Assessment of an In-House Enzyme-Linked Immunosorbent Assay and IgG Avidity Test Based on SAG1 and GRA7 Proteins for Discriminating between Acute and Chronic Toxoplasmosis in Humans Article Swipe

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Avidity Toxoplasma gondii Toxoplasmosis Biology Antigen Virology Antibody Immunoglobulin M Immunoglobulin G Immunology

Aref Teimouri , Mohammad Javad Abbaszadeh Afshar , Sina Mohtasebi , Sanaz Jafarpour Azami , Rasoul Alimi , Hossein Keshavarz ·

YOU? · · 2021 · Open Access · · DOI: https://doi.org/10.1128/jcm.00416-21 · OA: W3165549050

To improve serodiagnostic methods for diagnosis of acute from chronic toxoplasmosis, an economical in-house enzyme-linked immunosorbent assay (ELISA) for measuring Toxoplasma -specific IgG, IgM, and IgG avidity has been developed and assessed based on use of various Toxoplasma gondii antigens, including SAG1, GRA7, and a combination of SAG1 and GRA7 (SAG1+GRA7), as well as Toxoplasma lysate antigens (TLAs). Performances of in-house IgM, IgG, and IgG avidity assays were compared to those of ELISA commercial kits and VIDAS Toxo IgG avidity.