Changes in Cell Morphology and Actin Organization in Embryonic Stem Cells Cultured under Different Conditions Article Swipe
Younes Farhangi Barooji
,
Kasper Graves Hvid
,
Irene Istúriz Petitjean
,
Joshua M. Brickman
,
Lene B. Oddershede
,
Poul Martin Bendix
·
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.3390/cells10112859
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.3390/cells10112859
The cellular cytoskeleton provides the cell with a mechanical rigidity that allows mechanical interaction between cells and the extracellular environment. The actin structure plays a key role in mechanical events such as motility or the establishment of cell polarity. From the earliest stages of development, as represented by the ex vivo expansion of naïve embryonic stem cells (ESCs), the critical mechanical role of the actin structure is becoming recognized as a vital cue for correct segregation and lineage control of cells and as a regulatory structure that controls several transcription factors. Naïve ESCs have a characteristic morphology, and the ultrastructure that underlies this condition remains to be further investigated. Here, we investigate the 3D actin cytoskeleton of naïve mouse ESCs using super-resolution optical reconstruction microscopy (STORM). We investigate the morphological, cytoskeletal, and mechanical changes in cells cultured in 2i or Serum/LIF media reflecting, respectively, a homogeneous preimplantation cell state and a state that is closer to embarking on differentiation. STORM imaging showed that the peripheral actin structure undergoes a dramatic change between the two culturing conditions. We also detected micro-rheological differences in the cell periphery between the cells cultured in these two media correlating well with the observed nano-architecture of the ESCs in the two different culture conditions. These results pave the way for linking physical properties and cytoskeletal architecture to cell morphology during early development.
Related Topics
Concepts
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3390/cells10112859
- https://www.mdpi.com/2073-4409/10/11/2859/pdf?version=1635760753
- OA Status
- gold
- Cited By
- 4
- References
- 57
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3209663610
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W3209663610Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.3390/cells10112859Digital Object Identifier
- Title
-
Changes in Cell Morphology and Actin Organization in Embryonic Stem Cells Cultured under Different ConditionsWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2021Year of publication
- Publication date
-
2021-10-23Full publication date if available
- Authors
-
Younes Farhangi Barooji, Kasper Graves Hvid, Irene Istúriz Petitjean, Joshua M. Brickman, Lene B. Oddershede, Poul Martin BendixList of authors in order
- Landing page
-
https://doi.org/10.3390/cells10112859Publisher landing page
- PDF URL
-
https://www.mdpi.com/2073-4409/10/11/2859/pdf?version=1635760753Direct link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
-
https://www.mdpi.com/2073-4409/10/11/2859/pdf?version=1635760753Direct OA link when available
- Concepts
-
Cell biology, Cytoskeleton, Embryonic stem cell, Actin, Biology, Motility, Myosin, Stem cell, Cellular differentiation, Cell, Chemistry, Genetics, GeneTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
4Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 1, 2024: 1, 2023: 2Per-year citation counts (last 5 years)
- References (count)
-
57Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
Full payload
| id | https://openalex.org/W3209663610 |
|---|---|
| doi | https://doi.org/10.3390/cells10112859 |
| ids.doi | https://doi.org/10.3390/cells10112859 |
| ids.mag | 3209663610 |
| ids.pmid | https://pubmed.ncbi.nlm.nih.gov/34831083 |
| ids.openalex | https://openalex.org/W3209663610 |
| fwci | 0.3911391 |
| mesh[0].qualifier_ui | Q000378 |
| mesh[0].descriptor_ui | D008841 |
| mesh[0].is_major_topic | False |
| mesh[0].qualifier_name | metabolism |
| mesh[0].descriptor_name | Actin Cytoskeleton |
| mesh[1].qualifier_ui | Q000378 |
| mesh[1].descriptor_ui | D000199 |
| mesh[1].is_major_topic | False |
| mesh[1].qualifier_name | metabolism |
| mesh[1].descriptor_name | Actins |
| mesh[2].qualifier_ui | |
| mesh[2].descriptor_ui | D000818 |
| mesh[2].is_major_topic | False |
| mesh[2].qualifier_name | |
| mesh[2].descriptor_name | Animals |
| mesh[3].qualifier_ui | |
| mesh[3].descriptor_ui | D049109 |
| mesh[3].is_major_topic | False |
| mesh[3].qualifier_name | |
| mesh[3].descriptor_name | Cell Proliferation |
| mesh[4].qualifier_ui | |
| mesh[4].descriptor_ui | D048430 |
| mesh[4].is_major_topic | True |
| mesh[4].qualifier_name | |
| mesh[4].descriptor_name | Cell Shape |
| mesh[5].qualifier_ui | |
| mesh[5].descriptor_ui | D002478 |
| mesh[5].is_major_topic | False |
| mesh[5].qualifier_name | |
| mesh[5].descriptor_name | Cells, Cultured |
| mesh[6].qualifier_ui | |
| mesh[6].descriptor_ui | D004548 |
| mesh[6].is_major_topic | False |
| mesh[6].qualifier_name | |
