[0].ror | https://ror.org/00cvxb145 |
| authorships[5].institutions[0].type | education |
| authorships[5].institutions[0].lineage | https://openalex.org/I201448701 |
| authorships[5].institutions[0].country_code | US |
| authorships[5].institutions[0].display_name | University of Washington |
| authorships[5].author_position | middle |
| authorships[5].raw_author_name | E Virginia Armbrust |
| authorships[5].is_corresponding | False |
| authorships[5].raw_affiliation_strings | School of Oceanography, University of Washington , Seattle, WA 98195, |
| authorships[6].author.id | https://openalex.org/A5081551197 |
| authorships[6].author.orcid | https://orcid.org/0000-0002-3102-0341 |
| authorships[6].author.display_name | Michael J. Follows |
| authorships[6].countries | US |
| authorships[6].affiliations[0].institution_ids | https://openalex.org/I63966007 |
| authorships[6].affiliations[0].raw_affiliation_string | Massachusetts Institute of Technology , Atmospheric, and Planetary Sciences, , Cambridge, MA 02139, |
| authorships[6].affiliations[1].institution_ids | https://openalex.org/I1302918504 |
| authorships[6].affiliations[1].raw_affiliation_string | Department of Earth , Atmospheric, and Planetary Sciences, , Cambridge, MA 02139, |
| authorships[6].institutions[0].id | https://openalex.org/I63966007 |
| authorships[6].institutions[0].ror | https://ror.org/042nb2s44 |
| authorships[6].institutions[0].type | education |
| authorships[6].institutions[0].lineage | https://openalex.org/I63966007 |
| authorships[6].institutions[0].country_code | US |
| authorships[6].institutions[0].display_name | Massachusetts Institute of Technology |
| authorships[6].institutions[1].id | https://openalex.org/I1302918504 |
| authorships[6].institutions[1].ror | https://ror.org/05vvg9554 |
| authorships[6].institutions[1].type | nonprofit |
| authorships[6].institutions[1].lineage | https://openalex.org/I1302918504 |
| authorships[6].institutions[1].country_code | US |
| authorships[6].institutions[1].display_name | Planetary Science Institute |
| authorships[6].author_position | middle |
| authorships[6].raw_author_name | Michael J Follows |
| authorships[6].is_corresponding | False |
| authorships[6].raw_affiliation_strings | Department of Earth , Atmospheric, and Planetary Sciences, , Cambridge, MA 02139,, Massachusetts Institute of Technology , Atmospheric, and Planetary Sciences, , Cambridge, MA 02139, |
| authorships[7].author.id | https://openalex.org/A5035958952 |
| authorships[7].author.orcid | https://orcid.org/0000-0002-2361-1985 |
| authorships[7].author.display_name | Jed A. Fuhrman |
| authorships[7].countries | US |
| authorships[7].affiliations[0].institution_ids | https://openalex.org/I1174212 |
| authorships[7].affiliations[0].raw_affiliation_string | Department of Biological Sciences, University of Southern California , Los Angeles, CA 90007, |
| authorships[7].institutions[0].id | https://openalex.org/I1174212 |
| authorships[7].institutions[0].ror | https://ror.org/03taz7m60 |
| authorships[7].institutions[0].type | education |
| authorships[7].institutions[0].lineage | https://openalex.org/I1174212 |
| authorships[7].institutions[0].country_code | US |
| authorships[7].institutions[0].display_name | University of Southern California |
| authorships[7].author_position | last |
| authorships[7].raw_author_name | Jed A Fuhrman |
| authorships[7].is_corresponding | False |
| authorships[7].raw_affiliation_strings | Department of Biological Sciences, University of Southern California , Los Angeles, CA 90007, |
| has_content.pdf | False |
| has_content.grobid_xml | False |
| is_paratext | False |
| open_access.is_oa | True |
| open_access.oa_url | https://doi.org/10.1093/ismeco/ycae115 |
| open_access.oa_status | gold |
| open_access.any_repository_has_fulltext | False |
| created_date | 2025-10-10T00:00:00 |
