Abstract Climate change due to the continuous increase in the release of green-house gasses associated with anthropogenic activity has made a significant impact on the sustainability of life on our planet. Methane (CH 4 ) is a green-house gas whose concentrations in the atmosphere are on the rise. CH 4 measurement is important for both the environment and the safety at the industrial and household level. Methanotrophs are distinguished for their unique characteristic of using CH 4 as the sole source of carbon and …