| mesh[6].descriptor_name | Elasticity |
| mesh[7].qualifier_ui | |
| mesh[7].descriptor_ui | D021621 |
| mesh[7].is_major_topic | False |
| mesh[7].qualifier_name | |
| mesh[7].descriptor_name | Imaging, Three-Dimensional |
| mesh[8].qualifier_ui | |
| mesh[8].descriptor_ui | D051379 |
| mesh[8].is_major_topic | False |
| mesh[8].qualifier_name | |
| mesh[8].descriptor_name | Mice |
| mesh[9].qualifier_ui | Q000166 |
| mesh[9].descriptor_ui | D000066450 |
| mesh[9].is_major_topic | False |
| mesh[9].qualifier_name | cytology |
| mesh[9].descriptor_name | Mouse Embryonic Stem Cells |
| mesh[10].qualifier_ui | Q000737 |
| mesh[10].descriptor_ui | D053758 |
| mesh[10].is_major_topic | False |
| mesh[10].qualifier_name | chemistry |
| mesh[10].descriptor_name | Nanoparticles |
| mesh[11].qualifier_ui | |
| mesh[11].descriptor_ui | D014783 |
| mesh[11].is_major_topic | False |
| mesh[11].qualifier_name | |
| mesh[11].descriptor_name | Viscosity |
| mesh[12].qualifier_ui | Q000378 |
| mesh[12].descriptor_ui | D008841 |
| mesh[12].is_major_topic | False |
| mesh[12].qualifier_name | metabolism |
| mesh[12].descriptor_name | Actin Cytoskeleton |
| mesh[13].qualifier_ui | Q000378 |
| mesh[13].descriptor_ui | D000199 |
| mesh[13].is_major_topic | False |
| mesh[13].qualifier_name | metabolism |
| mesh[13].descriptor_name | Actins |
| mesh[14].qualifier_ui | |
| mesh[14].descriptor_ui | D000818 |
| mesh[14].is_major_topic | False |
| mesh[14].qualifier_name | |
| mesh[14].descriptor_name | Animals |
| mesh[15].qualifier_ui | |
| mesh[15].descriptor_ui | D049109 |
| mesh[15].is_major_topic | False |
| mesh[15].qualifier_name | |
| mesh[15].descriptor_name | Cell Proliferation |
| mesh[16].qualifier_ui | |
| mesh[16].descriptor_ui | D048430 |
| mesh[16].is_major_topic | True |
| mesh[16].qualifier_name | |
| mesh[16].descriptor_name | Cell Shape |
| mesh[17].qualifier_ui | |
| mesh[17].descriptor_ui | D002478 |
| mesh[17].is_major_topic | False |
| mesh[17].qualifier_name | |
| mesh[17].descriptor_name | Cells, Cultured |
| mesh[18].qualifier_ui | |
| mesh[18].descriptor_ui | D004548 |
| mesh[18].is_major_topic | False |
| mesh[18].qualifier_name | |
| mesh[18].descriptor_name | Elasticity |
| mesh[19].qualifier_ui | |
| mesh[19].descriptor_ui | D021621 |
| mesh[19].is_major_topic | False |
| mesh[19].qualifier_name | |
| mesh[19].descriptor_name | Imaging, Three-Dimensional |
| mesh[20].qualifier_ui | |
| mesh[20].descriptor_ui | D051379 |
| mesh[20].is_major_topic | False |
| mesh[20].qualifier_name | |
| mesh[20].descriptor_name | Mice |
| mesh[21].qualifier_ui | Q000166 |
| mesh[21].descriptor_ui | D000066450 |
| mesh[21].is_major_topic | False |
| mesh[21].qualifier_name | cytology |
| mesh[21].descriptor_name | Mouse Embryonic Stem Cells |
| mesh[22].qualifier_ui | Q000737 |
| mesh[22].descriptor_ui | D053758 |
| mesh[22].is_major_topic | False |
| mesh[22].qualifier_name | chemistry |
| mesh[22].descriptor_name | Nanoparticles |
| mesh[23].qualifier_ui | |
| mesh[23].descriptor_ui | D014783 |
| mesh[23].is_major_topic | False |
| mesh[23].qualifier_name | |
| mesh[23].descriptor_name | Viscosity |
| type | article |
| title | Changes in Cell Morphology and Actin Organization in Embryonic Stem Cells Cultured under Different Conditions |
| awards[0].id | https://openalex.org/G4807775794 |
| awards[0].funder_id | https://openalex.org/F4320320943 |
| awards[0].display_name | |
| awards[0].funder_award_id | DNRF116 (StemPhys), |
| awards[0].funder_display_name | Danmarks Grundforskningsfond |
| biblio.issue | 11 |
| biblio.volume | 10 |
| biblio.last_page | 2859 |
| biblio.first_page | 2859 |
| topics[0].id | https://openalex.org/T10379 |
| topics[0].field.id | https://openalex.org/fields/13 |
| topics[0].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[0].score | 0.9998999834060669 |
| topics[0].domain.id | https://openalex.org/domains/1 |
| topics[0].domain.display_name | Life Sciences |
| topics[0].subfield.id | https://openalex.org/subfields/1307 |
| topics[0].subfield.display_name | Cell Biology |
| topics[0].display_name | Cellular Mechanics and Interactions |
| topics[1].id | https://openalex.org/T11190 |
| topics[1].field.id | https://openalex.org/fields/22 |
| topics[1].field.display_name | Engineering |
| topics[1].score | 0.9994000196456909 |
| topics[1].domain.id | https://openalex.org/domains/3 |
| topics[1].domain.display_name | Physical Sciences |
| topics[1].subfield.id | https://openalex.org/subfields/2204 |
| topics[1].subfield.display_name | Biomedical Engineering |
| topics[1].display_name | 3D Printing in Biomedical Research |
| topics[2].id | https://openalex.org/T11255 |
| topics[2].field.id | https://openalex.org/fields/22 |
| topics[2].field.display_name | Engineering |
| topics[2].score | 0.9993000030517578 |
| topics[2].domain.id | https://openalex.org/domains/3 |
| topics[2].domain.display_name | Physical Sciences |
| topics[2].subfield.id | https://openalex.org/subfields/2204 |
| topics[2].subfield.display_name | Biomedical Engineering |