| display_name | Amplicon sequencing with internal standards yields accurate picocyanobacteria cell abundances as validated with flow cytometry |
| has_fulltext | False |
| is_retracted | False |
| updated_date | 2025-11-06T03:46:38.306776 |
| primary_topic.id | https://openalex.org/T11791 |
| primary_topic.field.id | https://openalex.org/fields/23 |
| primary_topic.field.display_name | Environmental Science |
| primary_topic.score | 0.9997000098228455 |
| primary_topic.domain.id | https://openalex.org/domains/3 |
| primary_topic.domain.display_name | Physical Sciences |
| primary_topic.subfield.id | https://openalex.org/subfields/2303 |
| primary_topic.subfield.display_name | Ecology |
| primary_topic.display_name | Microbial Community Ecology and Physiology |
| related_works | https://openalex.org/W1978886837, https://openalex.org/W2142021629, https://openalex.org/W3150555100, https://openalex.org/W2028685254, https://openalex.org/W2406277324, https://openalex.org/W2776666304, https://openalex.org/W2085615216, https://openalex.org/W1995233033, https://openalex.org/W2076545793, https://openalex.org/W2094192369 |
| cited_by_count | 11 |
| counts_by_year[0].year | 2025 |
| counts_by_year[0].cited_by_count | 10 |
| counts_by_year[1].year | 2024 |
| counts_by_year[1].cited_by_count | 1 |
| locations_count | 3 |
| best_oa_location.id | doi:10.1093/ismeco/ycae115 |
| best_oa_location.is_oa | True |
| best_oa_location.source.id | https://openalex.org/S4210240812 |
| best_oa_location.source.issn | 2730-6151 |
| best_oa_location.source.type | journal |
| best_oa_location.source.is_oa | True |
| best_oa_location.source.issn_l | 2730-6151 |
| best_oa_location.source.is_core | True |
| best_oa_location.source.is_in_doaj | True |
| best_oa_location.source.display_name | ISME Communications |
| best_oa_location.source.host_organization | https://openalex.org/P4310319965 |
| best_oa_location.source.host_organization_name | Springer Nature |
| best_oa_location.source.host_organization_lineage | https://openalex.org/P4310319965 |
| best_oa_location.source.host_organization_lineage_names | Springer Nature |
| best_oa_location.license | cc-by |
| best_oa_location.pdf_url | |
| best_oa_location.version | publishedVersion |
| best_oa_location.raw_type | journal-article |
| best_oa_location.license_id | https://openalex.org/licenses/cc-by |
| best_oa_location.is_accepted | True |
| best_oa_location.is_published | True |
| best_oa_location.raw_source_name | ISME Communications |
| best_oa_location.landing_page_url | https://doi.org/10.1093/ismeco/ycae115 |
| primary_location.id | doi:10.1093/ismeco/ycae115 |
| primary_location.is_oa | True |
| primary_location.source.id | https://openalex.org/S4210240812 |
| primary_location.source.issn | 2730-6151 |
| primary_location.source.type | journal |
| primary_location.source.is_oa | True |
| primary_location.source.issn_l | 2730-6151 |
| primary_location.source.is_core | True |
| primary_location.source.is_in_doaj | True |
| primary_location.source.display_name | ISME Communications |
| primary_location.source.host_organization | https://openalex.org/P4310319965 |
| primary_location.source.host_organization_name | Springer Nature |
| primary_location.source.host_organization_lineage | https://openalex.org/P4310319965 |
| primary_location.source.host_organization_lineage_names | Springer Nature |
| primary_location.license | cc-by |
| primary_location.pdf_url | |
| primary_location.version | publishedVersion |
| primary_location.raw_type | journal-article |
| primary_location.license_id | https://openalex.org/licenses/cc-by |
| primary_location.is_accepted | True |
| primary_location.is_published | True |
| primary_location.raw_source_name | ISME Communications |
| primary_location.landing_page_url | https://doi.org/10.1093/ismeco/ycae115 |
| publication_date | 2024-01-01 |
| publication_year | 2024 |
