An NS5A single optimized method to determine genotype, subtype and resistance profiles of Hepatitis C strains Article Swipe
Élisabeth André-Garnier
,
Bernard Besse
,
Audrey Rodallec
,
Olivier Ribeyrol
,
Virginie Ferré
,
C. Luco
,
L Le Guen
,
Nathalie Bourgeois
,
Jérôme Gournay
,
Eric Billaud
,
F. Raffi
,
Marianne Coste‐Burel
,
Berthe‐Marie Imbert‐Marcille
·
YOU?
·
· 2017
· Open Access
·
· DOI: https://doi.org/10.1371/journal.pone.0179562
YOU?
·
· 2017
· Open Access
·
· DOI: https://doi.org/10.1371/journal.pone.0179562
The objective was to develop a method of HCV genome sequencing that allowed simultaneous genotyping and NS5A inhibitor resistance profiling. In order to validate the use of a unique RT-PCR for genotypes 1-5, 142 plasma samples from patients infected with HCV were analysed. The NS4B-NS5A partial region was successfully amplified and sequenced in all samples. In parallel, partial NS3 sequences were analyzed obtained for genotyping. Phylogenetic analysis showed concordance of genotypes and subtypes with a bootstrap >95% for each type cluster. NS5A resistance mutations were analyzed using the Geno2pheno [hcv] v0.92 tool and compared to the list of known Resistant Associated Substitutions recently published. In conclusion, this tool allows determination of HCV genotypes, subtypes and identification of NS5A resistance mutations. This single method can be used to detect pre-existing resistance mutations in NS5A before treatment and to check the emergence of resistant viruses while undergoing treatment in major HCV genotypes (G1-5) in the EU and the US.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1371/journal.pone.0179562
- https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0179562&type=printable
- OA Status
- gold
- Cited By
- 20
- References
- 26
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W2737244902
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W2737244902Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1371/journal.pone.0179562Digital Object Identifier
- Title
-
An NS5A single optimized method to determine genotype, subtype and resistance profiles of Hepatitis C strainsWork title
- Type
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articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2017Year of publication
- Publication date
-
2017-07-20Full publication date if available
- Authors
-
Élisabeth André-Garnier, Bernard Besse, Audrey Rodallec, Olivier Ribeyrol, Virginie Ferré, C. Luco, L Le Guen, Nathalie Bourgeois, Jérôme Gournay, Eric Billaud, F. Raffi, Marianne Coste‐Burel, Berthe‐Marie Imbert‐MarcilleList of authors in order
- Landing page
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https://doi.org/10.1371/journal.pone.0179562Publisher landing page
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https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0179562&type=printableDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
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https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0179562&type=printableDirect OA link when available
- Concepts
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NS5A, Genotyping, Genotype, Concordance, Biology, Genetics, Phylogenetic tree, Virology, Hepacivirus, GeneTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
20Total citation count in OpenAlex
- Citations by year (recent)
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2023: 3, 2022: 2, 2021: 4, 2020: 5, 2019: 4Per-year citation counts (last 5 years)
- References (count)
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26Number of works referenced by this work
- Related works (count)
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10Other works algorithmically related by OpenAlex
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