| topics[2].display_name | Microfluidic and Bio-sensing Technologies |
| funders[0].id | https://openalex.org/F4320320943 |
| funders[0].ror | https://ror.org/00znyv691 |
| funders[0].display_name | Danmarks Grundforskningsfond |
| is_xpac | False |
| apc_list.value | 2200 |
| apc_list.currency | CHF |
| apc_list.value_usd | 2382 |
| apc_paid.value | 2200 |
| apc_paid.currency | CHF |
| apc_paid.value_usd | 2382 |
| concepts[0].id | https://openalex.org/C95444343 |
| concepts[0].level | 1 |
| concepts[0].score | 0.7674393653869629 |
| concepts[0].wikidata | https://www.wikidata.org/wiki/Q7141 |
| concepts[0].display_name | Cell biology |
| concepts[1].id | https://openalex.org/C142669718 |
| concepts[1].level | 3 |
| concepts[1].score | 0.7575620412826538 |
| concepts[1].wikidata | https://www.wikidata.org/wiki/Q154626 |
| concepts[1].display_name | Cytoskeleton |
| concepts[2].id | https://openalex.org/C145103041 |
| concepts[2].level | 3 |
| concepts[2].score | 0.665274441242218 |
| concepts[2].wikidata | https://www.wikidata.org/wiki/Q1151519 |
| concepts[2].display_name | Embryonic stem cell |
| concepts[3].id | https://openalex.org/C125705527 |
| concepts[3].level | 2 |
| concepts[3].score | 0.602576494216919 |
| concepts[3].wikidata | https://www.wikidata.org/wiki/Q185269 |
| concepts[3].display_name | Actin |
| concepts[4].id | https://openalex.org/C86803240 |
| concepts[4].level | 0 |
| concepts[4].score | 0.5017812252044678 |
| concepts[4].wikidata | https://www.wikidata.org/wiki/Q420 |
| concepts[4].display_name | Biology |
| concepts[5].id | https://openalex.org/C58207958 |
| concepts[5].level | 2 |
| concepts[5].score | 0.47540172934532166 |
| concepts[5].wikidata | https://www.wikidata.org/wiki/Q33596 |
| concepts[5].display_name | Motility |
| concepts[6].id | https://openalex.org/C6997183 |
| concepts[6].level | 2 |
| concepts[6].score | 0.474003404378891 |
| concepts[6].wikidata | https://www.wikidata.org/wiki/Q271727 |
| concepts[6].display_name | Myosin |
| concepts[7].id | https://openalex.org/C28328180 |
| concepts[7].level | 2 |
| concepts[7].score | 0.47377681732177734 |
| concepts[7].wikidata | https://www.wikidata.org/wiki/Q48196 |
| concepts[7].display_name | Stem cell |
| concepts[8].id | https://openalex.org/C148738053 |
| concepts[8].level | 3 |
| concepts[8].score | 0.4581211805343628 |
| concepts[8].wikidata | https://www.wikidata.org/wiki/Q210861 |
| concepts[8].display_name | Cellular differentiation |
| concepts[9].id | https://openalex.org/C1491633281 |
| concepts[9].level | 2 |
| concepts[9].score | 0.4486520290374756 |
| concepts[9].wikidata | https://www.wikidata.org/wiki/Q7868 |
| concepts[9].display_name | Cell |
| concepts[10].id | https://openalex.org/C185592680 |
| concepts[10].level | 0 |
| concepts[10].score | 0.3399757146835327 |
| concepts[10].wikidata | https://www.wikidata.org/wiki/Q2329 |
| concepts[10].display_name | Chemistry |
| concepts[11].id | https://openalex.org/C54355233 |
| concepts[11].level | 1 |
| concepts[11].score | 0.10080394148826599 |
| concepts[11].wikidata | https://www.wikidata.org/wiki/Q7162 |
| concepts[11].display_name | Genetics |
| concepts[12].id | https://openalex.org/C104317684 |
| concepts[12].level | 2 |
| concepts[12].score | 0.0 |
| concepts[12].wikidata | https://www.wikidata.org/wiki/Q7187 |
| concepts[12].display_name | Gene |
| keywords[0].id | https://openalex.org/keywords/cell-biology |
| keywords[0].score | 0.7674393653869629 |
| keywords[0].display_name | Cell biology |
| keywords[1].id | https://openalex.org/keywords/cytoskeleton |
| keywords[1].score | 0.7575620412826538 |
| keywords[1].display_name | Cytoskeleton |
| keywords[2].id | https://openalex.org/keywords/embryonic-stem-cell |
| keywords[2].score | 0.665274441242218 |
| keywords[2].display_name | Embryonic stem cell |
| keywords[3].id | https://openalex.org/keywords/actin |
| keywords[3].score | 0.602576494216919 |
| keywords[3].display_name | Actin |
| keywords[4].id | https://openalex.org/keywords/biology |
| keywords[4].score | 0.5017812252044678 |
| keywords[4].display_name | Biology |
| keywords[5].id | https://openalex.org/keywords/motility |
| keywords[5].score | 0.47540172934532166 |
| keywords[5].display_name | Motility |
| keywords[6].id | https://openalex.org/keywords/myosin |
| keywords[6].score | 0.474003404378891 |
| keywords[6].display_name | Myosin |
| keywords[7].id | https://openalex.org/keywords/stem-cell |
| keywords[7].score | 0.47377681732177734 |
| keywords[7].display_name | Stem cell |
| keywords[8].id | https://openalex.org/keywords/cellular-differentiation |
| keywords[8].score | 0.4581211805343628 |
| keywords[8].display_name | Cellular differentiation |
| keywords[9].id | https://openalex.org/keywords/cell |
| keywords[9].score | 0.4486520290374756 |
| keywords[9].display_name | Cell |
| keywords[10].id | https://openalex.org/keywords/chemistry |
| keywords[10].score | 0.3399757146835327 |
| keywords[10].display_name | Chemistry |
| keywords[11].id | https://openalex.org/keywords/genetics |
| keywords[11].score | 0.10080394148826599 |
