Journal of Immunological Methods • Vol 530
An easy assay to detect autoantibodies neutralizing cytokines in subjects with critical infections
May 2024 • Nicola Donadel, Alessandra Tesser, Erica Valencic, Eleonora De Martino, Valentina Boz, Alessia Pin, Francesca Zorat, Gabriele Pozzato, Alberto Tommas…
Autoantibodies against type I interferon (IFN) are associated with a worse outcome in COVID-19. The measurement of cytokine-neutralizing autoantibodies has been limited, hindering understanding of their role in clinical practice. We showed that an easy and reliable assay can be reproduced and validated to measure the neutralizing potency of autoantibodies directed to type I or type II IFN. Identifying of anti-cytokine autoantibodies might reflect on early treatments for subsequent infections, such as with antivira…