Bioengineered Graphene Oxide Microcomposites Containing Metabolically Versatile Paracoccus sp. MKU1 for Enhanced Catechol Degradation Article Swipe
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· 2020
· Open Access
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· DOI: https://doi.org/10.1021/acsomega.0c01693
· OA: W3039727233
<i>Paracoccus sp</i>. MKU1, a metabolically versatile bacterium that encompasses diverse metabolic pathways in its genome for the degradation of aromatic compounds, was investigated for catechol bioremediation here for the first time to our knowledge. <i>Paracoccus</i> sp. MKU1 degraded catechol at an optimal pH of 7.5 and a temperature of 37 °C, wherein 100 mg/L catechol was completely mineralized in 96 h but required 192 h for complete mineralization of 500 mg/L catechol. While investigating the molecular mechanisms of its degradation potential, it was unveiled that <i>Paracoccus sp</i>. MKU1 employed both the ortho and meta pathways by inducing the expression of catechol 1,2-dioxygenase (C12O) and catechol 2,3-dioxygenase (C23O), respectively. <i>C23O</i> expression at transcriptional levels was significantly more abundant than <i>C12O</i>, which indicated that catechol degradation was primarily mediated by extradiol cleavage by MKU1. Furthermore, poly(MAA-<i>co</i>-BMA)-GO (PGO) microcomposites containing <i>Paracoccus sp</i>. MKU1 were synthesized, which degraded catechol (100 mg/L) completely within 48 h with excellent recycling performance for three cycles. Thus, PGO@<i>Paracoccus</i> microcomposites proved to be efficient in catechol degradation at not only faster rates but also with excellent recycling performances than free cells. These findings accomplish that <i>Paracoccus sp</i>. MKU1 could serve as a potential tool for bioremediation of catechol-polluted industrial wastewater and soil.
