Amplification of Telomeric Arrays via Rolling-circle Mechanism Article Swipe
Jozef Nosek
,
Adriana Ryčovská
,
Alexander M. Makhov
,
Jack D. Griffith
,
Ľubomír Tomáška
·
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.17615/k88m-6a49
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.17615/k88m-6a49
Alternative (telomerase-independent) lengthening of telomeres mediated through homologous recombination is often accompanied by a generation of extrachromosomal telomeric circles (t-circles), whose role in direct promotion of recombinational telomere elongation has been recently demonstrated. Here we present evidence that t-circles in a natural telomerase-deficient system of mitochondria of the yeast Candida parapsilosis replicate independently of the linear chromosome via a rolling-circle mechanism. This is supported by an observation of (i) single-stranded DNA consisting of concatameric arrays of telomeric sequence, (ii) lasso-shaped molecules representing rolling-circle intermediates, and (iii) preferential incorporation of deoxyribonucleotides into telomeric fragments and t-circles. Analysis of naturally occurring variant t-circles revealed conserved motifs with potential function in driving the rolling-circle replication. These data indicate that extrachromosomal t-circles observed in a wide variety of organisms, including yeasts, plants, Xenopus laevis, and certain human cell lines, may represent independent replicons generating telomeric sequences and, thus, actively participating in telomere dynamics. Moreover, because of the promiscuous occurrence of t-circles across phyla, the results from yeast mitochondria have implications related to the primordial system of telomere maintenance, providing a paradigm for evolution of telomeres in nuclei of early eukaryotes.
Related Topics
Concepts
Rolling circle replication
Mechanism (biology)
Materials science
Computer science
Biology
Genetics
Physics
DNA
DNA replication
Quantum mechanics
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https://doi.org/10.17615/k88m-6a49Digital Object Identifier
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Amplification of Telomeric Arrays via Rolling-circle MechanismWork title
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articleOpenAlex work type
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enPrimary language
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2021Year of publication
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2021-07-03Full publication date if available
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Jozef Nosek, Adriana Ryčovská, Alexander M. Makhov, Jack D. Griffith, Ľubomír TomáškaList of authors in order
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https://doi.org/10.17615/k88m-6a49Publisher landing page
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YesWhether a free full text is available
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greenOpen access status per OpenAlex
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https://doi.org/10.17615/k88m-6a49Direct OA link when available
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Rolling circle replication, Mechanism (biology), Materials science, Computer science, Biology, Genetics, Physics, DNA, DNA replication, Quantum mechanicsTop concepts (fields/topics) attached by OpenAlex
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0Total citation count in OpenAlex
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| citation_normalized_percentile.value | 0.12265611 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |