Automated Quantification of Trophectoderm Morphology in Human Blastocysts via Instance Segmentation Article Swipe

PDF

Hang Liu , Shanshan Wang , Chen Sun , Guanqiao Shan , Wen‐Yuan Chen , Haocong Song , Changsheng Dai , Lei Chen , Zhuoran Zhang , Xingjian Liu , Haixiang Sun , Yu Sun ·

YOU? · · 2025 · Open Access · · DOI: https://doi.org/10.36227/techrxiv.175978790.03154264/v1

Segmenting individual trophectoderm (TE) cells is essential for developing quantitative metrics to assess the developmental potential of human blastocyst. The elongated shape and circular arrangement of TE cells lead to continuously varying orientations across the image, posing challenges for existing cell instance segmentation methods that assume uniformly oriented cells. As a result, most methods segment the TE as a single region, and the development of quantitative, cell-level metrics predictive of live birth potential remains unexplored. In this work, we propose an instance segmentation model that represents elongate, circularly arranged TE cells using elliptical distance maps, with which superior performance in both segmentation accuracy and metric extraction was achieved compared to state-of-the-art methods. The extracted metrics, including TE cell number, the mean and standard deviation of cell length, width, area, and mean inter-cell distance, serve as effective predictors of a blastocyst’s live birth potential. When used to predict live birth, these metrics achieved a significantly higher area under the receiver operating characteristic curve (AUC = 0.693) than traditional TE morphological grades (AUC = 0.585). Source codes are made available at https://github.com/robotVisionHang/TESeg.

Related Topics

Automated Guided Vehicle

Human

Effects Of Climate Change On Human Health

Human Sexual Activity

The Human Centipede (First Sequence)

Human Right To Water And Sanitation

Human Sexuality

Human Papillomavirus Infection

Human Evolution

Human–Robot Interaction

The Human League

Detroit: Become Human

Human Anus

Race (Human Categorization)

Urban Morphology

Human History

Human Trafficking

List Of Bones Of The Human Skeleton

No Longer Human

Concepts

No concepts available.

Metadata

Type: preprint
Language: en
Landing Page: https://doi.org/10.36227/techrxiv.175978790.03154264/v1
PDF: https://www.techrxiv.org/doi/pdf/10.36227/techrxiv.175978790.03154264
OA Status: gold
OpenAlex ID: https://openalex.org/W4414860323

All OpenAlex metadata

Raw OpenAlex JSON

OpenAlex ID: https://openalex.org/W4414860323

Canonical identifier for this work in OpenAlex
DOI: https://doi.org/10.36227/techrxiv.175978790.03154264/v1

Digital Object Identifier
Title: Automated Quantification of Trophectoderm Morphology in Human Blastocysts via Instance Segmentation

Work title
Type: preprint

OpenAlex work type
Language: en

Primary language
Publication year: 2025

Year of publication
Publication date: 2025-10-06

Full publication date if available
Authors: Hang Liu, Shanshan Wang, Chen Sun, Guanqiao Shan, Wen‐Yuan Chen, Haocong Song, Changsheng Dai, Lei Chen, Zhuoran Zhang, Xingjian Liu, Haixiang Sun, Yu Sun

List of authors in order
Landing page: https://doi.org/10.36227/techrxiv.175978790.03154264/v1

Publisher landing page
PDF URL: https://www.techrxiv.org/doi/pdf/10.36227/techrxiv.175978790.03154264

Direct link to full text PDF
Open access: Yes

Whether a free full text is available
OA status: gold

Open access status per OpenAlex
OA URL: https://www.techrxiv.org/doi/pdf/10.36227/techrxiv.175978790.03154264