| keywords[11].display_name | Genetics |
| language | en |
| locations[0].id | doi:10.3390/cells10112859 |
| locations[0].is_oa | True |
| locations[0].source.id | https://openalex.org/S2736626452 |
| locations[0].source.issn | 2073-4409 |
| locations[0].source.type | journal |
| locations[0].source.is_oa | True |
| locations[0].source.issn_l | 2073-4409 |
| locations[0].source.is_core | True |
| locations[0].source.is_in_doaj | True |
| locations[0].source.display_name | Cells |
| locations[0].source.host_organization | https://openalex.org/P4310310987 |
| locations[0].source.host_organization_name | Multidisciplinary Digital Publishing Institute |
| locations[0].source.host_organization_lineage | https://openalex.org/P4310310987 |
| locations[0].source.host_organization_lineage_names | Multidisciplinary Digital Publishing Institute |
| locations[0].license | cc-by |
| locations[0].pdf_url | https://www.mdpi.com/2073-4409/10/11/2859/pdf?version=1635760753 |
| locations[0].version | publishedVersion |
| locations[0].raw_type | journal-article |
| locations[0].license_id | https://openalex.org/licenses/cc-by |
| locations[0].is_accepted | True |
| locations[0].is_published | True |
| locations[0].raw_source_name | Cells |
| locations[0].landing_page_url | https://doi.org/10.3390/cells10112859 |
| locations[1].id | pmid:34831083 |
| locations[1].is_oa | False |
| locations[1].source.id | https://openalex.org/S4306525036 |
| locations[1].source.issn | |
| locations[1].source.type | repository |
| locations[1].source.is_oa | False |
| locations[1].source.issn_l | |
| locations[1].source.is_core | False |
| locations[1].source.is_in_doaj | False |
| locations[1].source.display_name | PubMed |
| locations[1].source.host_organization | https://openalex.org/I1299303238 |
| locations[1].source.host_organization_name | National Institutes of Health |
| locations[1].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[1].license | |
| locations[1].pdf_url | |
| locations[1].version | publishedVersion |
| locations[1].raw_type | |
| locations[1].license_id | |
| locations[1].is_accepted | True |
| locations[1].is_published | True |
| locations[1].raw_source_name | Cells |
| locations[1].landing_page_url | https://pubmed.ncbi.nlm.nih.gov/34831083 |
| locations[2].id | pmh:oai:doaj.org/article:a7a3bdb97c8d4c66a9b705b854a9fb01 |
| locations[2].is_oa | True |
| locations[2].source.id | https://openalex.org/S4306401280 |
| locations[2].source.issn | |
| locations[2].source.type | repository |
| locations[2].source.is_oa | False |
| locations[2].source.issn_l | |
| locations[2].source.is_core | False |
| locations[2].source.is_in_doaj | False |
| locations[2].source.display_name | DOAJ (DOAJ: Directory of Open Access Journals) |
| locations[2].source.host_organization | |
| locations[2].source.host_organization_name | |
| locations[2].license | cc-by-sa |
| locations[2].pdf_url | |
| locations[2].version | submittedVersion |
| locations[2].raw_type | article |
| locations[2].license_id | https://openalex.org/licenses/cc-by-sa |
| locations[2].is_accepted | False |
| locations[2].is_published | False |
| locations[2].raw_source_name | Cells, Vol 10, Iss 11, p 2859 (2021) |
| locations[2].landing_page_url | https://doaj.org/article/a7a3bdb97c8d4c66a9b705b854a9fb01 |
| locations[3].id | pmh:oai:mdpi.com:/2073-4409/10/11/2859/ |
| locations[3].is_oa | True |
| locations[3].source.id | https://openalex.org/S4306400947 |
| locations[3].source.issn | |
| locations[3].source.type | repository |
| locations[3].source.is_oa | True |
| locations[3].source.issn_l | |
| locations[3].source.is_core | False |
| locations[3].source.is_in_doaj | False |
| locations[3].source.display_name | MDPI (MDPI AG) |
| locations[3].source.host_organization | https://openalex.org/I4210097602 |
| locations[3].source.host_organization_name | Multidisciplinary Digital Publishing Institute (Switzerland) |
| locations[3].source.host_organization_lineage | https://openalex.org/I4210097602 |
| locations[3].license | cc-by |
| locations[3].pdf_url | |
| locations[3].version | submittedVersion |
| locations[3].raw_type | Text |
| locations[3].license_id | https://openalex.org/licenses/cc-by |
| locations[3].is_accepted | False |
| locations[3].is_published | False |
| locations[3].raw_source_name | Cells; Volume 10; Issue 11; Pages: 2859 |
| locations[3].landing_page_url | https://dx.doi.org/10.3390/cells10112859 |
| locations[4].id | pmh:oai:pubmedcentral.nih.gov:8616278 |
| locations[4].is_oa | True |
| locations[4].source.id | https://openalex.org/S2764455111 |
| locations[4].source.issn | |
| locations[4].source.type | repository |
| locations[4].source.is_oa | False |
| locations[4].source.issn_l | |
| locations[4].source.is_core | False |
| locations[4].source.is_in_doaj | False |
| locations[4].source.display_name | PubMed Central |
| locations[4].source.host_organization | https://openalex.org/I1299303238 |
| locations[4].source.host_organization_name | National Institutes of Health |
| locations[4].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[4].license | other-oa |
