Bovine Sex Determining Region Y: Cloning, Optimized Expression, and Purification Article Swipe
Bijan Soleymani
,
Sayed Hassan Hafezian
,
Ghodratollah Rahimi Mianji
,
Kamran Mansouri
,
B. Chaharaein
,
Ahmad Tajehmiri
,
Mehdi Sharifi Tabar
,
Ali Mostafaie
·
YOU?
·
· 2016
· Open Access
·
· DOI: https://doi.org/10.1080/10495398.2016.1198796
YOU?
·
· 2016
· Open Access
·
· DOI: https://doi.org/10.1080/10495398.2016.1198796
Sex determining region Y gene (SRY) is located on Y chromosome and encodes a protein with 229 amino acids. In this study, ORF region of SRY with a length of 690 bp was synthesized using PCR and ligated to pET28a (+), then transformed in E.coli DH5α. E.coli BL21 (DE3) strain was chosen to express recombinant bovine SRY protein. A set of optimization steps was taken including different concentrations of IPTG, glucose, and temperatures at differed incubation times after the induction. Results showed that temperature points and different concentrations of IPTG and glucose had a significant effect (p
Related Topics
Concepts
Testis determining factor
lac operon
Recombinant DNA
Biology
Molecular biology
Escherichia coli
Cloning (programming)
Incubation
Gene
Y chromosome
Biochemistry
Programming language
Computer science
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1080/10495398.2016.1198796
- OA Status
- green
- Cited By
- 7
- References
- 42
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W2491905729
All OpenAlex metadata
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https://openalex.org/W2491905729Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1080/10495398.2016.1198796Digital Object Identifier
- Title
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Bovine Sex Determining Region Y: Cloning, Optimized Expression, and PurificationWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2016Year of publication
- Publication date
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2016-08-10Full publication date if available
- Authors
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Bijan Soleymani, Sayed Hassan Hafezian, Ghodratollah Rahimi Mianji, Kamran Mansouri, B. Chaharaein, Ahmad Tajehmiri, Mehdi Sharifi Tabar, Ali MostafaieList of authors in order
- Landing page
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https://doi.org/10.1080/10495398.2016.1198796Publisher landing page
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YesWhether a free full text is available
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greenOpen access status per OpenAlex
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https://figshare.com/articles/journal_contribution/Bovine_Sex_Determining_Region_Y_Cloning_Optimized_Expression_and_Purification/3568725Direct OA link when available
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Testis determining factor, lac operon, Recombinant DNA, Biology, Molecular biology, Escherichia coli, Cloning (programming), Incubation, Gene, Y chromosome, Biochemistry, Programming language, Computer scienceTop concepts (fields/topics) attached by OpenAlex
- Cited by
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7Total citation count in OpenAlex
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2021: 1, 2020: 3, 2019: 2, 2018: 1Per-year citation counts (last 5 years)
- References (count)
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42Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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