Bacterial flagella disrupt host cell membranes and interact with cytoskeletal components Article Swipe

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Eliza B. Wolfson , Johanna Elvidge , Amin Tahoun , Trudi Gillespie , Judith Mantell , Sean P. McAteer , Yannick Rossez , Edith Paxton , Fiona Lane , Darren J. Shaw , Andrew C. Gill , Jo Stevens , Paul Verkade , Ariel Blocker , Arvind Mahajan , David L. Gally ·

YOU? · · 2020 · Open Access · · DOI: https://doi.org/10.1101/2020.02.12.945204

Bacterial flagella have many established roles beyond swimming motility. Despite clear evidence of flagella-dependent adherence, the specificity of ligands and mechanisms of binding are still debated. In this study, the molecular basis of E. coli O157:H7 and S . Typhimurium flagella binding to epithelial cell cultures was investigated. Flagella interactions with host cell surfaces were intimate and crossed cellular boundaries as demarcated by actin and membrane labelling. SEM revealed flagella disappearing into cellular surfaces and TEM of S . Typhiumurium indicated host membrane deformation and disruption in proximity to flagella. Motor mutants of E. coli O157:H7 and S . Typhimurium caused reduced haemolysis compared to wild-type, indicating that membrane disruption was in part due to flagella rotation. Flagella from E. coli O157 (H7), EPEC O127 (H6), and S . Typhimurium (P1 & P2 flagella) were shown to bind to purified intracellular components of the actin cytoskeleton and directly increase in vitro actin polymerisation rates.

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Flagellum Cell biology Cytoskeleton Biology Actin Motility Intracellular Membrane Microbiology Cell Bacteria Biochemistry Genetics

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Type: preprint
Language: en
Landing Page: https://doi.org/10.1101/2020.02.12.945204
PDF: https://www.biorxiv.org/content/biorxiv/early/2020/02/13/2020.02.12.945204.full.pdf
OA Status: green
Cited By: 4
References: 75
Related Works: 10
OpenAlex ID: https://openalex.org/W3006498221

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DOI: https://doi.org/10.1101/2020.02.12.945204

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Title: Bacterial flagella disrupt host cell membranes and interact with cytoskeletal components

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Type: preprint

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Language: en

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Publication year: 2020

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Publication date: 2020-02-13

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Authors: Eliza B. Wolfson, Johanna Elvidge, Amin Tahoun, Trudi Gillespie, Judith Mantell, Sean P. McAteer, Yannick Rossez, Edith Paxton, Fiona Lane, Darren J. Shaw, Andrew C. Gill, Jo Stevens, Paul Verkade, Ariel Blocker, Arvind Mahajan, David L. Gally

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Landing page: https://doi.org/10.1101/2020.02.12.945204

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PDF URL: https://www.biorxiv.org/content/biorxiv/early/2020/02/13/2020.02.12.945204.full.pdf

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Concepts: Flagellum, Cell biology, Cytoskeleton, Biology, Actin, Motility, Intracellular, Membrane, Microbiology, Cell, Bacteria, Biochemistry, Genetics

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Cited by: 4

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abstract_inverted_index.components	142
abstract_inverted_index.demarcated	62
abstract_inverted_index.disruption	86, 110
abstract_inverted_index.epithelial	44
abstract_inverted_index.haemolysis	103
abstract_inverted_index.indicating	107
abstract_inverted_index.labelling.	67
abstract_inverted_index.mechanisms	21
abstract_inverted_index.wild-type,	106
abstract_inverted_index.Typhimurium	40, 100, 130
abstract_inverted_index.deformation	84
abstract_inverted_index.established	5
abstract_inverted_index.specificity	17
abstract_inverted_index.Typhiumurium	80
abstract_inverted_index.cytoskeleton	146
abstract_inverted_index.disappearing	71
abstract_inverted_index.interactions	50
abstract_inverted_index.intracellular	141
abstract_inverted_index.investigated.	48
abstract_inverted_index.polymerisation	153
abstract_inverted_index.flagella-dependent	14
cited_by_percentile_year.max	97
cited_by_percentile_year.min	96
corresponding_author_ids	https://openalex.org/A5000572566, https://openalex.org/A5112758594
countries_distinct_count	3
institutions_distinct_count	16
corresponding_institution_ids	https://openalex.org/I133960621, https://openalex.org/I98677209
sustainable_development_goals[0].id	https://metadata.un.org/sdg/14
sustainable_development_goals[0].score	0.7599999904632568
sustainable_development_goals[0].display_name	Life below water
citation_normalized_percentile.value	0.57922698
citation_normalized_percentile.is_in_top_1_percent	False
citation_normalized_percentile.is_in_top_10_percent	False