| locations[4].pdf_url | |
| locations[4].version | submittedVersion |
| locations[4].raw_type | Text |
| locations[4].license_id | https://openalex.org/licenses/other-oa |
| locations[4].is_accepted | False |
| locations[4].is_published | False |
| locations[4].raw_source_name | Cells |
| locations[4].landing_page_url | https://www.ncbi.nlm.nih.gov/pmc/articles/8616278 |
| indexed_in | crossref, doaj, pubmed |
| authorships[0].author.id | https://openalex.org/A5036013914 |
| authorships[0].author.orcid | https://orcid.org/0000-0002-0907-9339 |
| authorships[0].author.display_name | Younes Farhangi Barooji |
| authorships[0].countries | DK |
| authorships[0].affiliations[0].institution_ids | https://openalex.org/I124055696 |
| authorships[0].affiliations[0].raw_affiliation_string | Niels Bohr Institute, University of Copenhagen, 2100 Copenhagen, Denmark |
| authorships[0].institutions[0].id | https://openalex.org/I124055696 |
| authorships[0].institutions[0].ror | https://ror.org/035b05819 |
| authorships[0].institutions[0].type | education |
| authorships[0].institutions[0].lineage | https://openalex.org/I124055696 |
| authorships[0].institutions[0].country_code | DK |
| authorships[0].institutions[0].display_name | University of Copenhagen |
| authorships[0].author_position | first |
| authorships[0].raw_author_name | Younes F. Barooji |
| authorships[0].is_corresponding | False |
| authorships[0].raw_affiliation_strings | Niels Bohr Institute, University of Copenhagen, 2100 Copenhagen, Denmark |
| authorships[1].author.id | https://openalex.org/A5013252115 |
| authorships[1].author.orcid | https://orcid.org/0000-0002-7870-5444 |
| authorships[1].author.display_name | Kasper Graves Hvid |
| authorships[1].countries | DK |
| authorships[1].affiliations[0].institution_ids | https://openalex.org/I124055696 |
| authorships[1].affiliations[0].raw_affiliation_string | Niels Bohr Institute, University of Copenhagen, 2100 Copenhagen, Denmark |
| authorships[1].institutions[0].id | https://openalex.org/I124055696 |
| authorships[1].institutions[0].ror | https://ror.org/035b05819 |
| authorships[1].institutions[0].type | education |
| authorships[1].institutions[0].lineage | https://openalex.org/I124055696 |
| authorships[1].institutions[0].country_code | DK |
| authorships[1].institutions[0].display_name | University of Copenhagen |
| authorships[1].author_position | middle |
| authorships[1].raw_author_name | Kasper G. Hvid |
| authorships[1].is_corresponding | False |
| authorships[1].raw_affiliation_strings | Niels Bohr Institute, University of Copenhagen, 2100 Copenhagen, Denmark |
| authorships[2].author.id | https://openalex.org/A5042633582 |
| authorships[2].author.orcid | https://orcid.org/0000-0001-9003-5781 |
| authorships[2].author.display_name | Irene Istúriz Petitjean |
| authorships[2].countries | DK |
| authorships[2].affiliations[0].institution_ids | https://openalex.org/I124055696 |
| authorships[2].affiliations[0].raw_affiliation_string | Niels Bohr Institute, University of Copenhagen, 2100 Copenhagen, Denmark |
| authorships[2].institutions[0].id | https://openalex.org/I124055696 |
| authorships[2].institutions[0].ror | https://ror.org/035b05819 |
| authorships[2].institutions[0].type | education |
| authorships[2].institutions[0].lineage | https://openalex.org/I124055696 |
| authorships[2].institutions[0].country_code | DK |
| authorships[2].institutions[0].display_name | University of Copenhagen |
| authorships[2].author_position | middle |
| authorships[2].raw_author_name | Irene Istúriz Petitjean |
| authorships[2].is_corresponding | False |
| authorships[2].raw_affiliation_strings | Niels Bohr Institute, University of Copenhagen, 2100 Copenhagen, Denmark |
| authorships[3].author.id | https://openalex.org/A5073502369 |
| authorships[3].author.orcid | https://orcid.org/0000-0003-1580-7491 |
| authorships[3].author.display_name | Joshua M. Brickman |
| authorships[3].countries | DK |
| authorships[3].affiliations[0].institution_ids | https://openalex.org/I2801134892 |
| authorships[3].affiliations[0].raw_affiliation_string | The Novo Nordisk Foundation Center for Stem Cell Biology, 2200 Copenhagen, Denmark |
| authorships[3].institutions[0].id | https://openalex.org/I2801134892 |
| authorships[3].institutions[0].ror | https://ror.org/04txyc737 |
| authorships[3].institutions[0].type | facility |
| authorships[3].institutions[0].lineage | https://openalex.org/I2801134892 |
| authorships[3].institutions[0].country_code | DK |
| authorships[3].institutions[0].display_name | Novo Nordisk Foundation |
| authorships[3].author_position | middle |
| authorships[3].raw_author_name | Joshua M. Brickman |
| authorships[3].is_corresponding | False |
| authorships[3].raw_affiliation_strings | The Novo Nordisk Foundation Center for Stem Cell Biology, 2200 Copenhagen, Denmark |
| authorships[4].author.id | https://openalex.org/A5070844214 |
| authorships[4].author.orcid | https://orcid.org/0000-0003-2923-2844 |
| authorships[4].author.display_name | Lene B. Oddershede |