| referenced_works | https://openalex.org/W2024471867, https://openalex.org/W2295190630, https://openalex.org/W2415100634, https://openalex.org/W2808974651, https://openalex.org/W2904248259, https://openalex.org/W3093980439, https://openalex.org/W3125263295, https://openalex.org/W4323050312, https://openalex.org/W1952026831, https://openalex.org/W3165153330, https://openalex.org/W3158094233, https://openalex.org/W2979186211, https://openalex.org/W3083397119, https://openalex.org/W2024169535, https://openalex.org/W2126295176, https://openalex.org/W6630071721, https://openalex.org/W2054385769, https://openalex.org/W2794904294, https://openalex.org/W4310773285, https://openalex.org/W2955065315, https://openalex.org/W3178755055 |
| referenced_works_count | 21 |
| abstract_inverted_index.= | 160, 164 |
| abstract_inverted_index.a | 64, 86 |
| abstract_inverted_index.r | 163 |
| abstract_inverted_index.By | 191 |
| abstract_inverted_index.To | 1 |
| abstract_inverted_index.at | 17, 82 |
| abstract_inverted_index.in | 187, 207 |
| abstract_inverted_index.it | 47 |
| abstract_inverted_index.of | 12, 105, 112, 131, 140, 144, 184 |
| abstract_inverted_index.to | 197 |
| abstract_inverted_index.we | 71, 193 |
| abstract_inverted_index.16S | 113, 136 |
| abstract_inverted_index.Our | 166 |
| abstract_inverted_index.and | 5, 15, 95, 128, 146 |
| abstract_inverted_index.but | 29 |
| abstract_inverted_index.for | 66, 203 |
| abstract_inverted_index.not | 56 |
| abstract_inverted_index.the | 90, 96, 106 |
| abstract_inverted_index.This | 102 |
| abstract_inverted_index.cell | 27, 129, 142, 182 |
| abstract_inverted_index.copy | 110, 137 |
| abstract_inverted_index.data | 151 |
| abstract_inverted_index.flow | 22, 149 |
| abstract_inverted_index.from | 78, 152 |
| abstract_inverted_index.gene | 109, 201 |
| abstract_inverted_index.high | 18 |
| abstract_inverted_index.into | 75 |
| abstract_inverted_index.mock | 126 |
| abstract_inverted_index.more | 42 |
| abstract_inverted_index.only | 30, 49 |
| abstract_inverted_index.rRNA | 114 |
| abstract_inverted_index.seek | 10 |
| abstract_inverted_index.show | 168 |
| abstract_inverted_index.taxa | 206 |
| abstract_inverted_index.that | 169 |
| abstract_inverted_index.this | 170, 195 |
| abstract_inverted_index.with | 34, 119, 125, 133, 148, 175 |
| abstract_inverted_index.1.01; | 161 |
| abstract_inverted_index.46-km | 83 |
| abstract_inverted_index.Gyre. | 101 |
| abstract_inverted_index.Here, | 70 |
| abstract_inverted_index.North | 98 |
| abstract_inverted_index.While | 38 |
| abstract_inverted_index.along | 85 |
| abstract_inverted_index.broad | 32 |
| abstract_inverted_index.field | 189 |
| abstract_inverted_index.known | 134 |
| abstract_inverted_index.offer | 63 |
| abstract_inverted_index.other | 204 |
| abstract_inverted_index.state | 4 |
| abstract_inverted_index.these | 208 |
| abstract_inverted_index.(slope | 159 |
| abstract_inverted_index.System | 94 |
| abstract_inverted_index.copies | 202 |
| abstract_inverted_index.counts | 183 |
| abstract_inverted_index.cruise | 87 |
| abstract_inverted_index.expect | 194 |
| abstract_inverted_index.groups | 33 |
| abstract_inverted_index.marine | 7, 185 |
| abstract_inverted_index.nearby | 153 |
| abstract_inverted_index.nearly | 156 |
| abstract_inverted_index.spiked | 72 |
| abstract_inverted_index.within | 45, 54 |
| abstract_inverted_index.Current | 93 |
| abstract_inverted_index.Pacific | 99 |
| abstract_inverted_index.complex | 188 |
| abstract_inverted_index.enabled | 103 |
| abstract_inverted_index.genomic | 61, 73, 135, 176 |
| abstract_inverted_index.optical | 36 |
| abstract_inverted_index.results | 158 |
| abstract_inverted_index.samples | 77 |
| abstract_inverted_index.surface | 79 |
| abstract_inverted_index.yielded | 155 |
| abstract_inverted_index.0.9942). | 165 |
| abstract_inverted_index.Abstract | 0 |
