Boosting Mouse Defense against Lethal Toxoplasma gondii Infection with Full-Length and Soluble SAG1 Recombinant Protein Article Swipe
Xiang Li
,
Wei Yuan
,
Ting He
,
Ruiying Guo
,
Xiuxian Du
,
Yanhong He
,
Xuan Li
,
Saeed El‐Ashram
,
Ebtesam Al‐Olayan
,
Na Yang
,
Xiaoyu Sang
·
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.3390/vaccines11111678
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.3390/vaccines11111678
Toxoplasmosis is a major worldwide protozoan zoonosis. The surface antigen 1 (SAG1) of Toxoplasma gondii (T. gondii) has always been recognized as an ideal vaccine candidate antigen. However, the intact and soluble SAG1 protein is usually difficult to acquire in vitro, which is unfavorable for employing the recombinant protein as a vaccine candidate antigen. In the present study, we obtained the full-length SAG1 recombinant protein in soluble form by Escherichia coli Transetta (DE3) cells under optimized expression conditions. The immunogenicity and protective ability of this recombinant protein against T. gondii acute infection were evaluated in a mouse model. Monitoring changes in serum antibody levels and types, the presence of cytokines, and the rate of lymphocyte proliferation in vaccinated mice were used to assess humoral and cellular immune responses. Additional assessments were performed to determine the protective potency of the recombinant protein in combating T. gondii RH tachyzoites. It was found that the titers of both IgG2a and IgG2b were considerably greater in the immunized mice compared to the titers of IgG1 and IgG3. The levels of Th1-type cytokines (IFN-γ, IL-12p70, IL-2, and TNF-α) and Th2-type cytokines (IL-10) significantly increased when splenocytes from immunological group mice were treated with T. gondii lysate antigen. Compared to the control group, a recombinant protein substantially increased the longevity of infected mice, with an average death time prolonged by 14.50 ± 0.34 days (p < 0.0001). These findings suggest that the full-length and soluble SAG1 recombinant protein produced potent immune responses in mice and could be a preferred subunit vaccine candidate for T. gondii, offering a feasible option for vaccination against acute toxoplasmosis.
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3390/vaccines11111678
- https://www.mdpi.com/2076-393X/11/11/1678/pdf?version=1698917829
- OA Status
- gold
- Cited By
- 3
- References
- 47
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4388233851
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4388233851Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.3390/vaccines11111678Digital Object Identifier
- Title
-
Boosting Mouse Defense against Lethal Toxoplasma gondii Infection with Full-Length and Soluble SAG1 Recombinant ProteinWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2023Year of publication
- Publication date
-
2023-11-02Full publication date if available
- Authors
-
Xiang Li, Wei Yuan, Ting He, Ruiying Guo, Xiuxian Du, Yanhong He, Xuan Li, Saeed El‐Ashram, Ebtesam Al‐Olayan, Na Yang, Xiaoyu SangList of authors in order
- Landing page
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https://doi.org/10.3390/vaccines11111678Publisher landing page
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https://www.mdpi.com/2076-393X/11/11/1678/pdf?version=1698917829Direct link to full text PDF
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YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
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https://www.mdpi.com/2076-393X/11/11/1678/pdf?version=1698917829Direct OA link when available
- Concepts
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Toxoplasma gondii, Immunogenicity, Recombinant DNA, Antigen, Biology, Immune system, Toxoplasmosis, Antibody, Virology, Splenocyte, Titer, Immunology, Microbiology, Gene, BiochemistryTop concepts (fields/topics) attached by OpenAlex
- Cited by
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3Total citation count in OpenAlex
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2025: 3Per-year citation counts (last 5 years)
- References (count)
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47Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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