Challenges and Opportunities in State‐of‐the‐Art Proteomics Analysis for Biomarker Development From Plasma Extracellular Vesicles Article Swipe
Panshak Dakup
,
Ivo Díaz Ludovico
,
Youngki You
,
Chaitra Rao
,
Javier E. Flores
,
Lisa Bramer
,
Marian Rewers
,
Bobbie‐Jo Webb‐Robertson
,
Thomas Metz
,
Raghavendra G. Mirmira
,
Emily K. Sims
,
Ernesto Nakayasu
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1002/pmic.70036
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1002/pmic.70036
Extracellular vesicles (EVs) are membrane‐bound particles secreted by cells, playing crucial roles in intercellular communication. The composition of EVs can undergo changes in response to stress and disease conditions, making them excellent biomarker candidates. However, extracting protein information from EVs can be challenging due to their low abundance in complex biofluids and copurification with contaminant proteins and particles. Techniques to enrich EVs have their strengths and limitations, without one being able to purify EVs to complete homogeneity. This can lead to compromised recovery rates and increased complexity, making data interpretation difficult. In this viewpoint article, we explore the concept that better characterization of EV composition, followed by quantification of EV proteins in complex samples, might be a more viable route for biomarker development. Mass spectrometers can provide reproducible deep coverage of the EV proteome, despite sample impurities. This paradigm shift presents opportunities to integrate advanced bioinformatics tools to refine the EV proteome landscape, identify novel biomarkers, and streamline validation processes in biomarker development. By focusing on leveraging technology rather than achieving absolute purity, this approach can transform current practices and open opportunities for robust biomarker discovery. Herein, we highlight not only such opportunities but also challenges to implement this concept. Summary Extracellular vesicles (EVs) have enormous potential as biomarkers of diseases, as they can carry signatures of the cells they are derived from and the pathogenesis process. Biofluids, such as blood plasma, are highly complex and contain many components with physicochemical properties similar to those of EVs, making it challenging to obtain pure EV fractions. Challenges in obtaining pure preparations represent a main hurdle for studying EVs, and their components are potential biomarkers. This article explores the concept of studying EV proteins within complex samples, discussing opportunities and needs to move this field forward.
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- https://openalex.org/W4414248278
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https://openalex.org/W4414248278Canonical identifier for this work in OpenAlex
- DOI
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- Title
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Challenges and Opportunities in State‐of‐the‐Art Proteomics Analysis for Biomarker Development From Plasma Extracellular VesiclesWork title
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articleOpenAlex work type
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enPrimary language
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2025Year of publication
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2025-09-16Full publication date if available
- Authors
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Panshak Dakup, Ivo Díaz Ludovico, Youngki You, Chaitra Rao, Javier E. Flores, Lisa Bramer, Marian Rewers, Bobbie‐Jo Webb‐Robertson, Thomas Metz, Raghavendra G. Mirmira, Emily K. Sims, Ernesto NakayasuList of authors in order
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https://doi.org/10.1002/pmic.70036Publisher landing page
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hybridOpen access status per OpenAlex
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https://doi.org/10.1002/pmic.70036Direct OA link when available
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