Cell morphology as a biological fingerprint of chondrocyte phenotype in control and inflammatory conditions Article Swipe
Mischa Selig
,
Saman Azizi
,
Kathrin Walz
,
Jasmin Lauer
,
Bernd Rolauffs
,
Melanie L. Hart
·
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.3389/fimmu.2023.1102912
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.3389/fimmu.2023.1102912
Introduction Little is known how inflammatory processes quantitatively affect chondrocyte morphology and how single cell morphometric data could be used as a biological fingerprint of phenotype. Methods We investigated whether trainable high-throughput quantitative single cell morphology profiling combined with population-based gene expression analysis can be used to identify biological fingerprints that are discriminatory of control vs. inflammatory phenotypes. The shape of a large number of chondrocytes isolated from bovine healthy and human osteoarthritic (OA) cartilages was quantified under control and inflammatory (IL-1β) conditions using a trainable image analysis technique measuring a panel of cell shape descriptors (area, length, width, circularity, aspect ratio, roundness, solidity). The expression profiles of phenotypically relevant markers were quantified by ddPCR. Statistical analysis, multivariate data exploration, and projection-based modelling were used for identifying specific morphological fingerprints indicative of phenotype. Results Cell morphology was sensitive to both cell density and IL-1β. In both cell types, all shape descriptors correlated with expression of extracellular matrix (ECM)- and inflammatory-regulating genes. A hierarchical clustered image map revealed that individual samples sometimes responded differently in control or IL-1β conditions than the overall population. Despite these variances, discriminative projection-based modeling revealed distinct morphological fingerprints that discriminated between control and inflammatory chondrocyte phenotypes: the most essential morphological characteristics attributable to non-treated control cells was a higher cell aspect ratio in healthy bovine chondrocytes and roundness in OA human chondrocytes. In contrast, a higher circularity and width in healthy bovine chondrocytes and length and area in OA human chondrocytes indicated an inflammatory (IL-1β) phenotype. When comparing the two species/health conditions, bovine healthy and human OA chondrocytes exhibited comparable IL-1β-induced morphologies in roundness, a widely recognized marker of chondrocyte phenotype, and aspect ratio. Discussion Overall, cell morphology can be used as a biological fingerprint for describing chondrocyte phenotype. Quantitative single cell morphometry in conjunction with advanced methods for multivariate data analysis allows identifying morphological fingerprints that can discriminate between control and inflammatory chondrocyte phenotypes. This approach could be used to assess how culture conditions, inflammatory mediators, and therapeutic modulators regulate cell phenotype and function.
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3389/fimmu.2023.1102912
- https://www.frontiersin.org/articles/10.3389/fimmu.2023.1102912/pdf
- OA Status
- gold
- Cited By
- 17
- References
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- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4320495548
All OpenAlex metadata
Raw OpenAlex JSON
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- DOI
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https://doi.org/10.3389/fimmu.2023.1102912Digital Object Identifier
- Title
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Cell morphology as a biological fingerprint of chondrocyte phenotype in control and inflammatory conditionsWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2023Year of publication
- Publication date
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2023-02-13Full publication date if available
- Authors
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Mischa Selig, Saman Azizi, Kathrin Walz, Jasmin Lauer, Bernd Rolauffs, Melanie L. HartList of authors in order
- Landing page
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https://doi.org/10.3389/fimmu.2023.1102912Publisher landing page
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https://www.frontiersin.org/articles/10.3389/fimmu.2023.1102912/pdfDirect link to full text PDF
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YesWhether a free full text is available
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goldOpen access status per OpenAlex
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https://www.frontiersin.org/articles/10.3389/fimmu.2023.1102912/pdfDirect OA link when available
- Concepts
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Phenotype, Chondrocyte, Biology, Population, Cell type, Cell, Genetics, Cell biology, Gene, Anatomy, Cartilage, Medicine, Environmental healthTop concepts (fields/topics) attached by OpenAlex
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17Total citation count in OpenAlex
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2025: 4, 2024: 7, 2023: 6Per-year citation counts (last 5 years)
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110Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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