Direct OA link when available
Cited by: 0

Total citation count in OpenAlex

Full payload

id	https://openalex.org/W4414860323
doi	https://doi.org/10.36227/techrxiv.175978790.03154264/v1
ids.doi	https://doi.org/10.36227/techrxiv.175978790.03154264/v1
ids.openalex	https://openalex.org/W4414860323
fwci	0.0
type	preprint
title	Automated Quantification of Trophectoderm Morphology in Human Blastocysts via Instance Segmentation
biblio.issue
biblio.volume
biblio.last_page
biblio.first_page
topics[0].id	https://openalex.org/T11978
topics[0].field.id	https://openalex.org/fields/22
topics[0].field.display_name	Engineering
topics[0].score	0.6284000277519226
topics[0].domain.id	https://openalex.org/domains/3
topics[0].domain.display_name	Physical Sciences
topics[0].subfield.id	https://openalex.org/subfields/2205
topics[0].subfield.display_name	Civil and Structural Engineering
topics[0].display_name	Soil Mechanics and Vehicle Dynamics
topics[1].id	https://openalex.org/T14009
topics[1].field.id	https://openalex.org/fields/11
topics[1].field.display_name	Agricultural and Biological Sciences
topics[1].score	0.5789999961853027
topics[1].domain.id	https://openalex.org/domains/1
topics[1].domain.display_name	Life Sciences
topics[1].subfield.id	https://openalex.org/subfields/1110
topics[1].subfield.display_name	Plant Science
topics[1].display_name	Ecology and Conservation Studies
is_xpac	False
apc_list
apc_paid
language	en
locations[0].id	doi:10.36227/techrxiv.175978790.03154264/v1
locations[0].is_oa	True
locations[0].source
locations[0].license	cc-by-nc-sa
locations[0].pdf_url	https://www.techrxiv.org/doi/pdf/10.36227/techrxiv.175978790.03154264
locations[0].version	acceptedVersion
locations[0].raw_type	posted-content
locations[0].license_id	https://openalex.org/licenses/cc-by-nc-sa
locations[0].is_accepted	True
locations[0].is_published	False
locations[0].raw_source_name
locations[0].landing_page_url	https://doi.org/10.36227/techrxiv.175978790.03154264/v1
indexed_in	crossref
authorships[0].author.id	https://openalex.org/A5100338895
authorships[0].author.orcid	https://orcid.org/0000-0001-7948-4236
authorships[0].author.display_name	Hang Liu
authorships[0].author_position	first
authorships[0].raw_author_name	Hang Liu
authorships[0].is_corresponding	False
authorships[1].author.id	https://openalex.org/A5100406282
authorships[1].author.orcid	https://orcid.org/0009-0004-1322-3032
authorships[1].author.display_name	Shanshan Wang
authorships[1].author_position	middle
authorships[1].raw_author_name	Shanshan Wang
authorships[1].is_corresponding	False
authorships[2].author.id	https://openalex.org/A5103004111
authorships[2].author.orcid	https://orcid.org/0009-0000-5776-7825
authorships[2].author.display_name	Chen Sun
authorships[2].author_position	middle
authorships[2].raw_author_name	Chen Sun
authorships[2].is_corresponding	False
authorships[3].author.id	https://openalex.org/A5066331381
authorships[3].author.orcid	https://orcid.org/0000-0002-2570-769X
authorships[3].author.display_name	Guanqiao Shan
authorships[3].author_position	middle
authorships[3].raw_author_name	Guanqiao Shan
authorships[3].is_corresponding	False
authorships[4].author.id	https://openalex.org/A5101827049
authorships[4].author.orcid	https://orcid.org/0000-0001-8652-7441
authorships[4].author.display_name	Wen‐Yuan Chen
authorships[4].author_position	middle
authorships[4].raw_author_name	Wenyuan Chen
authorships[4].is_corresponding	False
authorships[5].author.id	https://openalex.org/A5014097801
authorships[5].author.orcid	https://orcid.org/0009-0001-3888-6850
authorships[5].author.display_name	Haocong Song
authorships[5].author_position	middle
authorships[5].raw_author_name	Haocong Song
authorships[5].is_corresponding	False
authorships[6].author.id	https://openalex.org/A5037119790
authorships[6].author.orcid	https://orcid.org/0000-0002-8293-0097
authorships[6].author.display_name	Changsheng Dai
authorships[6].author_position	middle
authorships[6].raw_author_name	Changsheng Dai
authorships[6].is_corresponding	False
authorships[7].author.id	https://openalex.org/A5100333580
authorships[7].author.orcid	https://orcid.org/0000-0003-3989-9239
authorships[7].author.display_name	Lei Chen
authorships[7].author_position	middle
authorships[7].raw_author_name	Lei Chen
authorships[7].is_corresponding	False
