Anchoring of FRET Sensors—A Requirement for Spatiotemporal Resolution Article Swipe
Elena Ivanova
,
Ricardo Figueroa
,
Tom Gatsinzi
,
Einar Hallberg
,
Kerstin Iverfeldt
·
YOU?
·
· 2016
· Open Access
·
· DOI: https://doi.org/10.3390/s16050703
YOU?
·
· 2016
· Open Access
·
· DOI: https://doi.org/10.3390/s16050703
FRET biosensors have become a routine tool for investigating mechanisms and components of cell signaling. Strategies for improving them for particular applications are continuously sought. One important aspect to consider when designing FRET probes is the dynamic distribution and propagation of signals within living cells. We have addressed this issue by directly comparing an anchored (taFS) to a non-anchored (naFS) cleavable FRET sensor. We chose a microtubule-associated protein tau as an anchor, as microtubules are abundant throughout the cytosol of cells. We show that tau-anchored FRET sensors are concentrated at the cytoskeleton and enriched in the neurite-like processes of cells, providing high intensity of the total signal. In addition, anchoring limits the diffusion of the sensor, enabling spatiotemporally resolved monitoring of subcellular variations in enzyme activity. Thus, anchoring is an important aspect to consider when designing FRET sensors for deeper understanding of cell signaling.
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3390/s16050703
- https://www.mdpi.com/1424-8220/16/5/703/pdf?version=1463553181
- OA Status
- gold
- Cited By
- 3
- References
- 18
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W2350529470
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https://openalex.org/W2350529470Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.3390/s16050703Digital Object Identifier
- Title
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Anchoring of FRET Sensors—A Requirement for Spatiotemporal ResolutionWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2016Year of publication
- Publication date
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2016-05-16Full publication date if available
- Authors
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Elena Ivanova, Ricardo Figueroa, Tom Gatsinzi, Einar Hallberg, Kerstin IverfeldtList of authors in order
- Landing page
-
https://doi.org/10.3390/s16050703Publisher landing page
- PDF URL
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https://www.mdpi.com/1424-8220/16/5/703/pdf?version=1463553181Direct link to full text PDF
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YesWhether a free full text is available
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goldOpen access status per OpenAlex
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https://www.mdpi.com/1424-8220/16/5/703/pdf?version=1463553181Direct OA link when available
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Förster resonance energy transfer, Anchoring, Cytoskeleton, Biosensor, Microtubule, Nanotechnology, Biophysics, Neurite, Cytosol, Cell biology, Chemistry, Biology, Cell, Materials science, Physics, Enzyme, Fluorescence, Engineering, Biochemistry, Structural engineering, Quantum mechanics, In vitroTop concepts (fields/topics) attached by OpenAlex
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3Total citation count in OpenAlex
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2022: 1, 2018: 1, 2017: 1Per-year citation counts (last 5 years)
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18Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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