| authorships[4].countries | DK |
| authorships[4].affiliations[0].institution_ids | https://openalex.org/I124055696 |
| authorships[4].affiliations[0].raw_affiliation_string | Niels Bohr Institute, University of Copenhagen, 2100 Copenhagen, Denmark |
| authorships[4].institutions[0].id | https://openalex.org/I124055696 |
| authorships[4].institutions[0].ror | https://ror.org/035b05819 |
| authorships[4].institutions[0].type | education |
| authorships[4].institutions[0].lineage | https://openalex.org/I124055696 |
| authorships[4].institutions[0].country_code | DK |
| authorships[4].institutions[0].display_name | University of Copenhagen |
| authorships[4].author_position | middle |
| authorships[4].raw_author_name | Lene B. Oddershede |
| authorships[4].is_corresponding | True |
| authorships[4].raw_affiliation_strings | Niels Bohr Institute, University of Copenhagen, 2100 Copenhagen, Denmark |
| authorships[5].author.id | https://openalex.org/A5029719845 |
| authorships[5].author.orcid | https://orcid.org/0000-0001-8838-8887 |
| authorships[5].author.display_name | Poul Martin Bendix |
| authorships[5].countries | DK |
| authorships[5].affiliations[0].institution_ids | https://openalex.org/I124055696 |
| authorships[5].affiliations[0].raw_affiliation_string | Niels Bohr Institute, University of Copenhagen, 2100 Copenhagen, Denmark |
| authorships[5].institutions[0].id | https://openalex.org/I124055696 |
| authorships[5].institutions[0].ror | https://ror.org/035b05819 |
| authorships[5].institutions[0].type | education |
| authorships[5].institutions[0].lineage | https://openalex.org/I124055696 |
| authorships[5].institutions[0].country_code | DK |
| authorships[5].institutions[0].display_name | University of Copenhagen |
| authorships[5].author_position | last |
| authorships[5].raw_author_name | Poul M. Bendix |
| authorships[5].is_corresponding | True |
| authorships[5].raw_affiliation_strings | Niels Bohr Institute, University of Copenhagen, 2100 Copenhagen, Denmark |
| has_content.pdf | True |
| has_content.grobid_xml | True |
| is_paratext | False |
| open_access.is_oa | True |
| open_access.oa_url | https://www.mdpi.com/2073-4409/10/11/2859/pdf?version=1635760753 |
| open_access.oa_status | gold |
| open_access.any_repository_has_fulltext | False |
| created_date | 2025-10-10T00:00:00 |
| display_name | Changes in Cell Morphology and Actin Organization in Embryonic Stem Cells Cultured under Different Conditions |
| has_fulltext | True |
| is_retracted | False |
| updated_date | 2025-11-06T03:46:38.306776 |
| primary_topic.id | https://openalex.org/T10379 |
| primary_topic.field.id | https://openalex.org/fields/13 |
| primary_topic.field.display_name | Biochemistry, Genetics and Molecular Biology |
| primary_topic.score | 0.9998999834060669 |
| primary_topic.domain.id | https://openalex.org/domains/1 |
| primary_topic.domain.display_name | Life Sciences |
| primary_topic.subfield.id | https://openalex.org/subfields/1307 |
| primary_topic.subfield.display_name | Cell Biology |
| primary_topic.display_name | Cellular Mechanics and Interactions |
| related_works | https://openalex.org/W240046108, https://openalex.org/W2397751340, https://openalex.org/W2613165074, https://openalex.org/W4406202825, https://openalex.org/W2163215382, https://openalex.org/W2415424592, https://openalex.org/W2113186347, https://openalex.org/W2773405305, https://openalex.org/W4404473509, https://openalex.org/W2000761246 |
| cited_by_count | 4 |
| counts_by_year[0].year | 2025 |
| counts_by_year[0].cited_by_count | 1 |
| counts_by_year[1].year | 2024 |
| counts_by_year[1].cited_by_count | 1 |
| counts_by_year[2].year | 2023 |
| counts_by_year[2].cited_by_count | 2 |
| locations_count | 5 |
| best_oa_location.id | doi:10.3390/cells10112859 |
| best_oa_location.is_oa | True |
| best_oa_location.source.id | https://openalex.org/S2736626452 |
| best_oa_location.source.issn | 2073-4409 |
| best_oa_location.source.type | journal |
| best_oa_location.source.is_oa | True |
| best_oa_location.source.issn_l | 2073-4409 |
| best_oa_location.source.is_core | True |
| best_oa_location.source.is_in_doaj | True |
| best_oa_location.source.display_name | Cells |
| best_oa_location.source.host_organization | https://openalex.org/P4310310987 |
| best_oa_location.source.host_organization_name | Multidisciplinary Digital Publishing Institute |
| best_oa_location.source.host_organization_lineage | https://openalex.org/P4310310987 |
| best_oa_location.source.host_organization_lineage_names | Multidisciplinary Digital Publishing Institute |
| best_oa_location.license | cc-by |
| best_oa_location.pdf_url | https://www.mdpi.com/2073-4409/10/11/2859/pdf?version=1635760753 |
| best_oa_location.version | publishedVersion |
| best_oa_location.raw_type | journal-article |
| best_oa_location.license_id | https://openalex.org/licenses/cc-by |
| best_oa_location.is_accepted | True |
| best_oa_location.is_published | True |
| best_oa_location.raw_source_name | Cells |
| best_oa_location.landing_page_url | https://doi.org/10.3390/cells10112859 |
