Automation and miniaturization of solid-phase extraction for high-throughput analysis of cyanotoxins Article Swipe
Thomas Melzer
,
Thomas Wichard
,
Robert Möller
,
Nico Ueberschaar
,
Georg Pohnert
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1016/j.chroma.2025.465828
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1016/j.chroma.2025.465828
We present a resource-efficient method for automated sample preparation, designated to facilitate high-throughput analysis of microcystins and nodularin-R. These cyanotoxins, commonly associated with harmful algal blooms, are monitored to ensure the safety of drinking water and recreational water bodies. The method utilizes a liquid handling platform to flexibly process between 1 and 96 samples within one hour for subsequent LC-MS analysis, requiring only 5 mL of sample for triplicate analysis. By miniaturizing solid-phase extraction into a 96-well format, the protocol reduces transfer volumes, and solvent consumption and has the potential to decrease the use of plastic materials. This approach is suitable for both high-throughput analyses and long-term monitoring studies, and it has been successfully applied to diverse water sources, including river, lake, pond, and HPLC-grade water. The method demonstrates excellent accuracy, with results meeting recommended guidelines. As proof of concept, it was applied to quantify cyanotoxins in two lake water samples and a laboratory culture of Microcystis aeruginosa.
Related Topics
Concepts
Chemistry
Miniaturization
Automation
Chromatography
Solid phase extraction
Throughput
Extraction (chemistry)
Solid-phase microextraction
Nanotechnology
Computer science
Gas chromatography–mass spectrometry
Engineering
Mass spectrometry
Telecommunications
Wireless
Materials science
Mechanical engineering
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1016/j.chroma.2025.465828
- OA Status
- hybrid
- Cited By
- 2
- References
- 61
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4408059905
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4408059905Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.1016/j.chroma.2025.465828Digital Object Identifier
- Title
-
Automation and miniaturization of solid-phase extraction for high-throughput analysis of cyanotoxinsWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2025Year of publication
- Publication date
-
2025-03-01Full publication date if available
- Authors
-
Thomas Melzer, Thomas Wichard, Robert Möller, Nico Ueberschaar, Georg PohnertList of authors in order
- Landing page
-
https://doi.org/10.1016/j.chroma.2025.465828Publisher landing page
- Open access
-
YesWhether a free full text is available
- OA status
-
hybridOpen access status per OpenAlex
- OA URL
-
https://doi.org/10.1016/j.chroma.2025.465828Direct OA link when available
- Concepts
-
Chemistry, Miniaturization, Automation, Chromatography, Solid phase extraction, Throughput, Extraction (chemistry), Solid-phase microextraction, Nanotechnology, Computer science, Gas chromatography–mass spectrometry, Engineering, Mass spectrometry, Telecommunications, Wireless, Materials science, Mechanical engineeringTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
2Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 2Per-year citation counts (last 5 years)
- References (count)
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61Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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