authorships[8].author.id	https://openalex.org/A5100652271
authorships[8].author.orcid	https://orcid.org/0000-0002-8737-4210
authorships[8].author.display_name	Zhuoran Zhang
authorships[8].author_position	middle
authorships[8].raw_author_name	Zhuoran Zhang
authorships[8].is_corresponding	False
authorships[9].author.id	https://openalex.org/A5050119809
authorships[9].author.orcid
authorships[9].author.display_name	Xingjian Liu
authorships[9].author_position	middle
authorships[9].raw_author_name	Xingjian Liu
authorships[9].is_corresponding	False
authorships[10].author.id	https://openalex.org/A5100667322
authorships[10].author.orcid	https://orcid.org/0000-0002-1215-1792
authorships[10].author.display_name	Haixiang Sun
authorships[10].author_position	middle
authorships[10].raw_author_name	Haixiang Sun
authorships[10].is_corresponding	False
authorships[11].author.id	https://openalex.org/A5115595077
authorships[11].author.orcid	https://orcid.org/0000-0002-5364-2387
authorships[11].author.display_name	Yu Sun
authorships[11].author_position	last
authorships[11].raw_author_name	Yu Sun
authorships[11].is_corresponding	False
has_content.pdf	True
has_content.grobid_xml	False
is_paratext	False
open_access.is_oa	True
open_access.oa_url	https://www.techrxiv.org/doi/pdf/10.36227/techrxiv.175978790.03154264
open_access.oa_status	gold
open_access.any_repository_has_fulltext	False
created_date	2025-10-10T00:00:00
display_name	Automated Quantification of Trophectoderm Morphology in Human Blastocysts via Instance Segmentation
has_fulltext	False
is_retracted	False
updated_date	2025-11-06T03:46:38.306776
primary_topic.id	https://openalex.org/T11978
primary_topic.field.id	https://openalex.org/fields/22
primary_topic.field.display_name	Engineering
primary_topic.score	0.6284000277519226
primary_topic.domain.id	https://openalex.org/domains/3
primary_topic.domain.display_name	Physical Sciences
primary_topic.subfield.id	https://openalex.org/subfields/2205
primary_topic.subfield.display_name	Civil and Structural Engineering
primary_topic.display_name	Soil Mechanics and Vehicle Dynamics
cited_by_count	0
locations_count	1
best_oa_location.id	doi:10.36227/techrxiv.175978790.03154264/v1
best_oa_location.is_oa	True
best_oa_location.source
best_oa_location.license	cc-by-nc-sa
best_oa_location.pdf_url	https://www.techrxiv.org/doi/pdf/10.36227/techrxiv.175978790.03154264
best_oa_location.version	acceptedVersion
best_oa_location.raw_type	posted-content
best_oa_location.license_id	https://openalex.org/licenses/cc-by-nc-sa
best_oa_location.is_accepted	True
best_oa_location.is_published	False
best_oa_location.raw_source_name
best_oa_location.landing_page_url	https://doi.org/10.36227/techrxiv.175978790.03154264/v1
primary_location.id	doi:10.36227/techrxiv.175978790.03154264/v1
primary_location.is_oa	True
primary_location.source
primary_location.license	cc-by-nc-sa
primary_location.pdf_url	https://www.techrxiv.org/doi/pdf/10.36227/techrxiv.175978790.03154264
primary_location.version	acceptedVersion
primary_location.raw_type	posted-content
primary_location.license_id	https://openalex.org/licenses/cc-by-nc-sa
primary_location.is_accepted	True
primary_location.is_published	False
primary_location.raw_source_name
primary_location.landing_page_url	https://doi.org/10.36227/techrxiv.175978790.03154264/v1
publication_date	2025-10-06
publication_year	2025
referenced_works_count	0
abstract_inverted_index.=	163, 171
abstract_inverted_index.a	50, 58, 138, 152
abstract_inverted_index.As	49
abstract_inverted_index.In	75
abstract_inverted_index.TE	26, 56, 89, 116, 167
abstract_inverted_index.an	80
abstract_inverted_index.as	57, 134
abstract_inverted_index.at	178
abstract_inverted_index.in	99
abstract_inverted_index.is	5
abstract_inverted_index.of	16, 25, 64, 69, 124, 137
abstract_inverted_index.to	11, 29, 109, 145
abstract_inverted_index.we	78
abstract_inverted_index.The	19, 112
abstract_inverted_index.and	22, 61, 103, 121, 129
abstract_inverted_index.are	175
abstract_inverted_index.for	7, 38
abstract_inverted_index.the	13, 34, 55, 62, 119, 157
abstract_inverted_index.was	106
abstract_inverted_index.(AUC	162, 170
abstract_inverted_index.(TE)	3
abstract_inverted_index.When	143
abstract_inverted_index.area	155
abstract_inverted_index.both	100
abstract_inverted_index.cell	40, 117, 125