| primary_location.id | doi:10.3390/cells10112859 |
| primary_location.is_oa | True |
| primary_location.source.id | https://openalex.org/S2736626452 |
| primary_location.source.issn | 2073-4409 |
| primary_location.source.type | journal |
| primary_location.source.is_oa | True |
| primary_location.source.issn_l | 2073-4409 |
| primary_location.source.is_core | True |
| primary_location.source.is_in_doaj | True |
| primary_location.source.display_name | Cells |
| primary_location.source.host_organization | https://openalex.org/P4310310987 |
| primary_location.source.host_organization_name | Multidisciplinary Digital Publishing Institute |
| primary_location.source.host_organization_lineage | https://openalex.org/P4310310987 |
| primary_location.source.host_organization_lineage_names | Multidisciplinary Digital Publishing Institute |
| primary_location.license | cc-by |
| primary_location.pdf_url | https://www.mdpi.com/2073-4409/10/11/2859/pdf?version=1635760753 |
| primary_location.version | publishedVersion |
| primary_location.raw_type | journal-article |
| primary_location.license_id | https://openalex.org/licenses/cc-by |
| primary_location.is_accepted | True |
| primary_location.is_published | True |
| primary_location.raw_source_name | Cells |
| primary_location.landing_page_url | https://doi.org/10.3390/cells10112859 |
| publication_date | 2021-10-23 |
| publication_year | 2021 |
| referenced_works | https://openalex.org/W2792515237, https://openalex.org/W2770583262, https://openalex.org/W2134208433, https://openalex.org/W2980824804, https://openalex.org/W2886751286, https://openalex.org/W2034713721, https://openalex.org/W2737711489, https://openalex.org/W2966311990, https://openalex.org/W3015871332, https://openalex.org/W2757710387, https://openalex.org/W2511744669, https://openalex.org/W2035203405, https://openalex.org/W2137048952, https://openalex.org/W2088128367, https://openalex.org/W2058521801, https://openalex.org/W2609120399, https://openalex.org/W101953629, https://openalex.org/W2871282148, https://openalex.org/W6686863567, https://openalex.org/W2054455698, https://openalex.org/W1992858116, https://openalex.org/W2106316394, https://openalex.org/W2087850846, https://openalex.org/W1969658981, https://openalex.org/W2313159301, https://openalex.org/W1975532391, https://openalex.org/W2915120891, https://openalex.org/W1993666589, https://openalex.org/W2584641334, https://openalex.org/W2022317724, https://openalex.org/W2074347711, https://openalex.org/W2891514442, https://openalex.org/W2127136823, https://openalex.org/W2780153188, https://openalex.org/W2120504118, https://openalex.org/W2167279371, https://openalex.org/W2066521860, https://openalex.org/W1528477098, https://openalex.org/W1969664442, https://openalex.org/W2127722806, https://openalex.org/W2483966273, https://openalex.org/W1780813283, https://openalex.org/W2273560121, https://openalex.org/W2090588873, https://openalex.org/W2173240866, https://openalex.org/W2526835542, https://openalex.org/W6682114833, https://openalex.org/W2088903728, https://openalex.org/W2892285981, https://openalex.org/W3104874466, https://openalex.org/W3100273862, https://openalex.org/W2038573562, https://openalex.org/W2021014465, https://openalex.org/W2186743699, https://openalex.org/W2397071937, https://openalex.org/W2151492748, https://openalex.org/W3098725301 |
| referenced_works_count | 57 |
| abstract_inverted_index.a | 7, 24, 70, 83, 94, 144, 150, 168 |
| abstract_inverted_index.2i | 138 |
| abstract_inverted_index.3D | 113 |
| abstract_inverted_index.We | 126, 176 |
| abstract_inverted_index.as | 31, 45, 69, 82 |
| abstract_inverted_index.be | 106 |
| abstract_inverted_index.by | 47 |
| abstract_inverted_index.ex | 49 |
| abstract_inverted_index.in | 27, 134, 137, 181, 189, 202 |
| abstract_inverted_index.is | 66, 153 |
| abstract_inverted_index.of | 36, 43, 52, 62, 79, 116, 199 |
| abstract_inverted_index.on | 157 |
| abstract_inverted_index.or | 33, 139 |
| abstract_inverted_index.to | 105, 155, 220 |
| abstract_inverted_index.we | 110 |
| abstract_inverted_index.The | 0, 20 |
| abstract_inverted_index.and | 16, 76, 81, 97, 131, 149, 217 |
| abstract_inverted_index.cue | 72 |
| abstract_inverted_index.for | 73, 213 |
| abstract_inverted_index.key | 25 |
| abstract_inverted_index.the | 4, 17, 34, 40, 48, 58, 63, 98, 112, 128, 163, 172, 182, 186, 196, 200, 203, 211 |
| abstract_inverted_index.two | 173, 191, 204 |
| abstract_inverted_index.way | 212 |
| abstract_inverted_index.ESCs | 92, 119, 201 |
| abstract_inverted_index.From | 39 |
| abstract_inverted_index.also | 177 |
| abstract_inverted_index.cell | 5, 37, 147, 183, 221 |
| abstract_inverted_index.have | 93 |
| abstract_inverted_index.pave | 210 |
| abstract_inverted_index.role | 26, 61 |
| abstract_inverted_index.stem | 55 |
| abstract_inverted_index.such | 30 |
| abstract_inverted_index.that | 10, 86, 100, 152, 162 |
| abstract_inverted_index.this | 102 |
| abstract_inverted_index.vivo | 50 |
| abstract_inverted_index.well | 194 |
| abstract_inverted_index.with | 6, 195 |
| abstract_inverted_index.Here, | 109 |
