bioRxiv (Cold Spring Harbor Laboratory)
An Efficient Method to Quantify Structural Distributions in Heterogeneous cryo-EM Datasets
May 2021 • Hanlin Gu, Wei Wang, Ilona Christy Unarta, Wenqi Zeng, Fu Kit Sheong, Peter Pak‐Hang Cheung, Song Liu, Yuan Yao, Xuhui Huang
Abstract Cryogenic Electron Microscopy (cryo-EM) preserves the ensemble of protein conformations in solution and thus provide a promising way to characterize conformational changes underlying protein functions. However, it remains challenging for existing software to elucidate distributions of multiple conformations from a heterogeneous cryo-EM dataset. We developed a new algorithm: Linear Combinations of Template Conformations (LCTC) to obtain distributions of multiple conformations from cryo-EM datasets. LCTC as…