| abstract_inverted_index.Internal | 60 |
| abstract_inverted_index.absolute | 57, 67, 107, 181 |
| abstract_inverted_index.amplicon | 39, 115, 171 |
| abstract_inverted_index.approach | 196 |
| abstract_inverted_index.changes. | 59 |
| abstract_inverted_index.combined | 174 |
| abstract_inverted_index.discerns | 31 |
| abstract_inverted_index.distinct | 35 |
| abstract_inverted_index.estimate | 199 |
| abstract_inverted_index.findings | 167 |
| abstract_inverted_index.gathered | 81 |
| abstract_inverted_index.internal | 177 |
| abstract_inverted_index.measures | 180 |
| abstract_inverted_index.numbers. | 138 |
| abstract_inverted_index.plankton | 76 |
| abstract_inverted_index.primers, | 122 |
| abstract_inverted_index.protocol | 173 |
| abstract_inverted_index.provides | 50 |
| abstract_inverted_index.relative | 51 |
| abstract_inverted_index.resolves | 41 |
| abstract_inverted_index.samples, | 55 |
| abstract_inverted_index.samples. | 190, 209 |
| abstract_inverted_index.sequence | 116 |
| abstract_inverted_index.solution | 65 |
| abstract_inverted_index.southern | 91 |
| abstract_inverted_index.spanning | 89 |
| abstract_inverted_index.transect | 88 |
| abstract_inverted_index.variants | 117 |
| abstract_inverted_index.515Y-926R | 120 |
| abstract_inverted_index.abundance | 14, 28, 58 |
| abstract_inverted_index.amplified | 205 |
| abstract_inverted_index.cytometry | 23, 150 |
| abstract_inverted_index.diversity | 16, 44 |
| abstract_inverted_index.ecosystem | 3 |
| abstract_inverted_index.estimates | 25 |
| abstract_inverted_index.function, | 6 |
| abstract_inverted_index.identical | 157 |
| abstract_inverted_index.intervals | 84 |
| abstract_inverted_index.locations | 154 |
| abstract_inverted_index.measures. | 69 |
| abstract_inverted_index.microbial | 8, 26 |
| abstract_inverted_index.seawater, | 80 |
| abstract_inverted_index.standards | 62, 74, 178 |
| abstract_inverted_index.taxonomic | 19 |
| abstract_inverted_index.typically | 48 |
| abstract_inverted_index.universal | 121 |
| abstract_inverted_index.validated | 124 |
| abstract_inverted_index.(amplified | 118 |
| abstract_inverted_index.California | 92 |
| abstract_inverted_index.Comparison | 139 |
| abstract_inverted_index.abundances | 53, 111, 130, 143 |
| abstract_inverted_index.accurately | 24, 179 |
| abstract_inverted_index.ecologists | 9 |
| abstract_inverted_index.evaluation | 104 |
| abstract_inverted_index.extension, | 192 |
| abstract_inverted_index.organismal | 13, 52 |
| abstract_inverted_index.reasonably | 198 |
| abstract_inverted_index.sequencing | 40, 172 |
| abstract_inverted_index.understand | 2 |
| abstract_inverted_index.volumetric | 108, 200 |
| abstract_inverted_index.Pearson’s | 162 |
| abstract_inverted_index.Subtropical | 100 |
| abstract_inverted_index.properties. | 37 |
| abstract_inverted_index.resolution. | 20 |
| abstract_inverted_index.Conventional | 21 |
| abstract_inverted_index.communities) | 127 |
| abstract_inverted_index.measurements | 11 |
| abstract_inverted_index.microbiomes, | 46 |
| abstract_inverted_index.oligotrophic | 97 |
| abstract_inverted_index.Synechococcus | 147 |
| abstract_inverted_index.comprehensive | 43 |
| abstract_inverted_index.amplicon-based | 68 |
| abstract_inverted_index.quantitatively | 123 |
| abstract_inverted_index.Prochlorococcus | 145 |
| abstract_inverted_index.amplicon-derived | 141 |
| abstract_inverted_index.picocyanobacteria | 132, 186 |
| cited_by_percentile_year.max | 99 |
| cited_by_percentile_year.min | 90 |
| countries_distinct_count | 2 |
| institutions_distinct_count | 8 |
| sustainable_development_goals[0].id | https://metadata.un.org/sdg/14 |
| sustainable_development_goals[0].score | 0.8899999856948853 |
| sustainable_development_goals[0].display_name | Life below water |
| citation_normalized_percentile.value | 0.96936929 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | True |