Awakening of the RuMP cycle for partial methylotrophy in the thermophile Parageobacillus thermoglucosidasius Article Swipe
Miguel Paredes-Barrada
,
Annemieke S. Mathissen
,
Roland A. van der Molen
,
Pablo J. Jiménez-Huesa
,
Maurizio Polano
,
Stefano Donati
,
Miriam Abele
,
Christina Ludwig
,
Richard van Kranenburg
,
Nico J. Claassens
·
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1101/2024.10.31.621308
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1101/2024.10.31.621308
Given sustainability and scalability concerns of using sugar feedstocks for microbial bioproduction of bulk chemicals, widening the feedstock range for microbial cell factories is of high interest. Methanol is a one-carbon alcohol that stands out as an alternative feedstock for the bioproduction of chemicals, as it is electron-rich, water-miscible and can be produced from several renewable resources. Bioconversion of methanol into products under thermophilic conditions (>50°C) could be highly advantageous for industrial biotechnology. Although progress is being made with natural, thermophilic methylotrophic microorganisms, they are not yet optimal for bioproduction and establishing alternative thermophilic methylotrophic bioproduction platforms can widen possibilities. Hence, we set out to implement synthetic methanol assimilation in the emerging thermophilic model organism Parageobacillus thermoglucosidasius. We engineered P. thermoglucosidasius to be strictly dependent for its growth on methanol assimilation via the core of the highly efficient ribulose monophosphate (RuMP) cycle, while co-assimilating ribose. Surprisingly, this did not require heterologous expression of RuMP enzymes. Instead, by laboratory evolution we awakened latent, native enzyme activities to form the core of the RuMP cycle. We obtained fast methylotrophic growth in which ∼17% of biomass was strictly obtained from methanol. This work lays the foundation for developing a versatile thermophilic bioproduction platform based on renewable methanol.
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Metadata
- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.1101/2024.10.31.621308
- https://www.biorxiv.org/content/biorxiv/early/2024/10/31/2024.10.31.621308.full.pdf
- OA Status
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- Cited By
- 1
- References
- 64
- Related Works
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- OpenAlex ID
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All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4403929718Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1101/2024.10.31.621308Digital Object Identifier
- Title
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Awakening of the RuMP cycle for partial methylotrophy in the thermophile Parageobacillus thermoglucosidasiusWork title
- Type
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preprintOpenAlex work type
- Language
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enPrimary language
- Publication year
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2024Year of publication
- Publication date
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2024-10-31Full publication date if available
- Authors
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Miguel Paredes-Barrada, Annemieke S. Mathissen, Roland A. van der Molen, Pablo J. Jiménez-Huesa, Maurizio Polano, Stefano Donati, Miriam Abele, Christina Ludwig, Richard van Kranenburg, Nico J. ClaassensList of authors in order
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https://doi.org/10.1101/2024.10.31.621308Publisher landing page
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https://www.biorxiv.org/content/biorxiv/early/2024/10/31/2024.10.31.621308.full.pdfDirect link to full text PDF
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YesWhether a free full text is available
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greenOpen access status per OpenAlex
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https://www.biorxiv.org/content/biorxiv/early/2024/10/31/2024.10.31.621308.full.pdfDirect OA link when available
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Rump, Thermophile, Computer science, Computational biology, Chemistry, Biology, Biochemistry, Anatomy, EnzymeTop concepts (fields/topics) attached by OpenAlex
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1Total citation count in OpenAlex
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2025: 1Per-year citation counts (last 5 years)
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64Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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