Avaliação in vitro de Membranas Poliméricas Carregadas com Vitamina D e Dexametasona na Engenharia Tecidual Óssea Article Swipe
Flávio Alessandro Serrão Gonçalves
,
D P Lopes
,
M. S. Moreira
,
Victor E. Arana‐Chaves
,
Luiz Henrique Catalani
·
YOU?
·
· 2018
· Open Access
·
· DOI: https://doi.org/10.17921/2447-8938.2017v19n5p80
YOU?
·
· 2018
· Open Access
·
· DOI: https://doi.org/10.17921/2447-8938.2017v19n5p80
Os objetivos desse estudo foram: desenvolver e caracterizar membranas poliméricas de ácido poli-L-láctico (PLLA) associado à dexametasona (DX) e vitamina D (VD) e avaliar a citotoxicidade e capacidade de osteoindução desses materiais na presença de células-tronco do ligamento periodontal (PDLSC). Foram confeccionadas membranas por meio de eletrofiação compostas por PLLA, PLLA/DX ou PLLA/VD, e as concentrações de DX e VD foram 1, 5 ou 10% em peso. A morfologia das malhas foi avaliada por microscopia eletrônica de varredura (MEV). As membranas foram armazenadas por 14 dias em meio de cultura e o meio condicionado coletado foi adicionado sobre cultura de PDLSC para análise da citotoxicidade por meio de ensaio de redução do MTT em 24 e 72h. Diferenciação óssea foi determinada por meio de ensaio de vermelho de alizarina após 21 dias de cultura das PDLSC sobre as membranas, em meio de diferenciação osteogênica. Observou-se que a eletrofiação produziu fibras homogêneas e sem gotas em todos materiais. Não houve diferenças estatísticas quanto à viabilidade celular, de forma que todas as membranas produzidas foram atóxicas às PDLSCs em 24 e 72h (88±10% e 95±10%). Os materiais com 1 e 5% de vitamina D e 1% de dexametasona promoveram maior diferenciação celular, com absorbância de 0,7 ± 0,1 e 0,8 ± 0,1 e 0,5± 0,1, respectivamente, e diferiram do controle de PLLA (0,28 ± 0,01). Desta forma, podemos concluir que as membranas desenvolvidas foram biocompatíveis em PDLSC e propiciaram aumento na osteodiferenciação quando 1% de dexametasona ou 1 e 5% de vitamina D foram associados ao PLLA.Palavras-chave: Regeneração Óssea. Células-Tronco. Engenharia Tecidual.
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Metadata
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All OpenAlex metadata
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https://openalex.org/W2800957274Canonical identifier for this work in OpenAlex
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https://doi.org/10.17921/2447-8938.2017v19n5p80Digital Object Identifier
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Avaliação in vitro de Membranas Poliméricas Carregadas com Vitamina D e Dexametasona na Engenharia Tecidual ÓsseaWork title
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articleOpenAlex work type
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ptPrimary language
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2018Year of publication
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2018-02-23Full publication date if available
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Flávio Alessandro Serrão Gonçalves, D P Lopes, M. S. Moreira, Victor E. Arana‐Chaves, Luiz Henrique CatalaniList of authors in order
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https://doi.org/10.17921/2447-8938.2017v19n5p80Publisher landing page
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YesWhether a free full text is available
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diamondOpen access status per OpenAlex
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https://doi.org/10.17921/2447-8938.2017v19n5p80Direct OA link when available
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Chemistry, Molecular biology, Physics, BiologyTop concepts (fields/topics) attached by OpenAlex
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0Total citation count in OpenAlex
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10Other works algorithmically related by OpenAlex
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