BASIC EXPERIMENT ON DNA TRACER FOR THE VISUALIZATION OF GROUND WATER DYNAMICS Article Swipe

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Yoshihisa AKAMATSU , Humiko IMAMURA , Ryohei Nakao , Masuji Goto ·

YOU? · · 2020 · Open Access · · DOI: https://doi.org/10.2208/jscejhe.76.2_i_1027

地下水の流動は「トレーサー試験」によって把握するのが一般的である．本研究ではDNAを利用した新たなトレーサーを開発することを目的として，DNAトレーサーの作製とその有効性に関して基礎的な実験を行った．水中などの環境中のDNA分析ではフィルタリングしたものからDNAを抽出して，リアルタイム定量PCR分析をかけるため，むき出しのプラスミドDNAはトレーサーとして利用できないことがわかった．しかし，DNAをリポソームに封入した場合にはフィルターでのトラップが容易となり，DNAが96時間程度分解されないことが明らかとなった．また，このリポソーム封入DNAを用いて，1mの珪砂入り管路での地下水浸透実験を実施したところ，塩分とほぼ同様の動態を示し，地下水動態のトレーサーとして利用可能であることが示された．

Related Topics

Dna

Visualization (Graphics)

Polymerase Chain Reaction

Reverse Transcriptase

Artificial Intelligence

Concepts

DNA Visualization DNA clamp Biophysics Chemistry Molecular biology Genetics Biology Computer science Polymerase chain reaction Gene Reverse transcriptase Artificial intelligence

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Type: article
Language: en
Landing Page: https://doi.org/10.2208/jscejhe.76.2_i_1027
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DOI: https://doi.org/10.2208/jscejhe.76.2_i_1027

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Title: BASIC EXPERIMENT ON DNA TRACER FOR THE VISUALIZATION OF GROUND WATER DYNAMICS

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Type: article

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Language: en

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Publication year: 2020

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Publication date: 2020-01-01

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Authors: Yoshihisa AKAMATSU, Humiko IMAMURA, Ryohei Nakao, Masuji Goto

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Landing page: https://doi.org/10.2208/jscejhe.76.2_i_1027

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Concepts: DNA, Visualization, DNA clamp, Biophysics, Chemistry, Molecular biology, Genetics, Biology, Computer science, Polymerase chain reaction, Gene, Reverse transcriptase, Artificial intelligence

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Cited by: 0

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abstract_inverted_index.地下水の流動は「トレーサー試験」によって把握するのが一般的である．本研究ではDNAを利用した新たなトレーサーを開発することを目的として，DNAトレーサーの作製とその有効性に関して基礎的な実験を行った．水中などの環境中のDNA分析ではフィルタリングしたものからDNAを抽出して，リアルタイム定量PCR分析をかけるため，むき出しのプラスミドDNAはトレーサーとして利用できないことがわかった．しかし，DNAをリポソームに封入した場合にはフィルターでのトラップが容易となり，DNAが96時間程度分解されないことが明らかとなった．また，このリポソーム封入DNAを用いて，1mの珪砂入り管路での地下水浸透実験を実施したところ，塩分とほぼ同様の動態を示し，地下水動態のトレーサーとして利用可能であることが示された．	0
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