abstract_inverted_index.lead	28
abstract_inverted_index.live	70, 140, 147
abstract_inverted_index.made	176
abstract_inverted_index.mean	120, 130
abstract_inverted_index.most	52
abstract_inverted_index.than	165
abstract_inverted_index.that	44, 84
abstract_inverted_index.this	76
abstract_inverted_index.used	144
abstract_inverted_index.with	95
abstract_inverted_index.area,	128
abstract_inverted_index.birth	71, 141
abstract_inverted_index.cells	4, 27, 90
abstract_inverted_index.codes	174
abstract_inverted_index.curve	161
abstract_inverted_index.human	17
abstract_inverted_index.maps,	94
abstract_inverted_index.model	83
abstract_inverted_index.serve	133
abstract_inverted_index.shape	21
abstract_inverted_index.these	149
abstract_inverted_index.under	156
abstract_inverted_index.using	91
abstract_inverted_index.which	96
abstract_inverted_index.work,	77
abstract_inverted_index.0.693)	164
abstract_inverted_index.Source	173
abstract_inverted_index.across	33
abstract_inverted_index.assess	12
abstract_inverted_index.assume	45
abstract_inverted_index.birth,	148
abstract_inverted_index.cells.	48
abstract_inverted_index.grades	169
abstract_inverted_index.higher	154
abstract_inverted_index.image,	35
abstract_inverted_index.metric	104
abstract_inverted_index.posing	36
abstract_inverted_index.single	59
abstract_inverted_index.width,	127
abstract_inverted_index.0.585).	172
abstract_inverted_index.length,	126
abstract_inverted_index.methods	43, 53
abstract_inverted_index.metrics	10, 67, 150
abstract_inverted_index.number,	118
abstract_inverted_index.predict	146
abstract_inverted_index.propose	79
abstract_inverted_index.region,	60
abstract_inverted_index.remains	73
abstract_inverted_index.result,	51
abstract_inverted_index.segment	54
abstract_inverted_index.varying	31
abstract_inverted_index.accuracy	102
abstract_inverted_index.achieved	107, 151
abstract_inverted_index.arranged	88
abstract_inverted_index.circular	23
abstract_inverted_index.compared	108
abstract_inverted_index.distance	93
abstract_inverted_index.existing	39
abstract_inverted_index.instance	41, 81
abstract_inverted_index.methods.	111
abstract_inverted_index.metrics,	114
abstract_inverted_index.oriented	47
abstract_inverted_index.receiver	158
abstract_inverted_index.standard	122
abstract_inverted_index.superior	97
abstract_inverted_index.available	177
abstract_inverted_index.deviation	123
abstract_inverted_index.distance,	132
abstract_inverted_index.effective	135
abstract_inverted_index.elongate,	86
abstract_inverted_index.elongated	20
abstract_inverted_index.essential	6
abstract_inverted_index.extracted	113
abstract_inverted_index.including	115
abstract_inverted_index.operating	159
abstract_inverted_index.potential	15, 72
abstract_inverted_index.uniformly	46
abstract_inverted_index.Segmenting	0
abstract_inverted_index.cell-level	66
abstract_inverted_index.challenges	37
abstract_inverted_index.circularly	87
abstract_inverted_index.developing	8
abstract_inverted_index.elliptical	92
abstract_inverted_index.extraction	105
abstract_inverted_index.individual	1
abstract_inverted_index.inter-cell	131
abstract_inverted_index.potential.	142
abstract_inverted_index.predictive	68
abstract_inverted_index.predictors	136
abstract_inverted_index.represents	85
abstract_inverted_index.arrangement	24
abstract_inverted_index.blastocyst.	18
abstract_inverted_index.development	63
abstract_inverted_index.performance	98
abstract_inverted_index.traditional	166
abstract_inverted_index.unexplored.	74
abstract_inverted_index.continuously	30
abstract_inverted_index.orientations	32
abstract_inverted_index.quantitative	9
abstract_inverted_index.segmentation	42, 82, 101
abstract_inverted_index.developmental	14
abstract_inverted_index.morphological	168
abstract_inverted_index.quantitative,	65
abstract_inverted_index.significantly	153
abstract_inverted_index.trophectoderm	2
abstract_inverted_index.blastocyst’s	139
abstract_inverted_index.characteristic	160
abstract_inverted_index.state-of-the-art	110
abstract_inverted_index.https://github.com/robotVisionHang/TESeg.	179
cited_by_percentile_year
countries_distinct_count	0
institutions_distinct_count	12
citation_normalized_percentile.value	0.52422328
citation_normalized_percentile.is_in_top_1_percent	False
citation_normalized_percentile.is_in_top_10_percent	False