Analysis of combinatorial chemokine receptor expression dynamics using multi-receptor reporter mice Article Swipe

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Laura Medina‐Ruiz , Robin Bartolini , Douglas P. Dyer , Francesca Vidler , Catherine E. Hughes , Fabian Schuette , Samantha Love , Jun Fu , A. Francis Stewart , Gerard J. Graham ·

YOU? · · 2021 · Open Access · · DOI: https://doi.org/10.1101/2021.08.11.455927

Summary Inflammatory chemokines and their receptors are central to the development of inflammatory/immune pathologies. The apparent complexity of this system, coupled with lack of appropriate in vivo models, has limited our understanding of how chemokines orchestrate inflammatory responses and has hampered attempts at targeting this system in inflammatory disease. Novel approaches are therefore needed to provide crucial biological, and therapeutic, insights into the chemokine-chemokine receptor family. Here, we report the generation of transgenic multi-chemokine receptor reporter mice in which spectrally-distinct fluorescent reporters mark expression of CCRs 1, 2, 3 and 5, key receptors for myeloid cell recruitment in inflammation. Analysis of these animals has allowed us to define, for the first time, individual and combinatorial receptor expression patterns on myeloid cells in resting and inflamed conditions. Our results demonstrate that chemokine receptor expression is highly specific, and more selective than previously anticipated.

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CCR1 Chemokine receptor Chemokine Chemokine receptor CCR5 CCL21 CCR10 Biology C-C chemokine receptor type 6 Cell biology CCL13 CC chemokine receptors Receptor CXCL2 CX3CL1 Receptor expression Immunology CCR2 Inflammation Genetics

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Type: preprint
Language: en
Landing Page: https://doi.org/10.1101/2021.08.11.455927
PDF: https://www.biorxiv.org/content/biorxiv/early/2021/08/12/2021.08.11.455927.full.pdf
OA Status: green
Cited By: 2
References: 41
Related Works: 10
OpenAlex ID: https://openalex.org/W3191975650

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DOI: https://doi.org/10.1101/2021.08.11.455927

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Title: Analysis of combinatorial chemokine receptor expression dynamics using multi-receptor reporter mice

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Type: preprint

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Language: en

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Publication year: 2021

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Publication date: 2021-08-12

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Authors: Laura Medina‐Ruiz, Robin Bartolini, Douglas P. Dyer, Francesca Vidler, Catherine E. Hughes, Fabian Schuette, Samantha Love, Jun Fu, A. Francis Stewart, Gerard J. Graham

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Landing page: https://doi.org/10.1101/2021.08.11.455927

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PDF URL: https://www.biorxiv.org/content/biorxiv/early/2021/08/12/2021.08.11.455927.full.pdf

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OA URL: https://www.biorxiv.org/content/biorxiv/early/2021/08/12/2021.08.11.455927.full.pdf

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Concepts: CCR1, Chemokine receptor, Chemokine, Chemokine receptor CCR5, CCL21, CCR10, Biology, C-C chemokine receptor type 6, Cell biology, CCL13, CC chemokine receptors, Receptor, CXCL2, CX3CL1, Receptor expression, Immunology, CCR2, Inflammation, Genetics

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Cited by: 2

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Citations by year (recent): 2024: 1, 2022: 1

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References (count): 41

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Related works (count): 10

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