| abstract_inverted_index.STORM | 159 |
| abstract_inverted_index.These | 208 |
| abstract_inverted_index.actin | 21, 64, 114, 165 |
| abstract_inverted_index.cells | 15, 56, 80, 135, 187 |
| abstract_inverted_index.early | 224 |
| abstract_inverted_index.media | 141, 192 |
| abstract_inverted_index.mouse | 118 |
| abstract_inverted_index.plays | 23 |
| abstract_inverted_index.state | 148, 151 |
| abstract_inverted_index.these | 190 |
| abstract_inverted_index.using | 120 |
| abstract_inverted_index.vital | 71 |
| abstract_inverted_index.Naïve | 91 |
| abstract_inverted_index.allows | 11 |
| abstract_inverted_index.change | 170 |
| abstract_inverted_index.closer | 154 |
| abstract_inverted_index.during | 223 |
| abstract_inverted_index.events | 29 |
| abstract_inverted_index.naïve | 53, 117 |
| abstract_inverted_index.showed | 161 |
| abstract_inverted_index.stages | 42 |
| abstract_inverted_index.(ESCs), | 57 |
| abstract_inverted_index.between | 14, 171, 185 |
| abstract_inverted_index.changes | 133 |
| abstract_inverted_index.control | 78 |
| abstract_inverted_index.correct | 74 |
| abstract_inverted_index.culture | 206 |
| abstract_inverted_index.further | 107 |
| abstract_inverted_index.imaging | 160 |
| abstract_inverted_index.lineage | 77 |
| abstract_inverted_index.linking | 214 |
| abstract_inverted_index.optical | 122 |
| abstract_inverted_index.remains | 104 |
| abstract_inverted_index.results | 209 |
| abstract_inverted_index.several | 88 |
| abstract_inverted_index.(STORM). | 125 |
| abstract_inverted_index.becoming | 67 |
| abstract_inverted_index.cellular | 1 |
| abstract_inverted_index.controls | 87 |
| abstract_inverted_index.critical | 59 |
| abstract_inverted_index.cultured | 136, 188 |
| abstract_inverted_index.detected | 178 |
| abstract_inverted_index.dramatic | 169 |
| abstract_inverted_index.earliest | 41 |
| abstract_inverted_index.factors. | 90 |
| abstract_inverted_index.motility | 32 |
| abstract_inverted_index.observed | 197 |
| abstract_inverted_index.physical | 215 |
| abstract_inverted_index.provides | 3 |
| abstract_inverted_index.rigidity | 9 |
| abstract_inverted_index.Serum/LIF | 140 |
| abstract_inverted_index.condition | 103 |
| abstract_inverted_index.culturing | 174 |
| abstract_inverted_index.different | 205 |
| abstract_inverted_index.embarking | 156 |
| abstract_inverted_index.embryonic | 54 |
| abstract_inverted_index.expansion | 51 |
| abstract_inverted_index.periphery | 184 |
| abstract_inverted_index.polarity. | 38 |
| abstract_inverted_index.structure | 22, 65, 85, 166 |
| abstract_inverted_index.undergoes | 167 |
| abstract_inverted_index.underlies | 101 |
| abstract_inverted_index.mechanical | 8, 12, 28, 60, 132 |
| abstract_inverted_index.microscopy | 124 |
| abstract_inverted_index.morphology | 222 |
| abstract_inverted_index.peripheral | 164 |
| abstract_inverted_index.properties | 216 |
| abstract_inverted_index.recognized | 68 |
| abstract_inverted_index.regulatory | 84 |
| abstract_inverted_index.conditions. | 175, 207 |
| abstract_inverted_index.correlating | 193 |
| abstract_inverted_index.differences | 180 |
| abstract_inverted_index.homogeneous | 145 |
| abstract_inverted_index.interaction | 13 |
| abstract_inverted_index.investigate | 111, 127 |
| abstract_inverted_index.morphology, | 96 |
| abstract_inverted_index.reflecting, | 142 |
| abstract_inverted_index.represented | 46 |
| abstract_inverted_index.segregation | 75 |
| abstract_inverted_index.architecture | 219 |
| abstract_inverted_index.cytoskeletal | 218 |
| abstract_inverted_index.cytoskeleton | 2, 115 |
| abstract_inverted_index.development, | 44 |
| abstract_inverted_index.development. | 225 |
| abstract_inverted_index.environment. | 19 |
| abstract_inverted_index.cytoskeletal, | 130 |
| abstract_inverted_index.establishment | 35 |
| abstract_inverted_index.extracellular | 18 |
| abstract_inverted_index.investigated. | 108 |
| abstract_inverted_index.respectively, | 143 |
| abstract_inverted_index.transcription | 89 |
| abstract_inverted_index.characteristic | 95 |
| abstract_inverted_index.morphological, | 129 |
| abstract_inverted_index.reconstruction | 123 |
| abstract_inverted_index.ultrastructure | 99 |
| abstract_inverted_index.preimplantation | 146 |
| abstract_inverted_index.differentiation. | 158 |
| abstract_inverted_index.super-resolution | 121 |
| abstract_inverted_index.micro-rheological | 179 |
| abstract_inverted_index.nano-architecture | 198 |
| cited_by_percentile_year.max | 96 |
| cited_by_percentile_year.min | 90 |
| corresponding_author_ids | https://openalex.org/A5070844214, https://openalex.org/A5029719845 |
| countries_distinct_count | 1 |
| institutions_distinct_count | 6 |
| corresponding_institution_ids | https://openalex.org/I124055696 |
| sustainable_development_goals[0].id | https://metadata.un.org/sdg/15 |
| sustainable_development_goals[0].score | 0.46000000834465027 |
| sustainable_development_goals[0].display_name | Life in Land |
| citation_normalized_percentile.value | 0.55308353 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |