An easy method for preparation of Cre-loxP regulated fluorescent adenoviral expression vectors and its application for direct reprogramming into hepatocytes Article Swipe
Chitose Kurihara
,
Koji Nakade
,
Jianzhi Pan
,
Jing Huang
,
Bohdan Wasylyk
,
Yuichi Obata
·
YOU?
·
· 2016
· Open Access
·
· DOI: https://doi.org/10.1016/j.btre.2016.10.003
YOU?
·
· 2016
· Open Access
·
· DOI: https://doi.org/10.1016/j.btre.2016.10.003
The recombinant adenoviral gene expression system is a powerful tool for gene delivery. However, it is difficult to obtain high titers of infectious virus, principally due to the toxicity of the expressed gene which affects on virus replication in the host HEK293 cells. To avoid these problems, we generated a Cre-loxP-regulated fluorescent universal vector (termed pAxCALRL). This vector produces recombinant adenoviruses that express the red fluorescent protein (RFP) instead of the inserted gene during proliferation, which limits toxicity and can be used to monitor viral replication. Expression of the gene of interest is induced by co-infection with an adenovirus that expresses Cre-recombinase (AxCANCre). Recombinant adenovirus produced by this system that express Hnf4α and Foxa2 were used to reprogram mouse embryo fibroblast (MEF) into induced-hepatocyte-like cells (iHep) following several rounds of infection, demonstrating the efficacy of this new system.
Related Topics
Concepts
Viral vector
Biology
Green fluorescent protein
Recombinant DNA
Cre recombinase
HEK 293 cells
Gene delivery
Molecular biology
Reprogramming
Virology
Transfection
Gene expression
Gene
Cell biology
Transgene
Genetics
Genetically modified mouse
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1016/j.btre.2016.10.003
- OA Status
- gold
- Cited By
- 3
- References
- 24
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W2531455411
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W2531455411Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1016/j.btre.2016.10.003Digital Object Identifier
- Title
-
An easy method for preparation of Cre-loxP regulated fluorescent adenoviral expression vectors and its application for direct reprogramming into hepatocytesWork title
- Type
-
articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2016Year of publication
- Publication date
-
2016-10-10Full publication date if available
- Authors
-
Chitose Kurihara, Koji Nakade, Jianzhi Pan, Jing Huang, Bohdan Wasylyk, Yuichi ObataList of authors in order
- Landing page
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https://doi.org/10.1016/j.btre.2016.10.003Publisher landing page
- Open access
-
YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
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https://doi.org/10.1016/j.btre.2016.10.003Direct OA link when available
- Concepts
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Viral vector, Biology, Green fluorescent protein, Recombinant DNA, Cre recombinase, HEK 293 cells, Gene delivery, Molecular biology, Reprogramming, Virology, Transfection, Gene expression, Gene, Cell biology, Transgene, Genetics, Genetically modified mouseTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
3Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 1, 2022: 1, 2020: 1Per-year citation counts (last 5 years)
- References (count)
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24Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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