Saudi Journal of Biological Sciences
Assessment of the R2R3 MYB gene expression profile during tomato fruit development using in silico analysis, quantitative and semi-quantitative RT-PCR
February 2022 • Abdallah A. Hassanin, Ahmed S. Eldomiaty, Javed Ahmed Ujjan, Amina A. M. Al-Mushhin, Ayshah Aysh ALrashidi, Ahmed M. Saad, Haifa A. S. Alhaithloul, M…
Assessment of gene expression is an essential and sensitive indicator of the biological activity and function of a certain gene. The expression profile of R2R3 MYB gene (Solyc02g067760) was investigated at ten development stages of tomato fruits (Lycopersicon esculentum) of Micro-Tom cultivar using three different approaches; quantitative RT-PCR, semi-quantitative RT-PCR and the promising Tomato Expression Atlas (TEA) bioinformatics tool. RNA was extracted from fruit tissues and used for synthesizing cDNA. Quantit…