CapTrap-Seq cDNA library preparation for full-length RNA sequencing Article Swipe

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YOU? · · 2024 · Open Access · · DOI: https://doi.org/10.21203/rs.3.pex-2646/v1

Long-read RNA sequencing is crucial for generating precise and comprehensive annotations of eukaryotic genomes. Despite advancements in throughput and accuracy, achieving reliable end-to-end identification of RNA transcripts remains a challenge for long-read sequencing methods. To address this limitation, we developed CapTrap-seq, a cDNA library preparation method that combines the Cap-trapping strategy with oligo(dT) priming to detect 5' capped, full-length transcripts. CapTrap-seq is a competitive open-source, platform-agnostic RNA library preparation method for producing full-length transcript sequences.

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Computational Biology

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cDNA library Computational biology Complementary DNA RNA-Seq RNA Biology Genetics Molecular biology Computer science Gene Transcriptome Gene expression

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Type: preprint
Language: en
Landing Page: https://doi.org/10.21203/rs.3.pex-2646/v1
PDF: https://www.researchsquare.com/article/pex-2646/latest.pdf
OA Status: gold
Cited By: 2
References: 3
Related Works: 10
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DOI: https://doi.org/10.21203/rs.3.pex-2646/v1

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Title: CapTrap-Seq cDNA library preparation for full-length RNA sequencing

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Type: preprint

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Language: en

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Publication year: 2024

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Publication date: 2024-05-24

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Authors: Sílvia Carbonell Sala, Roderic Guigó

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Landing page: https://doi.org/10.21203/rs.3.pex-2646/v1

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Concepts: cDNA library, Computational biology, Complementary DNA, RNA-Seq, RNA, Biology, Genetics, Molecular biology, Computer science, Gene, Transcriptome, Gene expression

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Cited by: 2

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