C-terminal kink formation is required for lateral gating in BamA Article Swipe

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Bama Gating Barrel (horology) Bacterial outer membrane Membrane Terminal (telecommunication) Bacteria Biophysics Core (optical fiber) Chemistry Biology Physics Materials science Biochemistry Computer science Escherichia coli Genetics Gene Optics Composite material Telecommunications

Karl Lundquist , Jeremy Bakelar , Nicholas Noinaj , James C. Gumbart ·

YOU? · · 2018 · Open Access · · DOI: https://doi.org/10.1073/pnas.1722530115 · OA: W2885624582

Significance Nearly all outer-membrane proteins (OMPs) in Gram-negative bacteria are inserted via the <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" overflow="scroll"> <mml:mi>β</mml:mi> </mml:math> -barrel assembly machinery (BAM) complex. Molecular dynamics simulations have revealed a persistent C-terminal strand kink of the core BAM component, BamA, which permits BamA to open laterally to the membrane. Experiments show that inhibiting kinking makes bacteria more susceptible to antibiotics. Kink-induced lateral gating may catalyze OMP insertion through a local disruption of the membrane, a pathway into the membrane, or both.