Cell-Type-Specific Surfaceome Profiling of 100-500 Isolated Cells using a Droplet-Based Magnetic Affinity Purification System Article Swipe
Amanda Lorentzian
,
Juliet M. Bartleson
,
Terence Ho
,
Zhichang Yang
,
Meena Choi
,
Tommy K. Cheung
,
Christopher M. Rose
,
William T. Yewdell
,
Ying Zhu
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1101/2025.03.13.643164
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1101/2025.03.13.643164
Cell surface proteins (CSPs) represent an important source of biomarkers and therapeutic targets. However, due to the inherent sensitivity limitations of existing technologies, tissue and cell-type-specific surfaceomes remain poorly characterized, especially in the context of human diseases. Herein, we develop nanoMAPS (nanoscale Magnetic Affinity Purification System), a miniaturized proteomic sample preparation method for surfaceome profiling of as few as 100-500 cells (1000× to 100,000× lower than existing technologies). We demonstrate that the miniaturization of magnetic bead-based affinity purification inside a single droplet can efficiently improve the recovery of surface proteins and reduce non-specific absorption of intracellular proteins. By applying nanoMAPS to human immune cells isolated from PBMCs, we demonstrate robust identification of both well-known cell-type-specific surface markers and candidate proteins. We establish nanoMAPS as a promising platform to expand surface proteomics from cultured cells to primary cells isolated from patients or mouse models.
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Metadata
- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.1101/2025.03.13.643164
- OA Status
- green
- References
- 47
- Related Works
- 10
- OpenAlex ID
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Raw OpenAlex JSON
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https://openalex.org/W4408495830Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1101/2025.03.13.643164Digital Object Identifier
- Title
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Cell-Type-Specific Surfaceome Profiling of 100-500 Isolated Cells using a Droplet-Based Magnetic Affinity Purification SystemWork title
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preprintOpenAlex work type
- Language
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enPrimary language
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2025Year of publication
- Publication date
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2025-03-15Full publication date if available
- Authors
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Amanda Lorentzian, Juliet M. Bartleson, Terence Ho, Zhichang Yang, Meena Choi, Tommy K. Cheung, Christopher M. Rose, William T. Yewdell, Ying ZhuList of authors in order
- Landing page
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https://doi.org/10.1101/2025.03.13.643164Publisher landing page
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YesWhether a free full text is available
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greenOpen access status per OpenAlex
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https://doi.org/10.1101/2025.03.13.643164Direct OA link when available
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Profiling (computer programming), Magnetic bead, Cell, Chemistry, Cell biology, Molecular biology, Computational biology, Chromatography, Biochemistry, Biology, Computer science, Operating systemTop concepts (fields/topics) attached by OpenAlex
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0Total citation count in OpenAlex
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47Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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| abstract_inverted_index.identification | 111 |
| abstract_inverted_index.technologies). | 68 |
| abstract_inverted_index.miniaturization | 73 |
| abstract_inverted_index.cell-type-specific | 26, 115 |
| cited_by_percentile_year | |
| countries_distinct_count | 0 |
| institutions_distinct_count | 9 |
| citation_normalized_percentile.value | 0.09187729 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |